High Specific DNAzyme-Aptamer Sensor for Salmonella paratyphi A Using Single-Walled Nanotubes–Based Dual Fluorescence-Spectrophotometric Methods

Ning, Yi; Li, Wenkai; Duan, Yingfen; Yang, Ming; Deng, Le

doi:10.1177/1087057114528538

Cited by 19 publications

(7 citation statements)

References 31 publications

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“…The performance values obtained in this study (LOD, response time and sensitivity) are comparable to, if not superior to, aptasensors published for the detection of E. coli O157:H7. Most recently, Ning et al (Ning et al, 2015) developed an impedance-based immunosensor for O157:H7 based on self-assembled lectins with a LOD of 100 CFU mL -1 , a linear range from 10 2 to 10 7 CFU mL -1 , and a total detection time of 1 h. Fang et al (Fang et al, 2014) most of the cost derived from the platinum), lightweight, rapid, and reproducible by any biosensing laboratory. The device has a number of potential applications in point of care biosensing, particularly in developing regions where access to conventional fabrication equipment is not likely.…”

Section: Bacteria Detectionmentioning

confidence: 99%

A paper based graphene-nanocauliflower hybrid composite for point of care biosensing

Burrs

Bhargava

Sidhu

et al. 2016

Biosensors and Bioelectronics

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We demonstrate the first report of graphene paper functionalized with fractal platinum nanocauliflower for use in electrochemical biosensing of small molecules (glucose) or detection of pathogenic bacteria (Escherichia coli O157:H7). Raman spectroscopy, scanning electron microscopy and energy dispersive spectroscopy show that graphene oxide-coated nanocellulose was partially reduced by both thermal treatment, and further reduced by chemical treatment (ascorbic acid). Fractal nanoplatinum with cauliflower-like morphology was formed on the reduced graphene oxide paper using pulsed sonoelectrodeposition, producing a conductive paper with an extremely high electroactive surface area (0.29±0.13cm(2)), confirmed by cyclic voltammetry and electrochemical impedance spectroscopy. The platinum surface was functionalized with either glucose oxidase (via chitosan encapsulation) or a RNA aptamer (via covalent linking) for demonstration as a point of care biosensor. The detection limit for both glucose (0.08±0.02μM) and E. coli O157:H7 (≈4 CFUmL(-1)) were competitive with, or superior to, previously reported devices in the biosensing literature. The response time (6s for glucose and 12min for E. coli) were also similar to silicon biochip and commercial electrode sensors. The results demonstrate that the nanocellulose-graphene-nanoplatinum material is an excellent paper-based platform for development of electrochemical biosensors targeting small molecules or whole cells for use in point of care biosensing.

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Section: Bacteria Detectionmentioning

confidence: 99%

A paper based graphene-nanocauliflower hybrid composite for point of care biosensing

Burrs

Bhargava

Sidhu

et al. 2016

Biosensors and Bioelectronics

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“…Those variabilities can be caused by some reasons which are choosing the aptamer pairs, the distance of aptamers from the immobilization zone of membrane, affinity of aptamers [37,38], and experimental assay conditions. Although large numbers of aptamers were recorded in the literature [39][40][41], there is still a lack of their adaptation to LFAs for the recognition of Salmonella species. Because the optimization of test parameters including immobilization procedure of aptamers on capture zones, optimal buffer ingredients, and membrane types, the exact size of GNPs has more complexity than Ab-based strip assays.…”

Section: Antibodies For Lfasmentioning

confidence: 99%

Lateral Flow Assay for Salmonella Detection and Potential Reagents

Çam¹

2020

New Insight Into Brucella Infection and Foodborne Diseases

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Salmonella is among the very important pathogens threating human and animal health. It is a common food pathogen transmitted from animals to humans via contaminated food, drinking water, and air. It invades the intestinal tract of hosts and causes salmonellosis leading to death. S. enteritidis was the most common species accounted for all salmonellosis cases. S. typhimurium is also another significant species causing the serious cases worldwide. To ensure public health, early detection of pathogens is crucial. Lateral flow assay (LFA), immunochromatographic assay, is a simple and rapid diagnostic test kits used in various fields and can be developed by, aptamers, antibodies (Abs), and nucleic acids. They are also being continued to develop different capture reagents coming from the recombinant technology. It has many advantages such as having mature technology, market presence, low cost, easy to use for end users without education, and stable shelf life. Gold nanoparticles (GNPs) are the most commonly used labels in the LFAs for the naked-eye analysis. Therefore, Salmonella detection by LFA based on GNPs in a rapid and simple way is always open to be developed by new reagents and methods.

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“…Applications in food safety control have been reviewed [ 99 ], and in real food situations, e.g., E. coli using a potentiometric aptamer based biosensor with detection of 6 CFU/mL in milk and 25 CFU/mL in apple juice [ 100 ]. Vibrio cholera was detected at 0.85 ng/µL genomic DNA; DNAzyme aptamers for Salmonella paratyphi using nanotubes and fluorescence [ 101 ]. The design of the aptamers was carried out using SELEX (Systematic Evolution of Ligands by Exponential Enrichment).…”

Section: Nucleic Acid Sensorsmentioning

confidence: 99%

Past, Present and Future of Sensors in Food Production

Adley

2014

Foods

113

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Microbial contamination management is a crucial task in the food industry. Undesirable microbial spoilage in a modern food processing plant poses a risk to consumers’ health, causing severe economic losses to the manufacturers and retailers, contributing to wastage of food and a concern to the world’s food supply. The main goal of the quality management is to reduce the time interval between the filling and the detection of a microorganism before release, from several days, to minutes or, at most, hours. This would allow the food company to stop the production, limiting the damage to just a part of the entire batch, with considerable savings in terms of product value, thereby avoiding the utilization of raw materials, packaging and strongly reducing food waste. Sensor systems offer major advantages over current systems as they are versatile and affordable but need to be integrated in the existing processing systems as a process analytical control (PAT) tool. The desire for good selectivity, low cost, portable and usable at working sites, sufficiently rapid to be used at-line or on-line, and no sample preparation devices are required. The application of biosensors in the food industry still has to compete with the standard analytical techniques in terms of cost, performance and reliability.

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High Specific DNAzyme-Aptamer Sensor for Salmonella paratyphi A Using Single-Walled Nanotubes–Based Dual Fluorescence-Spectrophotometric Methods

Cited by 19 publications

References 31 publications

A paper based graphene-nanocauliflower hybrid composite for point of care biosensing

A paper based graphene-nanocauliflower hybrid composite for point of care biosensing

Lateral Flow Assay for Salmonella Detection and Potential Reagents

Past, Present and Future of Sensors in Food Production

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