High speed capillary zone electrophoresis with laser induced fluorescence detection

Nickerson, Beverly; Jorgenson, James W.

doi:10.1002/jhrc.1240110708

Cited by 53 publications

(19 citation statements)

References 6 publications

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“…In spite of being a less-than-ideal spectral match for the helium-cadmium laser, impressive sensitivities were recently shown in fast CZE with the NDA-derivatized amino acids (38); however, the electropherogram contained only a few standard amino acids. As an alternative, Oates and Jorgenson adequately resolved all amino acid NDA derivatives using open tubular LC with electrochemical detection (39).…”

Section: Microcolumn Separations Of Proteins and Their Degradation Prmentioning

confidence: 99%

Recent advances in the isolation and structural studies of biomacromolecules using microcolumn techniques

Novotný

1990

J Microcolumn Separations

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Abstract. Microcolumn liquid chromatography and capillary electrophoresis facilitate isolation of biological macromolecules at subnanogram levels. In order to characterize structurally such small amounts of labile materials, it is essential to develop new nanoscale sample treatment approaches and highly sensitive analytical techniques for the determination of biomolecular fragments. Progress in this area is summarized, using selected examples of the amino acid, peptide, and oligonucleotide separations.

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Section: Microcolumn Separations Of Proteins and Their Degradation Prmentioning

confidence: 99%

Recent advances in the isolation and structural studies of biomacromolecules using microcolumn techniques

Novotný

1990

J Microcolumn Separations

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“…By far the most frequently applied derivatization reagent for primary amines is o-phthaldialdehyde (OPA) [22,36,37,39,46,[58][59][60][61]. Dialdehydes or aroylaldehydes react relatively fast with primary amines, resulting in highly fluorescent isoindoles (Fig.…”

Section: Isoindole Derivativesmentioning

confidence: 99%

“…The only problem in using OPA is the limited stability of some of its derivatives because of oxidation andlor photodecomposition. Although the choice of the thiol is of some influence on the stability, another fluorogenic reagent, naphthalene-2,3-dicarboxyaldehyde (NDA), can be used to improve the stability [37,46,[53][54][55][56][57]]. An additional advantage of NDA over OPA is that a longer excitation wavelength must be used, resulting in an improved signal-to-noise ratio for the derivatives.…”

Section: Isoindole Derivativesmentioning

confidence: 99%

Pre‐, on‐ and post‐column derivatization in capillary electrophoresis

et al. 1997

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This survey gives a short overview of the various reagents and procedures that can be used for pre-, post- and on-column derivatization in capillary electrophoresis. First there is an introduction about capillary electrophoresis as an analytical technique; this is followed by a discussion of the pros and cons of the various modes of derivatization and a comparison with liquid chromatography. In the following paragraphs the reagents for a number of functional groups are discussed. The emphasis is on derivatization of the amino group. Most of the information on the reagents and derivatization procedures is listed in tables together with information on the detection mode, analytes, sensitivity and samples. In addition to the amino group, information is given on labeling of aldehyde, keto, carboxyl, hydroxyl and sulfhydryl groups.

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“…Even though a significant increase in both sensitivity and selectivity can be obtained, an important limitation of such advanced detection methods is, unfortunately, that only a limited number of compounds show native fluorescence or are electrochemically active, while chemiluminescence is only suitable for an even more limited group of analytes. Of course, these problems can (partly) be solved by introducing chemical derivatization procedures either prior to [2], during [3] or after [4,5] the separation or by using indirect detection methods [6]. However, when following one of these approaches new problems may be encountered because trace amounts of analytes often cannot be derivatized quantitatively and additional chemical noise will result in an increased background.…”

Section: Introductionmentioning

confidence: 96%

On-line chromatographic pretreatment of samples with varying salt concentrations for capillary electrophoresis

et al. 1997

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SummaryVarious methods are currently under investigation to improve concentration detection limits in capillary electrophoresis (CE). Stacking and isotachophoresis coupled in-line with free-solution electrophoresis are the techniques most frequently used. Samples containing a high and/or varying salt concentration, such as urine and serum, are difficult to handle: usually a significant loss in electrophoretic efficiency is observed compared with samples possessing a low electric conductivity. In the present paper an alternative approach is developed, i.e. a liquid chromatographic (LC)-type of sample pretreatment is coupled on-line with CE. To demonstrate the feasibility of this approach, the separation of three model compounds (benzoates) in water containing up to 400 mM of sodium chloride is studied using a 50 mM borate CE buffer of pH 9.5. The direct injection of samples with high salt concentrations in CE results in peak splitting and/or serious band broadening. These problems are not encountered when using the present LC-CE system. In addition, the detection limits are hardly influenced by the salt concentration of the sample; this underlines the robustness of the system.

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High speed capillary zone electrophoresis with laser induced fluorescence detection

Cited by 53 publications

References 6 publications

Recent advances in the isolation and structural studies of biomacromolecules using microcolumn techniques

Recent advances in the isolation and structural studies of biomacromolecules using microcolumn techniques

Pre‐, on‐ and post‐column derivatization in capillary electrophoresis

On-line chromatographic pretreatment of samples with varying salt concentrations for capillary electrophoresis

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