High-speed gradient separations of peptides and proteins using polymer-monolithic poly(styrene-co-divinylbenzene) capillary columns at ultra-high pressure

Vaast, Axel; Nováková, Lucie; Desmet, Gert; Haan, Björn de; Swart, Remco; Eeltink, Sebastiaan

doi:10.1016/j.chroma.2013.07.040

Cited by 28 publications

(27 citation statements)

References 28 publications

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“…longer column). 69 This kind of result shows high performance separation at ultrahigh pressure can be carried out using a commercially available polymer monolith column, though the degree of retention of small organic molecules must be considered when we apply the column to food or environmental analysis. Still, although the examples of the application of polymer monolith columns for this purpose are limited, there are several results, and they will be introduced in the next section.…”

Section: Features Of Organic Polymer Monolithic Columnsmentioning

confidence: 91%

Monolithic Columns in Fast Liquid Chromatography

Hara¹,

Núñez²,

Ikegami³

et al. 2015

Fast Liquid Chromatography–Mass Spectrometry Methods in Food and Environmental Analysis

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Section: Features Of Organic Polymer Monolithic Columnsmentioning

confidence: 91%

Monolithic Columns in Fast Liquid Chromatography

Hara¹,

Núñez²,

Ikegami³

et al. 2015

Fast Liquid Chromatography–Mass Spectrometry Methods in Food and Environmental Analysis

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“…The meso‐ (2–50 nm) and micropore (<2 nm) distribution determined via argon adsorption isotherms displayed in Figure D shows that the monolith contains a small fraction of pores <10 nm . It has been demonstrated that capillary column formats (≤200 μm id) are pressure stable at UHPLC conditions due to the establishment of covalent bonds between the monolith and the column wall after surface modification .…”

Section: Characteristics Of Conventional Polymer–monolithic Stationarmentioning

confidence: 96%

Guidelines for tuning the macropore structure of monolithic columns for high‐performance liquid chromatography

Dores-Sousa

Fernández-Pumarega

Vos

et al. 2018

J of Separation Science

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The ability to control the external porosity and to tune the dimensions of the macropore size on multiple length scales provides the possibility of tailoring the monolithic support structure towards separation performance. This paper discusses the properties of conventional polymer–monolithic stationary phases and its limitations regarding the effects of morphology on kinetic performance. Furthermore, guidelines to improve the macropore structure are discussed. The optimal monolithic macropore structure is characterized by high external porosity (while maintaining ultra‐high‐pressure stability), high structure homogeneity, polymer globule clusters in the submicron range, and macropores with a diameter tuned toward speed (small diameter in the 100–500 nm range using short beds) or efficiency (larger macropores in the range of 500 nm–1 μm allowing the use of longer column formats). Finally, promising approaches to control the morphology are discussed.

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“…removal of organic pollutants, removal of toxic metals, ions exchange processes), [1][2][3][4][5] hydrogen storage, 6,7 hydrometallurgy, 8, chromatography 9,10 and separation of biomolecules. 11,12 In addition, the utilization of P(S-DVB) resins as catalyst in a number of industrial processes is also quite important, as they offer potentially much higher capacity for the supported functionality than the conventional inorganic carriers. [13][14][15][16][17][18][19] Thus, many key large-scale chemical processes have been established employing sulfonic acid resins as catalyst.…”

Section: Introductionmentioning

confidence: 99%

Atomistic simulations of the structure of highly crosslinked sulfonated poly(styrene-co-divinylbenzene) ion exchange resins

et al. 2015

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The microscopic structure of highly crosslinked sulfonated poly(styrene-codivinylbenze) resins have been modeled by generating atomistic microstructures using stochastic-like algorithms that are , subsequently, relaxed using molecular dynamics.Two different generation algorithms have been tested. Relaxation of the microstructures generated by the first algorithm, which is based on a homogeneous construction of the resin, leads to a significant overestimation of the experimental density as well as to an unsatisfactory description of the porosity. In contrast, the generation approach that combines algorithms for the heterogeneous growing and branching of the chains enables the formation of crosslinks with different topologies. In particular, the intrinsic heterogeneity observed in these resins is well reproduced when topological loops, which are defined by two or more crosslinks closing a cycle, are present in their microscopic description. Thus, the apparent density, porosity and pore volume estimated using microstructures with these topological loops, called super-crosslinks, are in very good agreement with experimental measures. Although the backbone dihedral angle distribution of the generated and relaxed models is not influenced by the topology, the number and type of crosslinks affect the medium-and long-range atomic disposition of the backbone atoms and the distribution of the sulfonic groups. Analysis of the distribution of the local density indicates that super-crosslinks are responsible of the heterogeneous homogenization observed during the MD relaxation. Finally, - stacking interactions between aromatic phenyl groups have been analyzed, those in which the two rings adopt a T-shaped disposition being considerably more abundant than those with the rings co-facially oriented, independently of the resin topology.3

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High-speed gradient separations of peptides and proteins using polymer-monolithic poly(styrene-co-divinylbenzene) capillary columns at ultra-high pressure

Cited by 28 publications

References 28 publications

Monolithic Columns in Fast Liquid Chromatography

Monolithic Columns in Fast Liquid Chromatography

Guidelines for tuning the macropore structure of monolithic columns for high‐performance liquid chromatography

Atomistic simulations of the structure of highly crosslinked sulfonated poly(styrene-co-divinylbenzene) ion exchange resins

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