High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Rajamanthrilage, Apeksha C.; Levon, Erin; Uzair, Unaiza; Taylor, C.; Tzeng, Tzuen‐Rong; Anker, Jeffrey N.

doi:10.3791/64252

Cited by 3 publications

(4 citation statements)

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“…In previous studies with X-ray excited luminescence chemical imaging through >1 cm of tissue, we saw no signals that would significantly interfere with our measurements. [25,49] However, if it is a problem for the ultrasound because we are extracting a weaker signal at 2 Hz, one could either try to measure variation in transmittance with external pulse oximeters, or vary the signal to avoid common backgrounds, although the ≈1 s luminescence response time means that increasing the blinking rate would attenuate the modulated signal.…”

Section: Ph Sensor Developmentmentioning

confidence: 99%

Development of pH‐Sensitive Film for Detection of Implant Infection via Ultrasound Luminescent Chemical Imaging

Schober,

Uzair,

Reel

et al. 2024

Advanced Sensor Research

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A new hybrid ultrasound luminescent chemical imaging technique is described along with a pH sensor to image chemical concentrations at the surface of implanted medical devices. The purpose is to detect and study local biochemistry during infection. The sensor comprises a mechanoluminescent film (SrAl2O4:Eu, Dy microphosphors embedded in a biocompatible polymer film) and a pH indicator dye. A focused ultrasound beam generates green luminescence at the ultrasound focal point. By pulsing the ultrasound ON and OFF, the modulated luminescence can be distinguished from persistent luminescence, for high spatial resolution imaging. A red fluorescent dye and the pH indicator dye bromothymol blue are added to the coating to modulate the red‐light transmittance via pH dependent absorbance. Acidosis is observed as an increase in red luminescence intensity in spectroscopy and imaging. The films are sensitive to biologically relevant changes in pH (6.0–8.0) and can be imaged through optically scattering media to mimic tissue. The images have a knife edge spatial resolution of ≈3 mm through optically scattering phantoms, limited by the focused ultrasound spot size. This novel technique may permit the elucidation of implant infection at the implant surface and can be further developed for the measurement of other relevant chemical species in the future.

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Section: Ph Sensor Developmentmentioning

confidence: 99%

Development of pH‐Sensitive Film for Detection of Implant Infection via Ultrasound Luminescent Chemical Imaging

Schober,

Uzair,

Reel

et al. 2024

Advanced Sensor Research

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“…Fortunately, XELCI uses solid implants which will show up on the other imaging techniques, simplifying coregistration; X-ray imaging is especially easy since the XELCI setup already has an X-ray source. 6,7,10 ■ METHODS…”

Section: ■ Introductionmentioning

confidence: 99%

“…X-ray excited luminescent chemical imaging uses a focused X-ray beam to reach deep into the soft tissue without much scattering and produce a luminescent spot from an X-ray scintillator film coated on a medical implant. − The luminescence intensity is modulated by optical absorption from a chemical sensing film on the device. In principle, anything that absorbs light or alters the spectrum can be detected including pH indicators, silver/gold nanoparticles, different types of phosphors, and cut paper resolution targets used herein, and the approach can be generalized to a variety of spectrochemical and mechanical sensing techniques.…”

Section: Introductionmentioning

confidence: 99%

“…That said, each technique has its advantages, and combining multiple image modalities can provide complementary anatomical and contrast-related information. Fortunately, XELCI uses solid implants which will show up on the other imaging techniques, simplifying coregistration; X-ray imaging is especially easy since the XELCI setup already has an X-ray source. ,, …”

Section: Introductionmentioning

confidence: 99%

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Spatial Resolution for X-ray Excited Luminescence Chemical Imaging (XELCI)

Rajamanthrilage,

Uzair,

Millhouse

et al. 2024

Chemical & Biomedical Imaging

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Measuring chemical concentrations at the surface of implanted medical devices is important for elucidating the local biochemical environment, especially during implant infection. Although chemical indicator dyes enable chemical measurements in vitro, they are usually ineffective when measuring through tissue because the background obscures the dye signal and scattering dramatically reduces the spatial resolution. X-ray excited luminescent chemical imaging (XELCI) is a recent imaging modality which overcomes these limitations using a focused X-ray beam to excite a small spot of red light on scintillator-coated medical implants with well-defined location (because X-rays are minimally scattered) and low background. A spectrochemical indicator film placed over the scintillator layer, e.g., a polymer film containing pH-indicator dyes, absorbs some of the luminescence according to the local chemical environment, and this absorption is then detected by measuring the light intensity/spectrum passing through the tissue. A focused X-ray beam is used to scan point-by-point with a spatial resolution mainly limited by the X-ray beam width with minimum increase from X-ray absorption and scattering in the tissue. X-ray resolution, implant surface specificity, and chemical sensitivity are the three key features of XELCI. Here, we study spatial resolution using optically absorptive targets. For imaging a series of lines, the 20−80% knife-edge resolution was ∼285 (±15) μm with no tissue and 475 ± 18 and 520 ± 34 μm, respectively, through 5 and 10 mm thick tissue. Thus, doubling the tissue depth did not appreciably change the spatial resolution recorded through the tissue. This shows the promise of XELCI for submillimeter chemical imaging through tissue.

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Medical imaging: A Critical Review on X-ray Imaging for the Detection of Infection

Irede,

Aworinde,

Lekan

et al. 2024

Biomedical Materials & Devices

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High Spatial Resolution Chemical Imaging of Implant-Associated Infections with X-ray Excited Luminescence Chemical Imaging Through Tissue

Cited by 3 publications

References 0 publications

Development of pH‐Sensitive Film for Detection of Implant Infection via Ultrasound Luminescent Chemical Imaging

Development of pH‐Sensitive Film for Detection of Implant Infection via Ultrasound Luminescent Chemical Imaging

Spatial Resolution for X-ray Excited Luminescence Chemical Imaging (XELCI)

Medical imaging: A Critical Review on X-ray Imaging for the Detection of Infection

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