High-strength, antibacterial, antioxidant, hemostatic, and biocompatible chitin/PEGDE-tannic acid hydrogels for wound healing

Cao, Hankun; Du, Xiuli; Zhou, Xin; Yue, Pengpeng; Zou, Yongkang; Zhong, Zibiao; Ma, Yuexin; Wang, Lizhe; Wu, Shyi-Kuen; Ye, Qifa

doi:10.1016/j.carbpol.2023.120609

Cited by 43 publications

(24 citation statements)

References 90 publications

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“…NIH-3T3 cells were seeded in a 96-well plate at a density of 1500 cells/well and incubated in DMEM plus 10% FBS (v/v) at 37 °C and 5% CO 2 for 24 h, followed by the replacement of the culture medium with 100 μL of the freshly prepared leaching solution. Cells were cultured in the leaching solutions for 1, 2, 3, and 7 days and CCK-8 assay was then conducted to determine cell growth by measuring the absorbance at 450 nm using a Plate Reader (SynergyH1/H1M) . The LIVE/DEAD cell viability assay was performed with cells cultured in the leaching solution of the PAAm-ELN 1.0 -TA 0.2 -CS 0.06 hydrogel by staining the live and dead cells at different time intervals (1, 2, 3, and 7 days), respectively. , The stained cells were subsequently imaged using an inverted fluorescence microscope with excitation/emission at 495/515 and 495/635 nm for live and dead cells, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The hemocompatibility of the hydrogels was evaluated against healthy human red blood cells collected from the Institute of Translational Medicine, First Hospital of Jilin University. ,, Five milliliters of fresh red blood cells were collected by centrifugation at 1000 rpm for 5 min and resuspended in 98 mL of PBS solution (10 mM, pH 7.4). The cylinder-shaped PAAm-ELN 1.0 -TA 0.2 -CS 0.06 , PAAm-ELN 1.0 -TA 0.2 , PAAm-ELN 1.0 , or PAAm hydrogel, with a diameter of 8 mm and height of 3 mm, was sterilized and added into the freshly prepared blood sample followed by incubation at 37 °C for 3 h. The red blood cells incubated in PBS solutions and deionized water in the absence of hydrogels were taken as the negative and positive control, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…The antimicrobial activity of the hydrogels was evaluated using the disk diffusion method. 43,46 Two bacterial strains, S. aureus and E. coli, were cultured in LB medium at 37 °C and 200 rpm for 12 h, respectively. Hundred microliters of bacterial suspension (1 × 10 6 CFU/mL) of each bacterial strain were used to lawn the LB agar plates.…”

Section: = ×mentioning

confidence: 99%

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One-Pot Preparation of Skin-Inspired Multifunctional Hybrid Hydrogel with Robust Wound Healing Capacity

Yang,

Wang,

Zhang

et al. 2023

ACS Biomater. Sci. Eng.

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Bioinspired hydrogels have demonstrated multiple superiorities over traditional wound dressings for wound healing applications. However, the fabrication of bioinspired hydrogel-based wound dressings with desired functionalities always requires multiple successive steps, time-consuming processes, and/or sophisticated protocols, plaguing their clinical applications. Here, a facile one-pot strategy is developed to prepare a skin-inspired multifunctional hydrogel within 30 min by incorporating elastin (an essential functional component of the dermal extracellular matrix), tannic acid, and chitosan into the covalently cross-linked poly(acrylamide) network through noncovalent interactions. The resulting hydrogel exhibits a Young’s modulus (ca. 36 kPa) comparable to that of human skin, a high elongation-at-break (ca. 1550%), a satisfactory tensile strength (ca. 61 kPa), and excellent elastic self-restorability, enabling the hydrogel to synchronously and conformally deform with human skin when used as wound dressings. Importantly, the hydrogel displays a self-adhesive property to skin tissues with an appropriate bonding strength (ca. 55 kPa measured on intact porcine skin), endowing the hydrogel with the ability to rapidly self-adhere to intact human skin, sealing the wound surface and also easily being removed without residue left or trauma caused to the skin. The hydrogel also possesses remarkable antibacterial activity, antioxidant capability, and hemocompatibility. All of these collective beneficial properties enable the hydrogel to significantly accelerate the wound healing process, outperforming the commercial wound dressings.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

One-Pot Preparation of Skin-Inspired Multifunctional Hybrid Hydrogel with Robust Wound Healing Capacity

Yang,

Wang,

Zhang

et al. 2023

ACS Biomater. Sci. Eng.

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“…Therefore, TA can be combined with biopolymers such as collagen, chitosan, and silk protein through noncovalent interactions 46–49 . Phenolic hydroxyl groups can also form hydrogen bonds with water 50 . Thus TA was introduced into the hydrogel network as a second physical cross‐linker to improve mechanical properties and biological functions 42,51–53 .…”

Section: Introductionmentioning

confidence: 99%

“…[46][47][48][49] Phenolic hydroxyl groups can also form hydrogen bonds with water. 50 Thus TA was introduced into the hydrogel network as a second physical cross-linker to improve mechanical properties and biological functions. 42,[51][52][53] Zhao et al 54 prepared TA-modified gelatin hydrogels, and the resulting hydrogels exhibited versatility in self-healing and adhesive properties.…”

mentioning

confidence: 99%

Improving the mechanical properties of 3D printed GelMA composite hydrogels by tannic acid

Zhang

Xiong

et al. 2023

MedComm – Biomaterials and Applications

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The lack of advanced biomaterials is a major challenge in bio‐printing. Gelatin‐methacryloyl (GelMA) hydrogel, as one of the most commonly used biomaterials in 3D printing, has limited the applications of medicine because of its low mechanical properties. In this study, to enhance the mechanical strength of GelMA hydrogels, we prepared a composite hydrogel based on F127 diacrylate (F127DA) and GelMA, followed by lyophilization and tannic acid (TA) treatment. In this composite hydrogel, the F127DA could self‐assemble into nanomicelles as crosslinking centers for monomer polymerization, which provides additional energy dissipation in hydrogels due to the synergistic deformation of micelles and internal rearrangement of physical binding. After lyophilization of the composite hydrogel, the porous hydrogel was formed. The subsequent treatment of TA could diffuse into the inner of the hydrogel and react with the hydrogel via hydrogen bonds, resulting in the significant enhancement of mechanical properties. The maximum tensile deformation of the obtained hydrogel was about 11 times higher than that of GelMA. This work demonstrates a method to enhance the mechanical properties of 3D‐printed GelMA hydrogel with promising application in bioprinting.

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Antioxidant Hydrogels: Antioxidant Mechanisms, Design Strategies, and Applications in the Treatment of Oxidative Stress‐Related Diseases

Hu,

Ouyang,

Zhao

et al. 2024

Adv Healthcare Materials

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Oxidative stress is a biochemical process that disrupts the redox balance due to an excess of oxidized substances within the cell. Oxidative stress is closely associated with a multitude of diseases and health issues, including cancer, diabetes, cardiovascular diseases, neurodegenerative disorders, inflammatory conditions, and aging. Therefore, the developing of antioxidant treatment strategies has emerged as a pivotal area of medical research. Hydrogels have garnered considerable attention due to their exceptional biocompatibility, adjustable physicochemical properties, and capabilities for drug delivery. Numerous antioxidant hydrogels have been developed and proven effective in alleviating oxidative stress. In the pursuit of more effective treatments for oxidative stress‐related diseases, there is an urgent need for advanced strategies for the fabrication of multifunctional antioxidant hydrogels. Consequently, our focus will be on hydrogels that possess exceptional reactive oxygen species (ROS) and reactive nitrogen species (RNS) scavenging capabilities, and their role in oxidative stress therapy will be evaluated. Herein, the antioxidant mechanisms and the design strategies of antioxidant hydrogels and their applications in oxidative stress‐related diseases are discussed systematically in order to provide critical insights for further advancements in the field.This article is protected by copyright. All rights reserved

show abstract

High-strength, antibacterial, antioxidant, hemostatic, and biocompatible chitin/PEGDE-tannic acid hydrogels for wound healing

Cited by 43 publications

References 90 publications

One-Pot Preparation of Skin-Inspired Multifunctional Hybrid Hydrogel with Robust Wound Healing Capacity

One-Pot Preparation of Skin-Inspired Multifunctional Hybrid Hydrogel with Robust Wound Healing Capacity

Improving the mechanical properties of 3D printed GelMA composite hydrogels by tannic acid

Antioxidant Hydrogels: Antioxidant Mechanisms, Design Strategies, and Applications in the Treatment of Oxidative Stress‐Related Diseases

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