High‐strength functionalized pectin/fibroin hydrogel with tunable properties: A structure–property relationship study

Abstract

Hydrogels possessing high mechanical strength and well-controlled properties are of utmost interest in bone tissue engineering. In the current study, a strong self-crosslinked hydrogel with variable properties has been developed from a combinatory composition of functionalized pectin and silk fibroin. Pectin chains underwent partial oxidation to be decorated with aldehyde functionalities. Later on, functionalized polysaccharide and protein were blended with different ratios, and corresponding variations in crystalline structure as well as in morphological, mechanical, and rheological properties have been monitored. Some theories have been used to better perceive the relation between blending ratio and hydrogel properties. Avrami equation based on time sweep rheological analysis elucidated that in sample with equivalent ratio of oxidized pectin and fibroin, the crystallization was highly delayed due to dominance of Schiff's base reaction. The Herschel-Bulkley model based on flow curve rheology test corroborated that aforesaid composition revealed the most noticeable shear thinning behavior and accordingly the strongest crosslinked network. Finally, rubber elastic theory based on dynamic mechanical analysis implied that said formulation possessed the highest crosslinking density due to higher availability of aldehyde to amine functionalities. Moreover, cell culture studies proved that P50 F50 sample prevailed over other compositions with respect to cell viability and function.

A GelMA-PEGDA-nHA Composite Hydrogel for Bone Tissue Engineering

“…Many synthetic polymers and natural derivative materials can be used to synthesize hydrogels [ 21 , 23 ]. By modifying the method and degree of cross-linking, the degradation rate, porosity, and mechanical properties can be easily controlled [ 24 ]. The hydrogel prepared by combining natural polymer, synthetic polymer, and HA has achieved good results in bone repair experiments [ 25 , 26 ].…”

Cross-Linked Gelatine by Modified Dextran as a Potential Bioink Prepared by a Simple and Non-Toxic Process

“…Thus, any changes occurring in the material are reversible, even though such behavior is atypical in chemical hydrogels. Nevertheless, Khorsidi et al [53] have found a growing number of aminealdehyde cross-links to correlate with the increased shear-thinning character of hydrogels. Another research found that similar material compositions to the ones examined in the current study can be printed by microextrusion; therefore, a certain level of shear-thinning behavior can be assumed [54].…”

Влияние Строения Пектиновых Веществ Льняных Кормовых Добавок На Абсорбционное Связывание Азагетероциклических Микотоксинов

“…Сопоставлять следует константы k D1 и k D2 между собой (характер взаимодействия с адсорбционными центрами одинаков), а также величину соотно-шения констант для разных субстратов. Для группы тканевых пектинов величина относительного показателя примерно одинакова (1,26). Для P MIGR соотношение констант возросло до 2,04, что свидетельствует одновременно об отсутствии препятствий для поддержания градиента концентрации сорбата в поверхностном слое и о повышенном сопротивлении для протекания внутридиффузионных процессов.…”

“…Наряду с традиционными сферами применения биополимера в пищевой и фармацевтической промышленности растет интерес к использованию его способности к различным видам взаимодействий для получения новых функционализированных материалов. В частности, разработка специальных методов модификации обеспечивает получение самосшитых гидрогелей пектина с фиброином шелка, обладающих высокой механической прочностью и переменными свойствами, которые представляют интерес в инженерии костной ткани [1]. Управление структурной организацией гидрогелей пектина с хитозаном и нанокристаллической целлюлозой позволяет получать биофункциональные инъекционные материалы для тканевой инженерии [2].…”