High-speed volumetric imaging of neuronal activity in freely moving rodents

Skocek, Oliver; Nöbauer, Tobias; Weilguny, Lukas; Traub, Francisca Martínez; Xia, Chuying Naomi; Molodtsov, Maxim I.; Grama, Abhinav; Yamagata, Masahito; Aharoni, Daniel; Cox, David D.; Golshani, Peyman; Vaziri, Alipasha

doi:10.1038/s41592-018-0008-0

Cited by 178 publications

(154 citation statements)

References 23 publications

Supporting

Mentioning

153

Contrasting

Order By: Relevance

“…While several head-mounted, miniaturized imaging devices have been developed for imaging neural activity in freely moving animals [26][27][28][29][30] , these devices are designed to perform cellular resolution imaging, and thus have small field of view (FOV, < 1 mm 2 ), limiting their application to imaging small brain regions. A head-mounted imaging device with a small FOV has recently been engineered for mesoscale imaging in rats 31 .…”

Section: Introductionmentioning

confidence: 99%

Miniaturized head-mounted device for whole cortex mesoscale imaging in freely behaving mice

Rynes

Surinach

Linn

et al. 2020

Preprint

View full text Add to dashboard Cite

The advent of genetically encoded calcium indicators, along with surgical preparations such as thinned skulls or refractive index matched skulls, have enabled mesoscale cortical activity imaging in head-fixed mice. Such imaging studies have revealed complex patterns of coordinated activity across the cortex during spontaneous behaviors, goal-directed behavior, locomotion, motor learning, and perceptual decision making. However, neural activity during unrestrained behavior significantly differs from neural activity in head-fixed animals. Whole-cortex imaging in freely behaving mice will enable the study of neural activity in a larger, more complex repertoire of behaviors not possible in head-fixed animals. Here we present the “Mesoscope,” a wide-field miniaturized, head-mounted fluorescence microscope compatible with transparent polymer skulls recently developed by our group. With a field of view of 8 mm x 10 mm and weighing less than 4 g, the Mesoscope can image most of the mouse dorsal cortex with resolution ranging from 39 to 56 µm. Stroboscopic illumination with blue and green LEDs allows for the measurement of both fluorescence changes due to calcium activity and reflectance signals to capture hemodynamic changes. We have used the Mesoscope to successfully record mesoscale calcium activity across the dorsal cortex during sensory-evoked stimuli, open field behaviors, and social interactions. Finally, combining the mesoscale imaging with electrophysiology enabled us to measure dynamics in extracellular glutamate release in the cortex during the transition from wakefulness to natural sleep.

show abstract

Section: Introductionmentioning

confidence: 99%

Miniaturized head-mounted device for whole cortex mesoscale imaging in freely behaving mice

Rynes

Surinach

Linn

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…However, it is impossible to eliminate the time lag for scanning multiple areas in the large field. A wearable endoscope equipped with a light-field optics 23 provides 3D imaging with no time lag but with sacrifice of spatial resolution, but it requires a large amount of calculation for the reconstruction of 3D image. On the other hand, a method of 3D imaging by combining a light-field or HiLo (highly inclined and laminated optical sheet) microscopy with a stage system that keeps up with fast motion of an animal was developed 24,25 .…”

Section: Discussionmentioning

confidence: 99%

Wide and Deep Imaging of Neuronal Activities by a Wearable NeuroImager Reveals Premotor Activity in the Whole Motor Cortex

Kobayashi

Islam

Sato

et al. 2018

Preprint

View full text Add to dashboard Cite

SummaryWearable technologies for functional whole brain imaging in freely moving animals would advance our understanding of cognitive processing and adaptive behavior. Fluorescence imaging can visualize the activity of individual neurons in real time, but conventional microscopes have limited sample coverage in both the width and depth of view. Here we developed a novel head-mounted laser camera (HLC) with macro and deep-focus lenses that enable fluorescence imaging at cellular resolution for comprehensive imaging in mice expressing a layer- and cell type-specific calcium probe. We visualized orientation selectivity in individual excitatory neurons across the whole visual cortex of one hemisphere, and cell assembly expressing the premotor activity that precedes voluntary movement across the motor cortex of both hemispheres. Including options for multiplex and wireless interfaces, our wearable, wide- and deep-imaging HLC technology could enable simple and economical mapping of neuronal populations underlying cognition and behavior.

show abstract

“…Additionally, sophisticated computational approaches have allowed for disentangling the complex behavioral expressions of animals into patterns of reoccurring modules [3][4][5] . In vivo single unit recording 6 , along with recent advances in in vivo voltage imaging 7 and miniaturized calcium imaging techniques [8][9][10] , facilitate realtime measurements of neuronal activity in freely moving animals. Together, these techniques provide a platform for correlating recorded neuronal activity and behavior.…”

Section: Introductionmentioning

confidence: 99%

DeepLabStream: Closing the loop using deep learning-based markerless, real-time posture detection

Schweihoff

Loshakov

Kück

et al. 2019

Preprint

View full text Add to dashboard Cite

In general, animal behavior can be described as the neuronal-driven sequence of reoccurring postures through time. Current technologies enable offline pose estimation with high spatio-temporal resolution, however to understand complex behaviors, it is necessary to correlate the behavior with neuronal activity in real-time. Here we present DeepLabStream, a highly versatile, closed-loop solution for freely moving mice that can autonomously conduct behavioral experiments ranging from behavior-based learning tasks to posture-dependent optogenetic stimulation. DeepLabStream has a temporal resolution in the millisecond range, can operate with multiple devices and can be easily tailored to a wide range of species and experimental designs. We employ DeepLabStream to autonomously run a second-order olfactory conditioning task for freely moving mice and to deliver optogenetic stimuli based on mouse head-direction.

show abstract

High-speed volumetric imaging of neuronal activity in freely moving rodents

Cited by 178 publications

References 23 publications

Miniaturized head-mounted device for whole cortex mesoscale imaging in freely behaving mice

Miniaturized head-mounted device for whole cortex mesoscale imaging in freely behaving mice

Wide and Deep Imaging of Neuronal Activities by a Wearable NeuroImager Reveals Premotor Activity in the Whole Motor Cortex

DeepLabStream: Closing the loop using deep learning-based markerless, real-time posture detection

Contact Info

Product

Resources

About