High-Sensitivity Method for Counting Low Concentrations of Particles in Liquids Using Centrifugation and Image Analysis

Yabusaki, Katsumi; Saitoh, Naoko; Hirono, Taisuke; Matsui, Hiroki; Okada, Hiraki

doi:10.1143/jjap.39.3641

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…Hirono group developed the image cytometry centrifugation (ICC) to count leukocytes (Yabusaki et al 1999(Yabusaki et al , 2000 and later extended their research to study theoretically and experimentally a microfluidic image cytometry by using micro-PIV flow visualization technique (Hirono et al 2008). In their research, numbers and sizes of particles flowing through a microchannel were measured simultaneously by image sequence analysis.…”

Section: Micro-piv Methodsmentioning

confidence: 99%

Methods for counting particles in microfluidic applications

Zhang

Chon

Pan

et al. 2009

Microfluid Nanofluid

118

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Microfluidic particle counters are important tools in biomedical diagnostic applications such as flow cytometry analysis. Major methods of counting particles in microfluidic devices are reviewed in this paper. The microfluidic resistive pulse sensor advances in sensitivity over the traditional Coulter counter by improving signal amplification and noise reduction techniques. Nanoporebased methods are used for single DNA molecule analysis and the capacitance counter is useful in liquids of low electrical conductivity and in sensing the changes of cell contents. Light-scattering and light-blocking counters are better for detecting larger particles or concentrated particles. Methods of using fluorescence detection have the capability for differentiating particles of similar sizes but different types that are labeled with different fluorescent dyes. The micro particle image velocimetry method has also been used for detecting and analyzing particles in a flow field. The general limitation of microfluidic particle counters is the low throughput which needs to be improved in the future. The integration of two or more existing microfluidic particle counting techniques is required for many practical on-chip applications.

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Section: Micro-piv Methodsmentioning

confidence: 99%

Methods for counting particles in microfluidic applications

Zhang

Chon

Pan

et al. 2009

Microfluid Nanofluid

118

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“…The standard suspensions described above consist of monodisperse polystyrene latex (PSL) particles suspended in water with the number concentration accurately determined. While the microscopic method has been the primary choice for determining the concentration of standard suspensions [8], attempts have also been made to develop a new method including a technique using centrifugal separation and image analysis [9]. In response to the need for a traceability chain in measurements of the number concentrations of liquid-borne particles, we have developed a national primary standard and have started to provide a calibration service for manufacturers of commercial standard suspensions.…”

Section: Introductionmentioning

confidence: 99%

Primary standard for the number concentration of liquid-borne particles in the 10 to 20 µm diameter range

Sakaguchi¹,

Ehara²

2010

Meas. Sci. Technol.

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The national primary standard for the number concentration of liquid-borne particles in the 10 to 20 µm diameter range has been developed at the National Institute of Advanced Industrial Science and Technology (AIST), Japan. The standard consists of a total number counting type flow cytometer (T-FCM) and an electronic balance. The T-FCM is a commercial flow cytometer modified so that the total number of particles in an aqueous suspension sampled in a test tube can be counted, and the electronic balance is used to determine the mass of the suspension. This standard is intended to be used for calibrating commercial standard suspensions of monodisperse polystyrene latex (PSL) particles. The measurand in the calibration is the mass-based number concentration (the particle number in a unit mass of a suspension), and the calibration capability covers the concentration range from 5 × 102 to 2 × 106 particles g−1. When the concentration of the suspension is higher than 2 × 103 particles g−1, the suspension is first diluted to about 1 × 103 particles g−1 to suppress the coincidence loss in particle counting by the T-FCM. The validity of the calibration with the T-FCM was examined by comparison with an independent method in which a scanning electron microscope (SEM) was used to determine the number concentration of particles deposited on a silicon wafer. For a suspension of 10 µm PSL particles with a concentration of approximately 1 × 106 particles g−1, the concentration values determined by the T-FCM and SEM methods were 1.042 × 106 and 1.035 × 106 particles g−1, respectively: The difference was less than 0.7%. The relative expanded uncertainty of the measurement by the T-FCM method with the coverage factor k = 2 was 4.4%.

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“…On the other hand, in other fields, laser light scattering imaging systems, which are based on practically the same principle as the imaging backscatter lidars, have already been applied to observing the behavior of particles suspended in clean rooms, and measuring particles generated in plasma etching chambers, nanoparticles in laser ablation plumes, and particles in liquids. [11][12][13][14] Those systems use lateral scattering of laser light (not backscattered light). Thus far, in such lidar techniques, few attempts have been made to measure directly the number and size of particles in an atmosphere with backscattered light.…”

mentioning

confidence: 99%

Remote Particle Counter Using Backscattered Light Imaging

Ohzu

2006

jjap

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An optical particle counter using a short laser pulse and a charge coupled devices (CCD) camera with a fast gate shutter is proposed for the remote and real-time monitoring of large particles suspended in air. The counter can be used to visualize the laser-induced backscattered light from the particles in a limited region around two hundreds of meters apart from the observation station. The number of the particles and a histogram of the backscattered light were derived through image data processing. The process used to determine the concentration and size distribution of the particles is described.

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High-Sensitivity Method for Counting Low Concentrations of Particles in Liquids Using Centrifugation and Image Analysis

Cited by 5 publications

References 13 publications

Methods for counting particles in microfluidic applications

Methods for counting particles in microfluidic applications

Primary standard for the number concentration of liquid-borne particles in the 10 to 20 µm diameter range

Remote Particle Counter Using Backscattered Light Imaging

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