High-Speed Scanning Electrochemical Microscopy Method for Substrate Kinetic Determination: Application to Live Cell Imaging in Human Cancer

Kuss, Sabine; Trinh, Dao; Mauzeroll, Janine

doi:10.1021/acs.analchem.5b01269

Cited by 32 publications

(35 citation statements)

References 43 publications

(73 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Another main limitation is the relatively low temporal resolution, since the in situ detection of MIC is a time-dependent process. Improving the high-speed SECM mode is certainly another direction for future progress in biological systems, especially for an in situ MIC study [180,181]. …”

Section: Applicationsmentioning

confidence: 99%

Recent Advances in Scanning Electrochemical Microscopy for Biological Applications

Huang

Lou

et al. 2018

Materials

View full text Add to dashboard Cite

Scanning electrochemical microscopy (SECM) is a chemical microscopy technique with high spatial resolution for imaging sample topography and mapping specific chemical species in liquid environments. With the development of smaller, more sensitive ultramicroelectrodes (UMEs) and more precise computer-controlled measurements, SECM has been widely used to study biological systems over the past three decades. Recent methodological breakthroughs have popularized SECM as a tool for investigating molecular-level chemical reactions. The most common applications include monitoring and analyzing the biological processes associated with enzymatic activity and DNA, and the physiological activity of living cells and other microorganisms. The present article first introduces the basic principles of SECM, followed by an updated review of the applications of SECM in biological studies on enzymes, DNA, proteins, and living cells. Particularly, the potential of SECM for investigating bacterial and biofilm activities is discussed.

show abstract

Section: Applicationsmentioning

confidence: 99%

Recent Advances in Scanning Electrochemical Microscopy for Biological Applications

Huang

Lou

et al. 2018

Materials

View full text Add to dashboard Cite

show abstract

“…For SECM, the challenge of high-speed imaging remains the availability of models that take into account the effects of increased and forced convection, fluid flow and changes to diffusion, caused by the probe moving at increased velocities [120][121][122].…”

Section: Fast Scanning and Imaging Movies Obtained With Sepmmentioning

confidence: 99%

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Lazenby

White

2018

Chemosensors

View full text Add to dashboard Cite

This review discusses a broad range of recent advances (2013)(2014)(2015)(2016)(2017) in chemical imaging using electrochemical methods, with a particular focus on techniques that have been applied to study cellular processes, or techniques that show promise for use in this field in the future. Non-scanning techniques such as microelectrode arrays (MEAs) offer high time-resolution (<10 ms) imaging; however, at reduced spatial resolution. In contrast, scanning electrochemical probe microscopies (SEPMs) offer higher spatial resolution (as low as a few nm per pixel) imaging, with images collected typically over many minutes. Recent significant research efforts to improve the spatial resolution of SEPMs using nanoscale probes and to improve the temporal resolution using fast scanning have resulted in movie (multiple frame) imaging with frame rates as low as a few seconds per image. Many SEPM techniques lack chemical specificity or have poor selectivity (defined by the choice of applied potential for redox-active species). This can be improved using multifunctional probes, ion-selective electrodes and tip-integrated biosensors, although additional effort may be required to preserve sensor performance after miniaturization of these probes. We discuss advances to the field of electrochemical imaging, and technological developments which are anticipated to extend the range of processes that can be studied. This includes imaging cellular processes with increased sensor selectivity and at much improved spatiotemporal resolution than has been previously customary.

show abstract

“…Mauzeroll and coworkers, used probe speeds in SECM of 50 µm s -1 to scan linescans of a single HeLa cell [110]. For SECM, the challenge of high-speed imaging remains the availability of models that take into account the effects of increased and forced convection, fluid flow and changes to diffusion, caused by the probe moving at increased velocities [111][110] [112].…”

Section: Fast Scanning and Imaging Movies Obtained With Sepmmentioning

confidence: 99%

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Lazenby¹,

White²

2018

Preprint

View full text Add to dashboard Cite

High-Speed Scanning Electrochemical Microscopy Method for Substrate Kinetic Determination: Application to Live Cell Imaging in Human Cancer

Cited by 32 publications

References 43 publications

Recent Advances in Scanning Electrochemical Microscopy for Biological Applications

Recent Advances in Scanning Electrochemical Microscopy for Biological Applications

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Advances and Perspectives in Chemical Imaging in Cellular Environments Using Electrochemical Methods

Contact Info

Product

Resources

About