High-sensitivity imaging of time-domain near-infrared light transducer

Gu, Yuyang; Guo, Zhiyong; Yuan, Wei; Kong, Mengya; Liu, Yulai; Liu, Yongtao; Gao, Yixin; Feng, Wei; Wang, Fan; Zhou, Jiajia; Jin, Dayong; Li, Fuyou

doi:10.1038/s41566-019-0437-z

Cited by 174 publications

(128 citation statements)

References 37 publications

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“…Gu et al realized high-sensitivity TG imaging of NIR probes in a tumor-bearing mouse model, and the system was also based on OC-(iii) using an arbitrary waveform generator. 51 OC-(iv) is illustrated in Fig. 6, which has been reported very recently.…”

Section: Tg Implementation Based On Optical Choppersmentioning

confidence: 55%

“…Hybrid photodetectors, such as GaAsP hybrid photomultipliers and InGaAs emICCD, were also used for TG imaging. 25,51,61,62 Current-assisted photonic sampler arrays combined with CMOS sensors were developed for TG detection. 63 Time-correlated single photon counting (TCSPC) technique based on SPAD or PMT detectors was used in TG imaging.…”

Section: Detectionmentioning

confidence: 99%

“…In the last decade, TGLI of labeled mouse tissue has attracted much attention. 25,31,35,37,38,43,48,51,77,81,86 For a few examples, Joo et al presented the use of a Si NP probe with lifetime of microseconds for contrast improvement of > 100 fold by TGLI (versus CW imaging). 35 Figure 12 shows CW and TG images of mouse tissues (brain, liver, heart, kidney, lung, spleen, and tumor) before and after localized injection of porous Si NPs (PSiNPs) and molecular dyes (AF647).…”

Section: Tissuesmentioning

confidence: 99%

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Time-gated fluorescence imaging: Advances in technology and biological applications

Yang

Chen

2020

J. Innov. Opt. Health Sci.

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Time-gated (TG) fluorescence imaging (TGFI) has attracted increasing attention within the biological imaging community, especially during the past decade. With rapid development of light sources, image devices, and a variety of approaches for TG implementation, TGFI has demonstrated numerous biological applications ranging from molecules to tissues. The paper presents inclusive TG implementation mainly based on optical choppers and electronic units for synchronization of fluorescence excitation and emission, which also serves as guidelines for researchers to build suited TGFI systems for selected applications. Note that a special focus will be put on TG implementation based on optical choppers for TGFI of long-lived probes (lifetime range from microseconds to milliseconds). Biological applications by TG imaging of recently developed luminescent probes are described.

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Section: Tg Implementation Based On Optical Choppersmentioning

confidence: 55%

Section: Detectionmentioning

confidence: 99%

Section: Tissuesmentioning

confidence: 99%

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Time-gated fluorescence imaging: Advances in technology and biological applications

Yang

Chen

2020

J. Innov. Opt. Health Sci.

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“…Recently, we reported a family of rare‐earth nanocrystals with the absorption and emission at the same energy level, which has inspired our design. With time‐domain filtering technique, the NIR light transducers have strong luminescence and high quantum yield compared to upconversion materials, NIR dyes, or quantum dots …”

mentioning

confidence: 99%

“…A singly doped Tm 3+ nanocrystal with NaYF 4 matrix is produced with narrow size distribution ( Figure b). Time‐gated luminescence spectrum was recorded with a purpose‐built time‐gating spectrometer reported previously (Figure S1, Supporting Information) . The key concept here is to directly excite the Tm 3+ ( 3 H 6 ) from ground state and detect the luminescence coming from the same transition (Figure a).…”

mentioning

confidence: 99%

Luminescence Lifetime–Based In Vivo Detection with Responsive Rare Earth–Dye Nanocomposite

Kong

Liu

et al. 2019

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limited by either the few choices of fluorophores [8,9] or the difficulty of aqueous modification, [10] which is especially true when NIR absorption and emission are required. [11,12] Moreover, the above-mentioned fluorophores mostly interact with analytes in a quenching scheme which can be easily affected in complex biological media such as cytoplasm or body fluid, making it more challenging to produce reliable in vivo lifetime signals. [13] Alternatively, thanks to the well-shielded 4f-4f transition, the lifetime of rare-earthdoped nanocrystals is less likely to fluctuate in those conditions once the emitters are well protected, [14][15][16] and NIR-emissive Tm 3+ , Er 3+ , Yb 3+ , or Nd 3+ has been widely applied in in vivo imaging. [17][18][19][20][21] The longer lifetime of rare-earth-doped nanocrystals also benefits background-free bioimaging as the short-lived autofluorescence can be filtered by time gate. [22][23][24] However, the commonly used rareearth-doped nanoparticles usually have to be passivated by an inert shell to prevent the quenching of activators and emitters (Yb 3+ , Tm 3+ ), [25][26][27][28][29] while the fluorescence energy transfer is most sensitive of distance. [30] This causes a dilemma; when a thicker shell increases the fluorescence intensity, the energy transfer efficiency inevitably drops. Therefore, it is necessary to design a luminescent lifetime probe which can take both the strong luminescence signal and the appropriate energy transfer distance into account thus meeting the requirements of detection in vivo. Recently, we reported a family of rare-earth nanocrystals with the absorption and emission at the same energy level, which has inspired our design. With time-domain filtering technique, the NIR light transducers have strong luminescence and high quantum yield compared to upconversion materials, NIR dyes, or quantum dots. [31] Based on these findings, a highly luminescent (182 times compared to core upconversion particles and 33 times compared to core-shell upconversion particles under low power density, as shown in Figure 3) and lifetime-responsive LRET nanocomposite was built for effective in vivo sensing (Figure 1). NaYF 4 :Tm nanocrystal, which absorbs and emits photons in the same transition ( 3 H 6 -3 H 4 ), was employed as the donors (Figure 1b). It was further combined with a commercially available IR-820 dye as acceptors, which has a specific response to ClO − , forming an NIR ClO − -responsive LRET nanocomposite. The lifetime was affected by the number of window (660-950 and 1000-1500 nm). Herein, this work reports a lifetimeresponsive nanocomposite with both excitation and emission in the NIR I window (800 nm) and lifetime in the microsecond region. The incorporation of Tm 3+ -doped rare-earth nanocrystals and NIR dye builds an efficient energy transfer pathway that enables a tunable luminescence lifetime range. The NaYF 4 :Tm nanocrystal, which absorbs and emits photons at the same energy level, is found to be 33 times brighter than optimized core-shell upconversi...

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Morphology Modulation of Aggregation‐induced Emission

Yan

Guo

et al. 2022

Handbook of Aggregation‐Induced Emission

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High-sensitivity imaging of time-domain near-infrared light transducer

Cited by 174 publications

References 37 publications

Time-gated fluorescence imaging: Advances in technology and biological applications

Time-gated fluorescence imaging: Advances in technology and biological applications

Luminescence Lifetime–Based In Vivo Detection with Responsive Rare Earth–Dye Nanocomposite

Morphology Modulation of Aggregation‐induced Emission

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