High survival rate of unfertilized mouse oocytes after vitrification

Abstract

Unfertilized mouse oocytes were cooled rapidly by directly plunging them into liquid nitrogen, immediately after exposure to a highly concentrated solution (modified VS1: 2.53 M-dimethyl sulphoxide, 2.36 M-acetamide, 1.19 M-propylene glycol, 5.4% (w/v) polyethylene glycol (Mr 8000) in PB1), and later warmed in a 37 degree C waterbath. After warming, 305 out of 348 oocytes (87.6%) were morphologically normal. After fertilization in vitro of cryopreserved oocytes, the proportions of pronuclear oocytes and 2-cell embryos 5 and 24 h after insemination were 81.6% (124/152) and 78.4% (120/153), respectively. All 2-cell embryos obtained from cryopreserved oocytes were transferred to the oviducts of pseudopregnant recipients and 45.8% (55/120) developed to normal young.

Overcoming a Permeability Barrier by Microinjecting Cryoprotectants into Zebrafish Embryos (Brachydanio rerio)

“…They found that the addition of an amide to McjSO mitigated its toxicity (3,5,6,9). As a result, investigators have used amides to reduce the toxicity of McjSO in a variety of systems, such as rat kidney renal cortex (3), rabbit renal cortex (5, 6), porcine blastocysts (36), rat blastocysts (19), bovine oocytes (10), mouse oocytes (22,24), and mouse embryos (23). Thus it seemed promising to use amides in zebrafish embryos.…”

Cryopreservation of Unfertilized Mouse Oocytes from Inbred Strains by Ultrarapid Freezing

“…This auther has previously demonstrated that unfertilized mouse oocytes from F1 hybrid strain can be cryopreserved successfully using the method of ultrarapid freezing [4].…”

High incidence of multiple aster formation in vitrified-warmed bovine oocytes after in vitro fertilization

“…Treatment with CPA induced a transient rise of intracellular free calcium level, premature exocytosis of cortical granules, and hardening of zonae pellucidae [7,8]. Application of vitrification improved the efficacy of oocyte cryopreservation, especially in mice [9,10] and humans [11,12]. However, vitrification of oocytes from large domestic species enriched with cytoplasmic lipid droplets still requires substantial improvement [13][14][15][16][17].…”