High-sensitivity lens-coupled slow-scan CCD camera for transmission electron microscopy

Fan, G.Y.; Ellisman, Mark H.

doi:10.1016/0304-3991(93)90019-t

Cited by 43 publications

(15 citation statements)

References 12 publications

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“…Several papers on image converters coupled to CCD sensors used in electron microscopy have recently been published (Daberkow, Herrmann, Liu & Rau, 1991;Krivanek & Mooney, 1993;Fan & Ellisman, 1993;Ishizuka, 1993) showing results similar to ours. However, there are important differences in these approaches, since the image converters described do not include the XRII itself and use a YAG phosphor screen and a fibre-optical plate.…”

Section: Xrii/ccdsupporting

confidence: 75%

The point-spread function of X-ray image-intensifiers/CCD-camera and imaging-plate systems in crystallography: assessment and consequences for the dynamic range

Bourgeois¹,

Moy²,

Svensson³

et al. 1994

J Appl Cryst

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The point-spread functions (PSFs) of two X-ray CCD detectors and two imaging-plate (IP) scanners were compared using the monochromatic beam of the Materials Science beamline of the ESRF. The CCD detectors were a commercial medical type X-ray image intensifier (XRII) for energies above 20 keV and a tube with a beryllium window especially designed for the 5-50keV range, optically coupled to a high-resolution cooled charge-coupled-device (CCD) camera. The IP scanners were the Fuji BAS2000 and the Molecular Dynamics Phosphorlmager 400E, with plates from Fuji and Kodak. The PSFs were recorded using a 30 x 301am pinhole at energies from 8 to 41 keV under normal and highly saturated conditions. Special care was taken in the study of PSF wings. Dynamic-range capabilities in the presence of PSF bleeding were also measured using a standard X-ray generator. The Fuji and MD IP scanners give very similar PSFs when used with Fuji plates [circa 130 tam full width at half-maximum (FWHM), l l001.tm at 0.1% of peak maximum]. The Kodak plates showed a broader PSF than the Fuji plates, one which also increases with X-ray energy. The standard XRII/CCD gave the coarsest PSF (circa 4000 lam at 0.1% of peak maximum), whereas the finest PSF was recorded with the beryllium XRII/CCD used in magnified mode (9001am at 0.1%). The best dynamic range was obtained with the XRII/CCDs; the lowest with the Fuji scanner.

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Section: Xrii/ccdsupporting

confidence: 75%

The point-spread function of X-ray image-intensifiers/CCD-camera and imaging-plate systems in crystallography: assessment and consequences for the dynamic range

Bourgeois¹,

Moy²,

Svensson³

et al. 1994

J Appl Cryst

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“…For a long time, film was the only suitable choice for quality image recording in electron microscopy. This has changed recently with the development of the slow-scan CCD (SSC) camera , Krivanek et al, 1991, Daberkow et al, 1991, Kujawa and Krahl, 1992, Fan and Ellisman, 1993, and imaging plates (Sonoda et al, 1983, Mori et al, 1990, Ogura et al, 1994 for digital recording. Both the SSC camera and IP are highly linear and have a large dynamic range.…”

Section: Introductionmentioning

confidence: 99%

“…The lens coupling gives more flexibility in the camera design, but is less efficient than the fiber-optical coupling. For an example, the 45°a ngle lens coupling is employed to avoid the x-rays in high voltage electron microscopes (Fan and Ellisman, 1993). Imaging plates were initially developed in the 1980s for X-ray recording.…”

Section: Introductionmentioning

confidence: 99%

Electron detection characteristics of a slow-scan CCD camera, imaging plates and film, and electron image restoration

Zuo

2000

Microsc. Res. Tech.

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“…Unfortunately, in the CCD systems, each electron incident on the scintillator screen will produce a footprint, that limits the spatial resolution of the CCD detectors. The FWHM of beam broadening for a 300 KV electron beam is about 30 μm for a bright self supported scintillator screen (Fan & Ellisman, 1993; Jin, et al, 2008). Additionally, for fiber coupled scintillator based cameras, as a result of backscattering from the underlying fiber-optic plate, the effective beam broadening can be as high as 50–100 μm (Daberkow, et al, 1991; Fan & Ellisman, 2000).…”

Section: Resultsmentioning

confidence: 99%

Improving Signal to Noise in Labeled Biological Specimens Using Energy-Filtered TEM of Sections with a Drift Correction Strategy and a Direct Detection Device

et al. 2014

Self Cite

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Energy filtered transmission electron microscopy techniques are regularly used to build elemental maps of spatially distributed nanoparticles in materials and biological specimens. When working with thick biological sections, EELS techniques involving core-loss electrons often require exposures exceeding several minutes to provide sufficient signal to noise. Image quality with these long exposures is often compromised by specimen drift, which results in blurring and reduced resolution. To mitigate drift artifacts, a series of short exposure images can be acquired, aligned, and merged to form a single image. For samples where the target elements have extremely low signal yields, the use of CCD based detectors for this purpose can be problematic. At short acquisition times, the images produced by CCDs can be noisy and may contain fixed pattern artifacts that impact subsequent correlative alignment. Here we report on the use of direct electron detection devices (DDD’s) to increase the signal to noise as compared to CCD’s. A 3x improvement in signal is reported with a DDD vs. a comparably formatted CCD, with equivalent dose on each detector. With the fast rolling-readout design of the DDD, the duty cycle provides a major benefit, as there is no dead time between successive frames.

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High-sensitivity lens-coupled slow-scan CCD camera for transmission electron microscopy

Cited by 43 publications

References 12 publications

The point-spread function of X-ray image-intensifiers/CCD-camera and imaging-plate systems in crystallography: assessment and consequences for the dynamic range

The point-spread function of X-ray image-intensifiers/CCD-camera and imaging-plate systems in crystallography: assessment and consequences for the dynamic range

Electron detection characteristics of a slow-scan CCD camera, imaging plates and film, and electron image restoration

Improving Signal to Noise in Labeled Biological Specimens Using Energy-Filtered TEM of Sections with a Drift Correction Strategy and a Direct Detection Device

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