High Specific Activity Labeling of Protein with I131 by the Iodine Monochloride Method.

Bale, William F.; Helmkamp, R. W.; Davis, Thomas P.; Izzo, Mary Jane; Goodland, Ruth L.; Contreras, M; Spar, Irving L.

doi:10.3181/00379727-122-31148

Cited by 52 publications

(13 citation statements)

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“…After gel filtration on Sephadex G-100, this preparation contained only Fab fragments as determined by double diffusion studies in agar using antisera specific for human Fab or Fc. Radioiodination of the Fab fragments was performed by the iodine monochloride technique (14).…”

Section: Methodsmentioning

confidence: 99%

Neutrophil-binding immunoglobulin G in systemic lupus erythematosus.

Starkebaum¹,

Arend²

1979

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 99%

Neutrophil-binding immunoglobulin G in systemic lupus erythematosus.

Starkebaum¹,

Arend²

1979

J. Clin. Invest.

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“…On admission to the Clinical Research Unit for immunologic evaluation in July, 1965 (at the age of 13 9 / 12 years), chest x-ray showed a pneumonic infiltrate and atelectasis of the right middle lobe; sinus films demonstrated bilateral antral and ethmoid sinus clouding. Absolute lymphocyte counts on two occasions were 2106 and 2592/mm 3 . At bronchoscopy, purulent material was seen in the right middle lobe bronchus.…”

Section: Casementioning

confidence: 99%

“…Gamma globulin fractions from R. B. and K. B., prepared from whole serum by ammonium sulfate fractionation at one-third saturation, were each labelled with m I by the iodine monochloride method of BALE et al [3]; a commercial gamma globulin preparation 5 was labelled with 125 I by the same method. Aliquots containing 50 /J,C were injected intramuscularly.…”

Section: Gamma Globulin Survivalmentioning

confidence: 99%

“…Immediate hypersensitivity was tested by standard scratch and intradermal techniques, using commercial antigens 3 or antigens prepared in the Allergy and Clinical Immunology Laboratory at Duke University Medical Center. Delayed hypersensitivity was assayed using a group of bacterial, viral and fungal antigens, one or more of which usually elicit responses in most normal individuals [11].…”

Section: Skin Testsmentioning

confidence: 99%

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Hereditary Alterations in the Immune Response: Coexistence of ‘Agammaglobulinemia', Acquired Hypogammaglobulinemia and Selective Immunoglobulin Deficiency in a Sibship

Buckley

Sidbury

1968

Pediatr Res

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“…Urines were collected continuously during the time of hospitalization and at intervals thereafter usually coinciding with the plasma samples. The IgG and L-chains were labeled as described by Bale, et al (5). Various preparations of the labeled proteins contained from 0.2 to 0.6 mol of radioiodine per mol of IgG or L-chain.…”

Section: Methodsmentioning

confidence: 99%

The Relationship Between L-Chain Synthesis and γ-Globulin Production

Waterhouse¹,

Abraham²,

Vaughan³

1973

J. Clin. Invest.

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A B S T R A C T The simultaneous administration of labeled human -G globulin and L-chains to subjects has allowed examination of the metabolic relationship between a component part and the whole immunoglobulin molecule. Studies were carried out in a series of control subjects and in a group of patients where substandard production of '-globulin was anticipated, i.e., patients with the nephrotic syndrome on chronic Imuran therapy and patients with uremia. Full expression of the plasma decay curve was obtained for both substances, that for L-chain requiring only 4-5 days except in uremic subjects and that for -y-globulin requiring up to 30-40 days. Urinary excretion of inorganic iodide was also quantitated for 20-30 days. Equilibrium of the extravascular, vascular, and urinary radioactivity from the labeled -yG globulin was usually not seen during this time interval suggesting more than one site of catabolism of the protein.Excess L-chain poduction was about 45% of the total L-chain production and probably in no instance could account for the low e-globulin production seen in certain patients with renal disease.

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High Specific Activity Labeling of Protein with I131 by the Iodine Monochloride Method.

Cited by 52 publications

References 0 publications

Neutrophil-binding immunoglobulin G in systemic lupus erythematosus.

Neutrophil-binding immunoglobulin G in systemic lupus erythematosus.

Hereditary Alterations in the Immune Response: Coexistence of ‘Agammaglobulinemia', Acquired Hypogammaglobulinemia and Selective Immunoglobulin Deficiency in a Sibship

The Relationship Between L-Chain Synthesis and γ-Globulin Production

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