High Sensitivity Micro-Elastometry: Applications in Blood Coagulopathy

Wu, Gongting; Krebs, C. R.; Lin, Feng; Wolberg, Alisa S.; Oldenburg, Amy L.

doi:10.1007/s10439-013-0817-3

Cited by 19 publications

(29 citation statements)

References 26 publications

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“…Atomic force microscopy-based micro- and nanorheology allows for precise measurements of the clot viscoelastic properties using a very small sample volume in the 3-50 nN force range [25, 26]. Another method named resonant acoustic spectroscopy with optical vibrometry measures the clot elastic modulus from the intrinsic resonant frequency of a clot [27]. Fibrin stiffness can be also measured using magnetic tweezers based on mechanical manipulation of magnetic beads embedded into the network [28-30].…”

Section: Viscoelastic Properties Of Fibrin (Fibrin Rheology)mentioning

confidence: 99%

Fibrin mechanical properties and their structural origins

2017

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Fibrin is a protein polymer that is essential for hemostasis and thrombosis, wound healing, and several other biological functions and pathological conditions that involve extracellular matrix. In addition to molecular and cellular interactions, fibrin mechanics has been recently shown to underlie clot behavior in the highly dynamic intra- and extravascular environment. Fibrin has both elastic and viscous properties. Perhaps the most remarkable rheological feature of the fibrin network is an extremely high elasticity and stability despite very low protein content. Another important mechanical property that is common to many filamentous protein polymers but not other polymers is stiffening occurring in response to shear, tension, or compression. New data has begun to provide a structural basis for the unique mechanical behavior of fibrin that originates from its complex multi-scale hierarchical structure. The mechanical behavior of the whole fibrin gel is governed largely by the properties of single fibers and their ensembles, including changes in fiber orientation, stretching, bending, and buckling. The properties of individual fibrin fibers are determined by the number and packing arrangements of double-stranded half-staggered protofibrils, which still remain poorly understood. It has also been proposed that forced unfolding of sub-molecular structures, including elongation of flexible and relatively unstructured portions of fibrin molecules, can contribute to fibrin deformations. In spite of a great increase in our knowledge of the structural mechanics of fibrin, much about the mechanisms of fibrin's biological functions remains unknown. Fibrin deformability is not only an essential part of the biomechanics of hemostasis and thrombosis, but also a rapidly developing field of bioengineering that uses fibrin as a versatile biomaterial with exceptional and tunable biochemical and mechanical properties.

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Section: Viscoelastic Properties Of Fibrin (Fibrin Rheology)mentioning

confidence: 99%

Fibrin mechanical properties and their structural origins

2017

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“…Their study showed that the system is sensitive to various fibrinogen contents and is capable to assess different CEM of purified clots formed with varying amounts of fibrinogen and thrombin. On the one hand, the RASOV has the potential to analyse the clot structure, composition and its functional mechanical properties, but on the other hand it is not able to measure coagulation dynamics like the oscillatory rheometer in this study [27].…”

Section: Discussionmentioning

confidence: 99%

“…One example of a novel clotting measurement was reported by Gongting et al [27]. The resonant acoustic spectroscopy with optical vibrometry (RASOV) measures the clot elastic modulus (CEM in kPa) via acoustic spectroscopy.…”

Section: Discussionmentioning

confidence: 99%

Dynamic and Quantitative Assessment of Blood Coagulation Status with an Oscillatory Rheometer

et al. 2018

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During open heart surgery, the haemostasis status of a patient has to be monitored permanently in order to quickly and reliably detect bleeding or coagulation-based disorders. Currently, no single medical device is available to provide a comprehensive solution for monitoring the coagulation status (coagulation, platelets and fibrinolysis). We intend to approach this problem with a rheological method. Here, we compared the performance of an oscillatory rheometer with a medical reference device, a ball coagulometer. Measuring the extrinsic coagulation (prothrombin time; PT), various heparin concentrations (0.5-2.0 IU/mL) could be differentiated and also discriminated from the intrinsic coagulation (activated partial thromboplastin time; aPTT) providing comparable clotting times between rheometer and ball coagulometer. In addition, the oscillatory rheometer was capable to detect the antagonising of heparin with the equimolar concentration of protamine and also the titration of various protamine concentrations (0.5-3.0 IU/mL) to a fixed heparin concentration (2 IU/mL). The addition of increasing concentrations of heparin to citrated blood prolonged the clotting time (CT), changed the slope calculated by linear regression of the elastic and viscous shear moduli (i.e., information of the coagulation process) and changed the value of the shear moduli at the end of the measurement (300 s). These results indicate that the oscillatory rheometer is capable to dynamically measure the haemostasis status with different activators and various inhibitor concentrations.

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“…While MM-OCT relies on the dynamic modulation of MNPs for image contrast, MM-OCE extracts the motion characteristics of the medium surrounding the MNPs in order to assess its biomechanical properties [1]. In MM-OCE, MNPs [1, 71-74] or magnetic microbeads [80, 81] can respond to a magnetic field and serve as force transducers. MNPs have negligible inertia compared to biological tissues, and hence the responsive motion reflects the viscoelasticity of the surrounding tissue in which the MNPs are embedded [49].…”

Section: Magnetomotive Nanoparticle Applicationsmentioning

confidence: 99%

Magnetomotive Optical Coherence Elastography for Magnetic Hyperthermia Dosimetry Based on Dynamic Tissue Biomechanics

Huang¹,

Pande

Ahmad

et al. 2016

IEEE J. Select. Topics Quantum Electron.

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Magnetic nanoparticles (MNPs) have been used in many diagnostic and therapeutic biomedical applications over the past few decades to enhance imaging contrast, steer drugs to targets, and treat tumors via hyperthermia. Optical coherence tomography (OCT) is an optical biomedical imaging modality that relies on the detection of backscattered light to generate high-resolution cross-sectional images of biological tissue. MNPs have been utilized as imaging contrast and perturbative mechanical agents in OCT in techniques called magnetomotive OCT (MM-OCT) and magnetomotive elastography (MM-OCE), respectively. MNPs have also been independently used for magnetic hyperthermia treatments, enabling therapeutic functions such as killing tumor cells. It is well known that the localized tissue heating during hyperthermia treatments result in a change in the biomechanical properties of the tissue. Therefore, we propose a novel dosimetric technique for hyperthermia treatment based on the viscoelasticity change detected by MM-OCE, further enabling the theranostic function of MNPs. In this paper, we first review the basic principles and applications of MM-OCT, MM-OCE, and magnetic hyperthermia, and present new preliminary results supporting the concept of MM-OCE-based hyperthermia dosimetry.

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High Sensitivity Micro-Elastometry: Applications in Blood Coagulopathy

Cited by 19 publications

References 26 publications

Fibrin mechanical properties and their structural origins

Fibrin mechanical properties and their structural origins

Dynamic and Quantitative Assessment of Blood Coagulation Status with an Oscillatory Rheometer

Magnetomotive Optical Coherence Elastography for Magnetic Hyperthermia Dosimetry Based on Dynamic Tissue Biomechanics

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