High-temperature biofilm system based on heterotrophic nitrification and aerobic denitrification treating high-strength ammonia wastewater: Nitrogen removal performances and temperature-regulated metabolic pathways

Lin, Ziyuan; Zhou, Jian; He, Lei; He, Xuejie; Pan, Zhanglei; Wang, Yingmu; He, Qiang

doi:10.1016/j.biortech.2021.126184

Cited by 37 publications

(4 citation statements)

References 43 publications

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“…However, there is not just one nitration and denitration gene during denitrification. The enzyme of a strain might have multiple enzyme genes coexisting [ 40 ]. The strain HS2 possesses both Nap and NarG; similarly, Luz et al [ 41 ] found that Bacillus spp.…”

Section: Discussionmentioning

confidence: 99%

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain Achromobacter spiritinus HS2 and Its Application

Gao,

Zhang,

et al. 2024

Microorganisms

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The low-temperature environment significantly inhibits the growth and metabolism of denitrifying bacteria, leading to an excessive concentration of ammonia nitrogen and total nitrogen in sewage treatment plants during the cold season. In this study, an efficient denitrifying strain of heterotrophic nitrification–aerobic denitrification (HN–AD) bacteria named HS2 was isolated and screened from industrial sewage of a chemical factory in Inner Mongolia at 8 °C. The strain was confirmed to be Achromobacter spiritinus, a colorless rod-shaped bacterium. When cultured with sodium succinate as the carbon source, a carbon-to-nitrogen ratio of 20–30, a shaking rate of 150–180 r/min, and an initial pH of 6–10, the strain HS2 exhibited excellent nitrogen removal at 8 °C. Through the results of whole-genome sequencing, gene amplification, and gas product detection, the strain HS2 was determined to possess key enzyme genes in both nitrification and denitrification pathways, suggesting a HN–AD pathway of NH4+-N → NH2OH → NO2−N → NO → N2O → N2. At 8 °C, the strain HS2 could completely remove ammonia nitrogen from industrial sewage with an initial concentration of 127.23 mg/L. Microbial species diversity analysis of the final sewage confirmed Achromobacter sp. as the dominant genus, which indicated that the low-temperature denitrifying strain HS2 plays an important role in nitrogen removal in actual low-temperature sewage.

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Section: Discussionmentioning

confidence: 99%

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain Achromobacter spiritinus HS2 and Its Application

Gao,

Zhang,

et al. 2024

Microorganisms

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“…But there isn't just one nitration and denitration gene during denitrification. A strain might have multiple enzyme genes coexisting [43]. Luz et al [44] found Bacillus spp.…”

Section: Discussionmentioning

confidence: 99%

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain <em>Achromobacter spiritinus</em> HS2 and Its Application

Gao,

Zhang,

et al. 2024

Preprint

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The low-temperature environment significantly inhibits the growth and metabolism of denitrifying bacteria, leading to an excessive concentration of ammonia nitrogen and total nitrogen in sewage treatment plants during the cold season. In this study, an efficient denitrifying strain of heterotrophic nitrification–aerobic denitrification(HN-AD) bacteria named HS2 was isolated and screened from industrial sewage of a chemical factory in Inner Mongolia at 8°C. The strain was confirmed to be Achromobacter spiritinus, a colorless rod-shaped bacterium. When cultured with sodium succinate as the carbon source, a carbon-to-nitrogen ratio of 20-30, a shaking rate of 150-180 r/min, and the initial pH of 6-10, the Strain HS2 exhibited excellent nitrogen removal at 8°C. Through the results of whole-genome sequencing, gene amplification, and gas product detection, the Strain HS2 was determined to possess key enzyme genes in both nitrification and denitrification pathways, suggesting a HN-AD pathway of NH4+-N→NH2OH→NO2--N→NO→N2O→N2. At 8°C, the Strain HS2 could completely remove ammonia nitrogen from industrial sewage with an initial concentration of 127.23 mg/L. Microbial species diversity analysis of the final sewage confirmed Achromobacter sp. as the dominant genus, this indicated that the low-temperature denitrifying strain HS2 plays an important role in nitrogen removal of actual low-temperature sewage.

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“…It has been reported that C metabolisms interact with denitrification and affect N removal in various systems [13,48]. The up-regulated C metabolism allows the system to generate more energy, thereby promoting the HN-AD-related N removal pathways [49]. On the other hand, the modulated C metabolism optimized electron distribution among denitrifying enzymes by adsorbing the extracellular metabolites [50].…”

Section: Metabolism and N Cycle Genes Of Activated Sludgementioning

confidence: 99%

Enhancement of Microbial and Metabolic Mechanisms in an Aerobic Bioreactor with Immobilized Microflora by Simple and Complex Electron Donors

Deng

Wang²,

Wang

et al. 2023

Water

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Microflora immobilization is promising for nutrient removal applications in sewage; however, the metabolic and microbial mechanism needs to be further explored. Heterotrophic nitrification-aerobic denitrification (HN-AD) bacterium and efficient nitrogen (N) removal bacteria were selected and immobilized on corncob particles using alginate polymer to prepare microbe–organic complex beads. The complex beads were then added into activated sludge under a continuous-flow aerobic bioreactor with sufficient sodium acetate also applied as a simple electron donor. The role of polymer electron donors under carbon-rich conditions was then studied. Results showed that the total nitrogen removal rate improved by 8.3% (reaching 91.2%) and ammonium nitrogen removal rates were approximately 98%. Only 0.59 mg/L of nitrate nitrogen was detected in the treatment group. 16S rRNA gene sequencing results showed that bacterial richness in activated sludge within the treatment group was significantly higher than within the control group (p < 0.05), and KEGG pathways analysis indicated that carbon (C) metabolism gene and N-cycle-related genes were also improved. This suggested that polymer electron donors generated complex C sources that nourished diverse bacterial species related to N cycles so that the N removal rate could be strengthened and further improved by simple electron donors and the microflora.

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High-temperature biofilm system based on heterotrophic nitrification and aerobic denitrification treating high-strength ammonia wastewater: Nitrogen removal performances and temperature-regulated metabolic pathways

Cited by 37 publications

References 43 publications

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain Achromobacter spiritinus HS2 and Its Application

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain Achromobacter spiritinus HS2 and Its Application

Denitrification Characteristics of the Low-Temperature Tolerant Denitrification Strain <em>Achromobacter spiritinus</em> HS2 and Its Application

Enhancement of Microbial and Metabolic Mechanisms in an Aerobic Bioreactor with Immobilized Microflora by Simple and Complex Electron Donors

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