High sPLA2-IIA level is associated with eicosanoid metabolism in patients with bacterial sepsis syndrome

Abstract

The aim of this study was to determine the association between secretory phospholipase A2 group IIA (sPLA2-IIA) and eicosanoid pathway metabolites in patients with bacterial sepsis syndrome (BSS). Levels of sPLA2-IIA, eicosanoids prostaglandin (PG)E2, PGD synthase were quantified in the sera from patients confirmed to have bacterial sepsis (BS; N = 45), bacterial severe sepsis/septic shock (BSS/SS; N = 35) and healthy subjects (N = 45). Cyclooxygenase (COX)-1 and COX-2 activities were analyzed from cell lysate. Serum levels of sPLA2-IIA, PGE2, and PGDS increased significantly in patients with BS and BSS/ SS compared to healthy subjects (p<0.05). COX-2 activity was significantly increased in patients with BS compared to healthy subjects (p<0.05), but not COX-1 activity. Binary logistic regression analysis showed that sPLA2-IIA and PGE2 were independent factors predicting BSS severity. In conclusion, high level of sPLA2-IIA is associated with eicosanoid metabolism in patients with BSS.

The lipid biology of sepsis

“…Serum levels of the prostaglandins PGE 2 and PGD 2 are almost doubled in human sepsis subjects. This elevated AA-derived eicosanoid production in human sepsis is accompanied by increased secretory PLA 2 group IIA and COX-2 activity (25).…”

Secretory Phospholipase A2 Enzymes in Acute Lung Injury

“…The circulating levels of sPLA 2 -IIA are very low in healthy conditions but increase significantly during inflammation or infection [13,14]. Several studies have highlighted the role of sPLA 2 -IIA as a biomarker in sepsis and various infections [14][15][16]. sPLA 2 -IIA is also well-recognized for its antimicrobial role, especially toward gram-positive bacteria since it can directly hydrolyze the membranes of these organisms, which are enriched in PG and PE, the preferred substrates for this specific isoform [17].…”

Inflammatory Effects of Bothrops Phospholipases A2: Mechanisms Involved in Biosynthesis of Lipid Mediators and Lipid Accumulation

“…Investigations into the biological role of mammalian group IIA PLA 2 , also known as inflammatory PLA 2 , in the development of several pathologies of inflammatory and immunological origin have been described. Several studies have revealed that group IIA sPLA 2 s are present in high levels in rheumatoid arthritis [47][48][49][50], acute pancreatitis [51][52][53], septic shock [54,55], Crohn's disease and ulcerative colitis [56][57][58], respiratory distress syndrome [59][60][61], bronchial asthma and allergic rhinitis [59,62], atherosclerosis [63,64], autoimmune diseases [65], and cancer [66][67][68][69]. These observations imply that both local and systemic inflammation are associated with the release of sPLA 2 s in vivo, thus raising the unclear question of the role of these PLA 2 s in inflammatory reactions.…”