High susceptibility to liver injury in IL-27 p28 conditional knockout mice involves intrinsic interferon-γ dysregulation of CD4⁺T cells

Critical Role of Dendritic Cell–Derived IL-27 in Antitumor Immunity through Regulating the Recruitment and Activation of NK and NKT Cells

“…*p , 0.05, **p , 0.01, ***p , 0.001. munity in both a chemical carcinogenesis model and a transplanted melanoma model. Given the fact that IL-27 is the only well-defined functional cytokine that is composed of IL-27p28 in vivo, we used IL-27p28 flox/flox conditional KO mouse generated in our laboratory (28). Studies in Itgax-p28 f/f mice proved that DCs were the cellular source of IL-27 in antitumor immunity.…”

“…IL-27p28 flox/flox mice in the 129/Sv genetic background were generated in our laboratory with the help of X. Gao (Model Animal Research Center, Nanjing University) and backcrossed to the C57BL/6 strain for 10 generations (28). IL-27p28…”

“…To study the biological function of IL-27, we used IL-27p28 flox/flox mice (hereafter referred to as WT mice) generated in our laboratory (28), which have been crossed with EIIa-Cre mice (30) and CD11c/ Itgax-Cre mice (31) to delete IL-27p28 alleles in nearly all tissues (hereafter referred to as EIIa-p28 f/f mice) and especially in DCs (hereafter referred to as Itgax-p28 f/f mice), respectively. To directly evaluate the importance of host-derived IL-27 in tumor development, we tested WT, EIIa-p28…”

“…In this study, we used IL-27p28 flox/flox conditional knockout (KO) mice (28) to examine the physiological function of endogenous IL-27 in tumor immunity using methyl-cholanthrene (MCA)-induced fibrosarcoma and transplanted B16 melanoma models. We identified a novel role of dendritic cell (DC)-derived IL-27 in antitumor immunity through shaping tumor microenvironment to regulate the recruitment and activation of NK and NKT cells.…”

MiR-29a and miR-652 Attenuate Liver Fibrosis by Inhibiting the Differentiation of CD4+ T Cells

“…Tel: +86-025-85816332 E-mail: wenweiabc@yeah.net CD4+ T cells were a set of cells that involved in the host immune system through liberating the cytokines such as T helper17 (Th17) (Schenten et al, 2014). Recently study has proved that the percent of CD4+ T cells were affecting the liver inflammation and fibrosis (Zhang et al, 2013). ) is a pro-inflammatory cell that plays vital role on host defenses and several autoimmune disorders through releasing of cytokines such as , and both IL-17A and IL-22 functioned to control of extracellular bacterial infection (Shigematsu et al, 2013).…”