High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus)

Yang, Yang; Liu, Qinghua; Xiao, Yongshuang; Xu, Shihong; Yang, Jingkun; Song, Zongcheng; You, Feng; Li, Jun

doi:10.1016/j.ygcen.2018.12.012

Cited by 23 publications

(12 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although we do not know the function of Gsdf in salmon testis, it is likely that this protein has one or more roles as described in other fish species. One possible role may be associated with testis maturation, since we observed that gsdf expression was higher in immature compared to mature testis, similar to what has been observed in medaka, Japanese flounder, wrasse (Halichoeres trimaculatus) and rice field eel (Monopterus albus) [43,48,54,55]. The fact that we detected a 3.5fold lower number of gsdf reads in testis tissue devoid of germ cells compared to intact testis tissue may suggest a role for gsdf in the communication between germ cells and testicular somatic cells in Atlantic salmon.…”

Section: Gsdfsupporting

confidence: 86%

“…In this study we found that gsdf is highly expressed in females and males, with specific transcript localization in granulosa and Sertoli cells, respectively. Likewise, expression of gsdf in the same cell types has been observed in several fish species including rainbow trout (Oncorhynchus mykiss), medaka (Oryzias latipes), zebrafish, Nile tilapia (Oreochromis niloticus), Olive flounder (Paralichthys olivaceus), Spotted scat (Scatophagus argus) and Japanese flounder (Paralichthys olivaceus) [24,[42][43][44][45][46][47][48]. Transcripts of gsdf have also been detected within oocytes in Olive flounder, and within spermatogonia and spermatids in Chinese tongue sole (Cynoglossus semilaevis) [46,49].…”

Section: Gsdfmentioning

confidence: 83%

See 1 more Smart Citation

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

et al. 2020

View full text Add to dashboard Cite

Background: Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end (dnd). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon. Results: We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf, inha, nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively. Conclusions: This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell-and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha, are located within granulosa and Sertoli cells.

show abstract

Section: Gsdfsupporting

confidence: 86%

Section: Gsdfmentioning

confidence: 83%

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Furthermore, Gsdf expression is high in immature compared to mature testis stages. this finding is similar to what has been observed in other species including Japanese flounder [49], medaka [44], wrasse (Halichoeres trimaculatus) [ Limited information exists on the role of gsdf in females. Targeted disruption of gsdf in medaka causes sterility (abnormal folliculogenesis with restrained oocyte growth and maturation) in 33 out of 38 females.…”

Section: Gsdfsupporting

confidence: 90%

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Kleppe

Edvardsen

Furmanek

et al. 2019

Preprint

View full text Add to dashboard Cite

Background Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild populations. Recently sterile salmon was produced by knocking out the germ cell-specific dead end (dnd). Several approaches may be applied to inhibit Dnd function, including gene knockout, knockdown or immunization. Since it is challenging to develop a successful treatment against a gene product already existing in the body, alternative targets are being explored. Germ cells are surrounded by, and dependent on, gonadal somatic cells. Targeting genes essential for the survival of gonadal somatic cells may be good alternative targets for sterility treatments. Our aim was to identify and characterize novel germ cell and gonadal somatic factors in Atlantic salmon. Results We have for the first time analysed RNA-sequencing data from germ cell-free (GCF)/dnd knockout and wild type (WT) salmon testis and searched for genes preferentially expressed in either germ cells or gonadal somatic cells. To exclude genes with extra-gonadal expression, our dataset was merged with available multi-tissue transcriptome data. We identified 389 gonad specific genes, of which 194 were preferentially expressed within germ cells, and 11 were confined to gonadal somatic cells. Interestingly, 5 of the 11 gonadal somatic transcripts represented genes encoding secreted TGF-β factors; gsdf, inha, nodal and two bmp6-like genes, all representative vaccine targets. Of these, gsdf and inha had the highest transcript levels. Expression of gsdf and inha was further confirmed to be gonad specific, and their spatial expression was restricted to granulosa and Sertoli cells of the ovary and testis, respectively. Finally, we show that inha expression increases with puberty in both ovary and testis tissue, while gsdf expression does not change or decreases during puberty in ovary and testis tissue, respectively. Conclusions This study contributes with transcriptome data on salmon testis tissue with and without germ cells. We provide a list of novel and known germ cell- and gonad somatic specific transcripts, and show that the expression of two highly active gonadal somatic secreted TGF-β factors, gsdf and inha, are located within granulosa and Sertoli cells.

show abstract

“…In addition, Zhang et al (2020) reported that marine medaka Oryzias melastigma exposed to a synthetic estrogen, 17α‐ethynylestradiol, show suppressed gsdf expression in the testis, whereas those exposed to a synthetic androgen, trenbolone, show increased gsdf expression in the ovary. Yang et al (2019) reported that high expression of gsdf might inhibit germ cell proliferation during sex differentiation, and eventually cause sex reversal in the high temperature‐induced masculinization of XX Japanese flounder.…”

Section: Discussionmentioning

confidence: 99%

Exposure to 4‐nonylphenol induces a shift in the gene expression of gsdf and testis‐ova formation and sex reversal in Japanese medaka (Oryzias latipes)

Horie

Kanazawa

Takahashi

et al. 2020

J of Applied Toxicology

View full text Add to dashboard Cite

The branched isomer mixture 4‐nonylphenol (4‐NP) has been used worldwide as a surfactant, and can have endocrine‐disrupting effects on aquatic organisms. For instance, 4‐NP induces the formation of testis‐ova (i.e., testicular and ovarian tissue in the same gonad) or male to female sex reversal of various teleost fishes. Recently, our group revealed that altered gsdf gene expression is associated with disruption of gonadal differentiation in Japanese medaka (Oryzias latipes) embryos exposed to methyltestosterone or bisphenol A, suggesting that gsdf might be useful as a biomarker for predicting the impact of endocrine‐disrupting chemicals (EDCs) on gonadal differentiation. Here, we used 4‐NP to examine further whether gsdf expression at the embryo stage is useful for predicting EDC impact on gonadal sex differentiation. When fertilized medaka eggs were exposed to 32 or 100 μg/L 4‐NP, testis‐ova in genetic males and sex reversal from genetic male to phenotypic female were observed. At stage 38 (just before hatching), 4‐NP exposure at 1‐100 μg/L did not affect gsdf expression in XX embryos compared with the nontreated control; however, in XY embryos, the gsdf expression in the 100 μg/L‐exposed group was significantly lower than that in the controls. The 4‐NP concentration at which gsdf expression was suppressed was equal to that at which testis‐ova and sex reversal were induced. These results indicate that expression of the gsdf gene at the embryonic stage in medaka is a useful biomarker for predicting the impact of EDCs on sexual differentiation.

show abstract

High temperature increases the gsdf expression in masculinization of genetically female Japanese flounder (Paralichthys olivaceus)

Cited by 23 publications

References 39 publications

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Transcriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon

Exposure to 4‐nonylphenol induces a shift in the gene expression of gsdf and testis‐ova formation and sex reversal in Japanese medaka (Oryzias latipes)

Contact Info

Product

Resources

About