High telomerase activity and high HTRT mRNA expression differentiate pure myxoid and myxoid/round-cell liposarcomas

Schneider‐Stock, Regine; Jaeger, Viola; Ryś, Janusz; Epplen, Jörg T.; Roessner, Albert

doi:10.1002/(sici)1097-0215(20000120)89:1<63::aid-ijc10>3.0.co;2-v

Cited by 25 publications

(19 citation statements)

References 20 publications

(23 reference statements)

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“…2). hTERT expression measured by the previous protocol [8] was significantly correlated with the data obtained by real-time PCR in the LightCycler system, (p = 0.002; Fig. 3).…”

supporting

confidence: 52%

“…In a previous study, we measured hTERT-expression semiquantitatively by end-point RT-PCR using silverstained polyacrylamide gels and error-sensitive densitometric measurements in a group of myxoid liposarcomas [8] in order to find a molecular progression marker in this tumor entity. It is known that myxoid liposarcoma may show a transition from the low malignant pure myxoid to a higher malignant round cell variant [9].…”

mentioning

confidence: 99%

“…hTERT expression levels were quantified using the LightCycler TeloTAGGG hTERT Quantification Kit (Roche Molecular Biochemicals). Briefly, total RNA was extracted using TRIzolReagent (Gibco BRL, Grand Island, NY, USA) according to the manufacturer's instructions [8]. hTERTencoding mRNA was reversely transcribed and amplified in a one-step RT-PCR protocol using the LightCycler Instrument.…”

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confidence: 99%

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Analysis of human telomerase reverse transcriptase mRNA (hTERT) expression in myxoid liposarcomas using LightCycler real-time quantitative reverse transcriptase-polymerase chain reaction

Schneider‐Stock¹,

Emrich²,

Peters³

et al. 2001

Electrophoresis

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We describe a convenient, nonradioactive reverse transcription--polymerase chain reaktion (RT-PCR) method for the rapid and accurate quantitative detection of the human telomerase catalytic subunit human telomerase reverse transcriptase (hTERT) mRNA. The LightCycler TeloTAGGG hTERT Quantification Kit (Roche Molecular Biochemicals) was designed to be used for the highly sensitive and quantitative detection of hTERT mRNA relative to the house-keeping gene porphobilinogen deaminase (PBGD). As a tumor progression model, we investigated 26 myxoid liposarcomas (11 pure myxoid grade I, 15 myxoid/round cell grade II/III) for the hTERT expression level and compared the results of the new method with former measurements performed in silver-stained polyacrylamide gels. Both methods revealed similar results, with real-time RT-PCR being the more accurate quantification technique, which also saves time and material. Elevated hTERT expression (cut-off ratio x 100 at 1.3) was an indicator of round cell components and hence for tumor progression in myxoid liposarcoma. The new method is capable of differentiating between pure myxoid and myxoid/round cell liposarcomas for hTERT-expression more accurately.

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“…2). hTERT expression measured by the previous protocol [8] was significantly correlated with the data obtained by real-time PCR in the LightCycler system, (p = 0.002; Fig. 3).…”

supporting

confidence: 52%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Analysis of human telomerase reverse transcriptase mRNA (hTERT) expression in myxoid liposarcomas using LightCycler real-time quantitative reverse transcriptase-polymerase chain reaction

Schneider‐Stock¹,

Emrich²,

Peters³

et al. 2001

Electrophoresis

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“…5 A rapid literature survey shows, however, that TA in STS may vary greatly, ranging from 17% 7 to 100% 9 of the cases examined. Some authors also found a positive correlation between TA and tumor aggressiveness in sarcomas 4 and, more specifically, in liposarcomas 5,6,9 whereas others did not. 3 Telomere length has rarely been examined in STS.…”

mentioning

confidence: 96%

“…Telomerase activity (TA) has been shown to be expressed in a majority of human carcinomas 1,2 but only few data exist regarding TA in sarcomas. [3][4][5][6][7][8][9] In a previous study, 3 we showed that TA is detectable in only about 50% of intermediate-/high-grade soft tissue sarcomas (STS) and undetectable in benign lesions and low-grade STS. These results are in keeping with those of Schneider Stock et al detecting TA in 38% (23/60) of their STS 4 and 69% of liposarcomas.…”

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confidence: 99%

Telomerase activity and hTERT mRNA expression can be heterogeneous and does not correlate with telomere length in soft tissue sarcomas

Yan

Benhattar

Coindre

et al. 2002

Intl Journal of Cancer

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In a previous study, we showed that telomerase activity (TA) and human telomerase reverse transcriptase (hTERT) mRNA expression were undetectable in benign mesenchymal lesions and low-grade soft tissue sarcomas (STSs), but detectable in about 50% of intermediate-/high-grade STSs. We wondered if this lack of TA or hTERT mRNA expression could be related to the tumor sample examined and if there was a relationship between the former 2 parameters and telomere length. Two separate tumor samples from 37 STSs were examined for telomerase activity, using the telomerase repeat amplification protocol (TRAP) assay and for hTERT mRNA expression, using RT-PCR. Considerable attention has been drawn recently to the regulation of telomere length and telomerase activity in the process of cellular senescence and immortalization. 1,2 Human telomeres are specialized terminal chromosomal structures composed of TTAGGG repeats. Shortening of telomeres has been suggested to represent the mitotic clock of cell cycle; telomere shortening is observed in most dividing somatic cells, leading to cell senescence when reaching a critical level. 1,2 Telomerase, a ribonucleoprotein enzyme that is capable of synthesizing telomeric repeats, plays a pivotal role in the process of telomere length maintenance and cell immortalization. Telomerase activity (TA) has been shown to be expressed in a majority of human carcinomas 1,2 but only few data exist regarding TA in sarcomas. [3][4][5][6][7][8][9] In a previous study, 3 we showed that TA is detectable in only about 50% of intermediate-/high-grade soft tissue sarcomas (STS) and undetectable in benign lesions and low-grade STS. These results are in keeping with those of Schneider Stock et al. detecting TA in 38% (23/60) of their STS 4 and 69% of liposarcomas. 5 A rapid literature survey shows, however, that TA in STS may vary greatly, ranging from 17% 7 to 100% 9 of the cases examined. Some authors also found a positive correlation between TA and tumor aggressiveness in sarcomas 4 and, more specifically, in liposarcomas 5,6,9 whereas others did not. 3 Telomere length has rarely been examined in STS. 5,10 Examining a series of 30 sarcomas, Schneider-Stock et al. 10 showed that telomere TRF length was heterogeneous in this tumor category. Unfortunately, they didn't examine their tumors for TA and, thus, it was not known if telomere length correlated with TA or not. In a further study focusing on liposarcomas only, the same group found a positive correlation between the presence of TA, tumor aggressiveness, high tumor grade and high proliferative activity but not between TA and TRF length. This lack of correlation between TA and TRF length may have several causes including the activation of telomerase-independent mechanism(s) of telomere lengthening referred to as alternative lengthening of telomeres (ALT) 11-13 that seems to occur by means of telomeretelomere recombination. 13,14 In our first study on STS, we observed some degree of heterogeneity in TA expression when examining several, otherwise morphol...

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Presence of telomerase activity in different musculoskeletal tumor histotypes and correlation with aggressiveness

et al. 2001

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Telomerase is a ribonucleoprotein enzyme that maintains the protective structures at the ends of eukaryotic chromosomes, called telomeres. Telomerase activity was observed and correlated with aggressiveness in different neoplasms such as breast, prostate, blood and brain cancers, among others. To investigate whether telomerase activity is an index of aggressiveness in bone and soft tissue lesions of the extremities, 66 biopsy samples from our tissue bank were studied. These samples included 43 high‐grade sarcomas, 9 aggressive benign tumors and 14 totally benign lesions. The samples were collected from patients homogeneously treated at the Rizzoli Orthopaedic Institute with a follow‐up ranging from 4 to 11 years (median, 7 years). A non‐radioactive polymerase chain reaction‐based enzyme‐linked immunosorbent assay was used for the study. All tumors investigated were positive for telomerase activity. Among benign lesions, only 2 aneurysmal bone cysts showed higher telomerase activity than the cut‐off point, whereas all the other benign lesions had lower activity. Our results indicate that high levels of telomerase activity in bone and soft tissue lesions correlate with more aggressive clinical behavior in patients treated with surgery alone. An interesting inverse correlation between telomerase activity and occurrence of pulmonary metastasis was detected in osteosarcoma patients treated with chemotherapy. A parallel increase of telomerase activity and malignancy was observed in the adipose and cartilagineous tissue lesions. Our data suggest that telomerase activity could be considered a marker of tumor aggressiveness for bone and soft tissue lesions. The results obtained in osteosarcoma samples suggest that low levels of telomerase activity may be predictive of the prognosis and should influence the therapeutic protocol. © 2001 Wiley‐Liss, Inc.

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High telomerase activity and high HTRT mRNA expression differentiate pure myxoid and myxoid/round-cell liposarcomas

Cited by 25 publications

References 20 publications

Analysis of human telomerase reverse transcriptase mRNA (hTERT) expression in myxoid liposarcomas using LightCycler real-time quantitative reverse transcriptase-polymerase chain reaction

Analysis of human telomerase reverse transcriptase mRNA (hTERT) expression in myxoid liposarcomas using LightCycler real-time quantitative reverse transcriptase-polymerase chain reaction

Telomerase activity and hTERT mRNA expression can be heterogeneous and does not correlate with telomere length in soft tissue sarcomas

Presence of telomerase activity in different musculoskeletal tumor histotypes and correlation with aggressiveness

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