High survival rate of vitrified human oocytes results in clinical pregnancy

Katayama, K. Paul; Stehlik, Joni; Kuwayama, Masashige; Kato, Osamu; Stehlik, Ed

doi:10.1016/s0015-0282(03)00551-x

Cited by 244 publications

(119 citation statements)

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“…Although the results for fertilization and embryonic development of human oocytes after vitrification were not available for the present, a 100% survival rate was observed for both immature and mature human oocytes following vitrification with a mixture of 15% EG and 15% PROH using a Cryotop (Data not shown). This further confirms that a high survival rate can be achieved from vitrified human oocytes using Cryotop [13], indicating that vitrification may be an efficient method for cryopreservation of human oocytes.…”

Section: Discussionsupporting

confidence: 70%

“…The results of the present study support this hypothesis. Although the mixture of 15% EG and 15% DMSO is effective to vitrify human oocytes [13,32] and embryos [30,31], our result indicates that a mixture with a final concentration of 15% EG and 15% PROH is more efficient for oocyte vitrification than the mixture of 15% EG and 15% DMSO (Table 1). To the best of our knowledge, this is the first report that bovine oocytes can be vitrified successfully with the mixture of EG and PROH.…”

Section: Discussionmentioning

confidence: 76%

“…It would be a useful treatment option for women who are at risk of losing their ovarian function because of pelvic diseases, surgery or radio/ chemotherapy and could also avoid some of the moral and ethical problems posed by embryo freezing. Although several pregnancies and live births following freezing and thawing of human oocytes have been reported [1][2][3][4][5][6][7][8][9][10][11][12][13], human oocyte freezing as a technology is still considered to be at an early developmental stage. Improving pregnancy rates associated with the use of cryopreserved human oocytes would be a major advance in human ART.…”

mentioning

confidence: 99%

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High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Chian

Kuwayama

Tan

et al. 2004

Journal of Reproduction and Development

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165

100

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Abstract. Improving pregnancy rates associated with the use of cryopreserved human oocytes would be an important advance in human assisted reproductive technology (ART). Vitrification allows glasslike solidification of a solution without ice crystal formation in the living cells. We have attempted to improve the survival rates of oocytes by a vitrification technique using bovine models. In vitro matured oocytes with or without cumulus cells were vitrified with either 15.0% (v/v) ethylene glycol (EG) + 15% (v/v) dimethylsulfoxide (DMSO) + 0.5 M sucrose or 15% (v/v) EG + 15% (v/v) 1,2-propanediol (PROH) + 0.5 M sucrose, using 'Cryotop' or 'thin plastic sticker', respectively. The oocyte survival rates after vitrifying-warming, and the capacity for fertilization and embryonic development were examined in vitro. The rate of embryonic development to blastocyst was significantly higher (P<0.05) in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) PROH + 0.5 M sucrose than in the oocytes vitrified with 15% (v/v) EG + 15% (v/v) DMSO + 0.5 M sucrose (7.4% ± 4.1 vs. 1.7% ± 3.0, respectively). Oocytes vitrified without cumulus cells had a higher survival rate after thawing and a superior embryonic developmental capacity compared with oocytes vitrified with cumulus cells. Prolonged pre-incubation time after thawing adversely affected the rates of embryonic cleavage and development. These results indicate that in vitro matured bovine oocytes can be vitrified successfully with the mixture of the cryoprotectants, EG + PROH, the absence of cumulus cells for vitrification does not affect oocyte survival rate after warming, and vitrified and warmed oocytes do not require pre-incubation before in vitro fertilization.

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 76%

mentioning

confidence: 99%

See 1 more Smart Citation

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Chian

Kuwayama

Tan

et al. 2004

Journal of Reproduction and Development

Self Cite

165

100

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show abstract

“…Cryopreservation may lead to the premature release of cortical granules, hardening of the zona, and depolymerization of meiotic spindles (1). In recent years, modifications of the slow-freezing method or vitrification have significantly improved the survival rate (2)(3)(4)(5)(6). Intracytoplasmic sperm injection (ICSI) is widely used as an effective technique to achieve fertilization for cryopreserved oocytes.…”

Section: Introductionmentioning

confidence: 99%

Short Communication: Cryopreserved Sibling Oocytes and Intracytoplasmic Sperm Injection Rescue Unexpectedly Poor Fertilization in Conventional In Vitro Fertilization

Chen

Lien

Chang

et al. 2004

J Assist Reprod Genet

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Purpose : To report a successful pregnancy from cryopreserved sibling oocytes and intracytoplasmic sperm injection (ICSI) for an infertile couple with an unexpectedly low fertilization rate in the fresh in vitro fertilization (IVF) cycle. Methods : The woman had bilateral tubal obstruction and polycystic ovarian syndrome. The man had normal semen parameters. The couple underwent a cycle of controlled ovarian hyperstimulation in that 20 oocytes were retrieved. Twelve oocytes were conventionally inseminated and eight were cryopreserved using a slow freezing method. However, only one oocyte was fertilized, and no pregnancy was achieved. In the next cycle, the frozen oocytes were thawed and ICSI was performed. Results : After thawing, seven oocytes (88%) survived and one was damaged. Six were at the metaphase II stage and were injected. Five (83%) achieved normal fertilization, and all of them cleaved (100%). After replacement of the embryos, a singleton pregnancy developed. A healthy female baby was delivered at term. Karyotyping revealed 46, XX. Conclusions : In addition to well-known indications, cryopreservation of excess sibling oocytes for patients receiving IVF has a possible advantage of preventing unexpectedly low fertilization rate or fertilization failures.

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“…Although alternative low toxicity vitrification solutions were first reported in 1990 [39], nearly a decade passed before being proposed for improved assisted reproductive technologies application on human oocytes, cleaved-embryos and blastocysts [40,41]. This interest in clinical vitrification was linked to the formation of unique cryo devices like the open pulled straw [42,43], cryoloops [44][45][46] and cryotops [47,48], whose thin surrounding film of vitrification solution and direct contact with LN 2 achieved ultrarapid cooling rates. Furthermore, our understanding of vitrification solutions and their potential toxicity were enhanced by Drs.…”

Section: Vitrificationmentioning

confidence: 99%

The Historic Development and Incorporation of Four Assisted Reproductive Technologies Shaping Today′s IVF Industry

Schiewe¹

2016

JFIV Reprod Med Genet

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The concept of assisted reproductive technologies was most notably derived from the late 19th Century experiments of Sir Walter Heape who successfully transplanted rabbit embryos. Interestingly, it was not until the late 1940's and 1950's that renewed interests in rabbit embryo transfer and cryobiology occurred. The history behind developing effective procedures can be fascinating, though few could be more accidental than Dr. Chris Polge's discovery of glycerol (1948), from a mislabeled bottle of sugar solutions, being an effective cryoprotective agent for sperm freezing. The purpose of this review paper is to discuss four key scientific breakthrough technologies occurring between 1985 and 1995, which ultimately shaped the future of today's human in vitro fertilization (IVF) industry. More importantly, this paper highlights the foundation of underlying related discoveries and some unique stories involving their development and publication. In the end, this paper emphasizes the value of understanding scientific discovery timetables and the eventual re-discovery in the hands and minds of creative, determined and dedicated scientists, as history tends to repeat itself before its useful application is realized.

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High survival rate of vitrified human oocytes results in clinical pregnancy

Cited by 244 publications

References 7 publications

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

High Survival Rate of Bovine Oocytes Matured In Vitro Following Vitrification

Short Communication: Cryopreserved Sibling Oocytes and Intracytoplasmic Sperm Injection Rescue Unexpectedly Poor Fertilization in Conventional In Vitro Fertilization

The Historic Development and Incorporation of Four Assisted Reproductive Technologies Shaping Today′s IVF Industry

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