High Temperature Extends the Range of Size Discrimination of Nonionic Polymers by a Biological Nanopore

Piguet, Fabien; Ouldali, Hadjer; Discala, Françoise; Breton, Michelyne; Behrends, Jan C.; Pelta, Juan; Oukhaled, Abdelghani

doi:10.1038/srep38675

Cited by 24 publications

(32 citation statements)

References 55 publications

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“…A similar effect is observed upon raising the temperature, where PEG 3400 collapses in size due to decreased solubility. 26 …”

Section: Resultsmentioning

confidence: 99%

“…23–25 An interesting latest development in this single-molecule technique relates to the unexpected temperature dependence of the lifetime of a single polymer molecule trapped by the α -hemolysin (aHL) nanopore. 26 It turned out that the temperature effect can be opposite for PEGs of different molecular weights. For the relatively small PEGs (molecular weight <2000) the time was found to exponentially decrease with temperature, while increasing for the larger PEGs (molecular weight 2000 and higher).…”

Section: Introductionmentioning

confidence: 99%

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Poly(ethylene glycol)s in Semidilute Regime: Radius of Gyration in the Bulk and Partitioning into a Nanopore

Gurnev

Stanley²,

Aksoyoglu

et al. 2017

Macromolecules

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Using two approaches, small-angle neutron scattering (SANS) from bulk solutions and nanopore conductance-fluctuation analysis, we studied structural and dynamic features of poly(ethylene glycol) (PEG) water/salt solutions in the dilute and semidilute regimes. SANS measurements on PEG 3400 at the zero-average contrast yielded the single chain radius of gyration (Rg) over 1–30 wt %. We observed a small but statistically reliable decrease in Rg with increasing PEG concentration: at 30 wt % the chain contracts by a factor of 0.94. Analyzing conductance fluctuations of the α-hemolysin nanopore in the mixtures of PEG 200 with PEG 3400, we demonstrated that polymer partitioning into the nanopore is mostly due to PEG 200. Specifically, for a 1:1 wt/wt mixture the smaller polymer dominates to the extent that only about 1/25 of the nanopore volume is taken by the larger polymer. These findings advance our conceptual and quantitative understanding of nanopore polymer partitioning; they also support the main assumptions of the recent “polymers-pushing-polymers” model.

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“…A similar effect is observed upon raising the temperature, where PEG 3400 collapses in size due to decreased solubility. 26 …”

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Poly(ethylene glycol)s in Semidilute Regime: Radius of Gyration in the Bulk and Partitioning into a Nanopore

Gurnev

Stanley²,

Aksoyoglu

et al. 2017

Macromolecules

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“…We use a device previously described. [37][38][39]. Briefly, we consider two compartments separated by an insulating lipid bilayer and filled with an electrolyte solution, such as KCl buffer.…”

Section: Channel Insertionmentioning

confidence: 99%

From current trace to the understanding of confined media

et al. 2018

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Nanopores constitute devices for the sensing of nano-objects such as ions, polymer chains, proteins or nanoparticles. We describe what information we can extract from the current trace. We consider the entrance of polydisperse chains into the nanopore, which leads to a conductance drop. We describe the detection of these current blockades according to their shape. Finally, we explain how data analysis can be used to enhance our understanding of physical processes in confined media.

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“…An increase in size resolution for larger PEG chains was demonstrated using alpha-hemolysin by increasing the temperature. It is due to the collapse of the polymers inside the pore 118 . For the longer chains, the resolution is progressively lost.…”

Section: Discussion and Perspectivesmentioning

confidence: 99%

Aerolysin, a Powerful Protein Sensor for Fundamental Studies and Development of Upcoming Applications

et al. 2019

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Nanopore electrical approach is a breakthrough in single molecular level detection of particles as small as ions, and complex as biomolecules. This technique can be used for molecule analysis, and characterization as well as for the understanding of confined medium dynamics in chemical or biological reactions. Altogether, the information obtained from these kinds of experiments will allow to address challenges in a variety of biological fields. The sensing, design and manufacture of nanopores is crucial to obtain these objectives. For some time now, aerolysin, a pore forming toxin, and its mutants have shown high potential in real time analytical chemistry, size discrimination of neutral polymers, oligosaccharides, oligonucleotides and peptides at monomeric resolution, sequence identification, chemical modification on DNA, potential biomarkers detection and protein folding analysis. This review focuses, on the results obtained with aerolysin nanopores on the fields of chemistry, biology, physics and biotechnology. We discuss and compare as well the results obtained with other protein channel sensors.For the past two decades, the number of papers and the citations in the field of nanopores has increased exponentially. Nanopore technology has become a sensitive, selective, low cost, label-free, real-time and transportable tool for sensing a wide variety of molecules, including ions, polymers, polyelectrolytes, viruses, ligand-molecule complexes and biomolecules. It allows for the analysis of transport properties, conformations, folding, size, sequence or chemical modifications 1-6 . At first, the protein channels were the main sensors used to perform numerous studies 7-9 . Thanks to material science, chemistry, nanoscience and molecular biology, it is now possible to design and manufacture new classes of nanopores: solid-state sensors 10,11 , DNA origami nanopores 12,13 , carbon 14,15 or cyclodextrin nanotubes [16][17][18] , hybrid nanopores 19-21 and glass 22,23 or quartz nanopores 24 with DNA aptamers 25 . The increased interest in nanopore research has been mainly associated to the ultra-fast DNA sequencing challenge, recently achieved by Oxford Nanopore Technology [26][27][28][29] . The objectives at the horizon of this field comprise of proteomic sequencing 6,30-33 , biomarker detection (of micro-RNAs 34 as well as infinitesimal peptides and proteins quantities) and single-molecule mass spectrometry 32,[35][36][37][38][39][40][41][42] . Up to now, the best sensitivity for biotechnological or heath applications is obtained through biological channels.A member of the pore-forming toxin (PFTs) 43 family, aerolysin is a beta structure toxin that has recently been at the center of extensive fundamental studies and some biotechnology applications 32,38,39,[44][45][46][47][48] . In this review we first introduce the molecular mechanism of channel formation, from soluble inactive monomers to a functional pore into a lipid membrane (figure 1); we delve into the structure of monomeric and heptameric aerolysin along with...

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High Temperature Extends the Range of Size Discrimination of Nonionic Polymers by a Biological Nanopore

Cited by 24 publications

References 55 publications

Poly(ethylene glycol)s in Semidilute Regime: Radius of Gyration in the Bulk and Partitioning into a Nanopore

Poly(ethylene glycol)s in Semidilute Regime: Radius of Gyration in the Bulk and Partitioning into a Nanopore

From current trace to the understanding of confined media

Aerolysin, a Powerful Protein Sensor for Fundamental Studies and Development of Upcoming Applications

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