High-speed transverse and axial optical force measurements using amplitude filter masks

Kashchuk, Anatolii V.; Nieminen, Timo A.; Rubinsztein‐Dunlop, Halina; Stilgoe, Alexander B.

doi:10.1364/oe.27.010034

Cited by 8 publications

(8 citation statements)

References 26 publications

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“…15,16 Armstrong et al 16 combined the use of a PSD with the use of position-sensitive masked detection (PSMD), selectively reflecting light in different directions using an appropriately defined mask (details about the mask can be found in Ref. 17), to perform full three-dimensional (3D) direct optical force measurements of trapped single Escherichia coli (E. coli) cells. Figures 2(a) and 2(b) show the schematic of a trapped motile E. coli and the 3D representation of optical force contributions, respectively.…”

Section: Advances For Quantitative Force Measurements In Biological Systemsmentioning

confidence: 99%

Optical manipulation: advances for biophotonics in the 21st century

Corsetti

Dholakia

2021

J. Biomed. Opt.

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Significance: Optical trapping is a technique capable of applying minute forces that has been applied to studies spanning single molecules up to microorganisms.Aim: The goal of this perspective is to highlight some of the main advances in the last decade in this field that are pertinent for a biomedical audience.Approach: First, the direct determination of forces in optical tweezers and the combination of optical and acoustic traps, which allows studies across different length scales, are discussed. Then, a review of the progress made in the direct trapping of both single-molecules, and even single-viruses, and single cells with optical forces is outlined. Lastly, future directions for this methodology in biophotonics are discussed.Results: In the 21st century, optical manipulation has expanded its unique capabilities, enabling not only a more detailed study of single molecules and single cells but also of more complex living systems, giving us further insights into important biological activities.Conclusions: Optical forces have played a large role in the biomedical landscape leading to exceptional new biological breakthroughs. The continuous advances in the world of optical trapping will certainly lead to further exploitation, including exciting in-vivo experiments.

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Section: Advances For Quantitative Force Measurements In Biological Systemsmentioning

confidence: 99%

Optical manipulation: advances for biophotonics in the 21st century

Corsetti

Dholakia

2021

J. Biomed. Opt.

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“…In our experiment, we trap motile E. coli using HOT; the setup has been previously described in (Kashchuk, Nieminen, Rubinsztein-Dunlop and Stilgoe, 2019), and here we only give a brief summary. We use a 1064 nm fiber laser (YLR-10-1064-LP, 10W, IPG Photonics) focused by a high numerical aperture objective (Olympus UPlanSApo 60×, water immersion, 1.2 NA).…”

Section: Orientating Motile E Coli With Dynamic Potentialsmentioning

confidence: 99%

Orientation of swimming cells with annular beam optical tweezers

Lenton

Armstrong

Stilgoe

et al. 2020

Optics Communications

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A R T I C L E I N F OKeywords: optical trapping dual beam trap annular beams spatial light modulator dynamic orientation motile particles A B S T R A C TOptical tweezers are a versatile tool that can be used to manipulate small particles including both motile and non-motile bacteria and cells. The orientation of a non-spherical particle within a beam depends on the shape of the particle and the shape of the light field. By using multiple beams, sculpted light fields or dynamically changing beams, it is possible to control the orientation of certain particles. In this paper we discuss the orientation of the rod-shaped bacteria Escherichia coli (E. coli) using dynamically shifting annular beam optical tweezers. We begin with examples of different beams used for the orientation of rod-shaped particles. We discuss the differences between orientation of motile and non-motile particles, and explore annular beams and the circumstances when they may be beneficial for manipulation of non-spherical particles or cells. Using simulations we map out the trajectory the E. coli takes. Estimating the trap stiffness along the trajectory gives us an insight into how stable an intermediate rotation is with respect to the desired orientation. Using this method, we predict and experimentally verify the change in the orientation of motile E. coli from vertical to near-horizontal with only one intermediate step.The method is not specific to exploring the orientation of particles and could be easily extended to quantify the stability of an arbitrary particle trajectory.

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“…We used an optical tweezers setup to trap cells and carry out three-dimensional direct force measurements. This setup and the force measurement technique have been described in detail [14]. Here we provide a brief summary.…”

Section: Apparatusmentioning

confidence: 99%

“…Through Abbe's sine condition [22], a change in the position of the beam's center of mass, which is tracked by a suitable detector, will correspond to a well-defined deflection angle which is used to determine the force a particle is experiencing. Force measurements in the focal plane are carried out using a PSD, and measurements in the axial direction are obtained with a balanced photodiode detector, following the methods detailed in [14].…”

Section: Determining Optical Forcesmentioning

confidence: 99%

“…Optical tweezers can be used to trap cells directly without attaching probe particles or markers which might otherwise change the dynamics of the cell. By using optical tweezers in conjunction with position sensitive detectors (PSDs) or quadrant photodiodes (QPDs), the optical force and consequently the cell motility force can be directly measured, [14,15].…”

Section: Introductionmentioning

confidence: 99%

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Optical Force Measurements Illuminate Dynamics of Escherichia coli in Viscous Media

et al. 2020

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Escherichia coli and many other bacteria swim through media with the use of flagella, which are deformable helical propellers. When the viscosity of media is increased, a peculiar phenomenon can be observed in which the organism's motility appears to improve. This improvement in the cell's swimming speed has previously been explained by modified versions of resistive force theory (RFT) which accounts for the interaction between flagella and molecules associated with the viscosity increase. Using optical tweezers, we measure the swimming force of individual E. coli in solutions of varying viscosity. By using probe-free force measurements, we are able to quantitatively validate and compare RFT and proposed modifications to the theory. We find that the force produced by the flagellum remains relatively constant even when the viscosity of the medium increases by approximately two orders of magnitude, contrary to predictions of RFT and variants. We conclude that the observed swimming forces can be explained by allowing the flagella geometry to deform as the viscosity of the surrounding medium is increased.

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High-speed transverse and axial optical force measurements using amplitude filter masks

Cited by 8 publications

References 26 publications

Optical manipulation: advances for biophotonics in the 21st century

Optical manipulation: advances for biophotonics in the 21st century

Orientation of swimming cells with annular beam optical tweezers

Optical Force Measurements Illuminate Dynamics of Escherichia coli in Viscous Media

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