High speed 3D overhauser‐enhanced MRI using combined b‐SSFP and compressed sensing

Sarracanie, Mathieu; Armstrong, Brandon D.; Stockmann, Jason P.; Rosen, Matthew S.

doi:10.1002/mrm.24705

Cited by 44 publications

(60 citation statements)

References 53 publications

(68 reference statements)

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“…[23] In situ and ex situ MR molecular images presented in this study were obtained with <2% hyperpolarized agent; achieving higher levels of hyperpolarization in combination with improved pulse sequences (including 3D) and compressed sensing [24] may provide significantly better spatial and temporal resolution.…”

Section: Resultsmentioning

confidence: 99%

“…Ex situ MRI of a liquid solution after exchange with p -H 2 was successfully performed at 47.5 mT with sub-millimeter in-plane spatial resolution (pixel size 125 × 125 µm 2 ) in a few seconds; this demonstrated the feasibility of low-field parahydrogen-based hyperpolarization detection with ultra-high spatial and temporal resolution. Ultrafast 3D MRI of HP gases was demonstrated, [8b] and ultrafast 3D low-field MRI [24] can in principle be applied to SABRE hyperpolarized contrast agents leading to 3D MRI images with sub-millimeter voxel size, because sufficient NMR sensitivity was already demonstrated, as seen in Figure 3 e with an effective voxel size of <0.5 mm 3 . Furthermore, in situ low-field NMR and MRI were also employed for imaging of the product (pyridine) and intermediates (iridium dihydride) of hydrogen exchange during hydrogen gas bubbling though the solution.…”

Section: Discussionmentioning

confidence: 99%

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In Situ and Ex Situ Low‐Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization

et al. 2014

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By using 5.75 and 47.5 mT nuclear magnetic resonance (NMR) spectroscopy, up to 105-fold sensitivity enhancement through signal amplification by reversible exchange (SABRE) was enabled, and subsecond temporal resolution was used to monitor an exchange reaction that resulted in the buildup and decay of hyperpolarized species after parahydrogen bubbling. We demonstrated the high-resolution low-field proton magnetic resonance imaging (MRI) of pyridine in a 47.5 mT magnetic field endowed by SABRE. Molecular imaging (i.e. imaging of dilute hyperpolarized substances rather than the bulk medium) was conducted in two regimes: in situ real-time MRI of the reaction mixture (in which pyridine was hyperpolarized), and ex situ MRI (in which hyperpolarization decays) of the liquid hyperpolarized product. Low-field (milli-Tesla range, e.g. 5.75 and 47.5 mT used in this study) parahydrogen-enhanced NMR and MRI, which are free from the limitations of high-field magnetic resonance (including susceptibility-induced gradients of the static magnetic field at phase interfaces), potentially enables new imaging applications as well as differentiation of hyperpolarized chemical species on demand by exploiting spin manipulations with static and alternating magnetic fields.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In Situ and Ex Situ Low‐Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization

et al. 2014

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“…In order to measure ROS, several methods have been developed including optical redox scanning 15 and exogenous contrast magnetic resonance imaging (MRI)-based methods. [16][17][18][19][20][21] Optical redox scanning acquires ex vivo fluorescence images from endogenous reduced nicotinamide adenine dinucleotide (NADH) and oxidized flavoproteins (Fp) in order to map the tissue redox state that is related to ROS-induced oxidative stress. 15 However, this technique is invasive and only applicable to ex vivo tissues.…”

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confidence: 99%

Imaging short‐lived reactive oxygen species (ROS) with endogenous contrast MRI

Tain

Scotti

et al. 2017

Magnetic Resonance Imaging

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Purpose: To characterize the relaxation properties of reactive oxygen species (ROS) for the development of endogenous ROS contrast magnetic resonance imaging (MRI). Materials and Methods: ROS-producing phantoms and animal models were imaged at 9.4T MRI to obtain T 1 and T 2 maps. Egg white samples treated with varied concentrations of hydrogen peroxide (H 2 O 2 ) were used to evaluate the effect of produced ROS in T 1 and T 2 for up to 4 hours. pH and temperature changes due to H 2 O 2 treatment in egg white were also monitored. The influences from H 2 O 2 itself and oxygen were evaluated in bovine serum albumin (BSA) solution producing no ROS. In addition, dynamic temporal changes of T 1 in H 2 O 2 -treated egg white samples were used to estimate ROS concentration over time and hence the detection sensitivity of relaxation-based endogenous ROS MRI. The relaxivity of ROS was compared with that of Gd-DTPA as a reference. Finally, the feasibility of in vivo ROS MRI with T 1 mapping acquired using an inversion recovery sequence was demonstrated with a well-established rotenone-treated mouse model (n 5 6). Results: pH and temperature changes in treated egg white samples were insignificant (<0.1 unit and <18C, respectively). T 1 relaxation time in the H 2 O 2 -treated egg white was reduced significantly (P < 0.05), while there was only small reduction in T 2 (<10%). In the H 2 O 2 -treated BSA solution that produce no ROS, there was a small change in T 1 due to H 2 O 2 itself (61%), although a significant T 2 -shortening effect was observed (>10%, P < 0.05). Also, there was a small reduction in T 1 (13 6 1%) and T 2 (1 6 2%) from molecular oxygen. The detection sensitivity of ROS MRI was estimated around 10 pM. The T 1 relaxivity of ROS was found to be much higher than that of Gd-DTPA (3.4 3 10 7 vs. 0.9 s 21 ÁmM 21 ). Finally, significantly reduced T 1 was observed in rotenone-treated mouse brain (5.1 6 2.5%, P < 0.05). Conclusion: We demonstrated in the study that endogenous ROS MRI based on the paramagnetic effect has sensitivity for in vitro and in vivo applications. Level of Evidence: 2 Technical Efficacy Stage: 2 J. MAGN. RESON. IMAGING 2018;47:222-229. R eactive oxygen species (ROS), including singlet oxygen, and hydroxyl radical (ÁOH), have long been subjects of study due to their central role in cell signaling, the aging process, and in the pathogenesis of a variety of diseases such as cardiovascular diseases, diabetes, cancer, Alzheimer's, and Parkinson's diseases. 1-5 ROS can be divided into radical species (eg, ÁOH) and nonradical species (eg, H 2 O 2 ). They are mainly produced by several different enzyme systems (eg, cytosolic enzyme) and by the mitochondrial complex I and III. 6 They can react readily with any surrounding tissue component (eg, lipids, proteins, and DNA) 2 and can initiate complicated chain processes with high biological impact (eg, Haber-Weiss or Fenton reactions). 7 The high reactivity and short lifetime of radical ROS make them very challenging to detect. For example, the lifetime...

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“…Furthermore, the use of compressed sensing in conjunction with faster and more sensitive imaging sequences 30 can further improve the detection sensitivity and accelerate total imaging time.…”

Section: Resultsmentioning

confidence: 99%

High-Resolution Low-Field Molecular Magnetic Resonance Imaging of Hyperpolarized Liquids

et al. 2014

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We demonstrate the feasibility of microscale molecular imaging using hyperpolarized proton and carbon-13 MRI contrast media and low-field (47.5 mT) preclinical scale (38 mm i.d.) 2D magnetic resonance imaging (MRI). Hyperpolarized proton images with 94 × 94 μm2 spatial resolution and hyperpolarized carbon-13 images with 250 × 250 μm2 in-plane spatial resolution were recorded in 4–8 s (largely limited by the electronics response), surpassing the in-plane spatial resolution (i.e., pixel size) achievable with micro-positron emission tomography (PET). These hyperpolarized proton and 13C images were recorded using large imaging matrices of up to 256 × 256 pixels and relatively large fields of view of up to 6.4 × 6.4 cm2. 13C images were recorded using hyperpolarized 1-13C-succinate-d2 (30 mM in water, %P13C = 25.8 ± 5.1% (when produced) and %P13C = 14.2 ± 0.7% (when imaged), T1 = 74 ± 3 s), and proton images were recorded using 1H hyperpolarized pyridine (100 mM in methanol-d4, %PH = 0.1 ± 0.02% (when imaged), T1 = 11 ± 0.1 s). Both contrast agents were hyperpolarized using parahydrogen (>90% para-fraction) in an automated 5.75 mT parahydrogen induced polarization (PHIP) hyperpolarizer. A magnetized path was demonstrated for successful transportation of a 13C hyperpolarized contrast agent (1-13C-succinate-d2, sensitive to fast depolarization when at the Earth’s magnetic field) from the PHIP polarizer to the 47.5 mT low-field MRI. While future polarizing and low-field MRI hardware and imaging sequence developments can further improve the low-field detection sensitivity, the current results demonstrate that microscale molecular imaging in vivo is already feasible at low (<50 mT) fields and potentially at low (∼1 mM) metabolite concentrations.

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High speed 3D overhauser‐enhanced MRI using combined b‐SSFP and compressed sensing

Cited by 44 publications

References 53 publications

In Situ and Ex Situ Low‐Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization

In Situ and Ex Situ Low‐Field NMR Spectroscopy and MRI Endowed by SABRE Hyperpolarization

Imaging short‐lived reactive oxygen species (ROS) with endogenous contrast MRI

High-Resolution Low-Field Molecular Magnetic Resonance Imaging of Hyperpolarized Liquids

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