High Sensitivity, High Selectivity SERS Detection of MnSOD Using Optical Nanoantennas Functionalized with Aptamers

Abstract

In this paper, we present the development of a highly sensitive, specific and reproducible nanobiosensor to detect one specific liver cancer biomarker, the manganese super oxide dismutase (MnSOD). The high sensitivity and reproducibility was reached by using SERS on gold nanostructures (nanocylinders and coupled nanorods) produced by electron-beam lithography (EBL). The specificity of the detection was provided by the use of a specific aptamer with high affinity to the targeted protein as a recognition element. With such a sensor, we have been able to observe the SERS signal of the MnSOD at concentrations down to the nM level and to show with negative control that this detection is specific due to the use of the aptamer. This latter issue has allowed us to detect the MnSOD in different body fluids (serum and saliva) at concentrations in the nM range. We have then demonstrated the effectiveness of our SERS nanobiosensor using aptamer as a bioreceptor for the detection of disease biomarker at low concentration and in complex fluids.

Localized surface plasmons in structures with linear Au nanoantennas on a SiO2/Si surface

“…The energy of the longitudinal mode, which is polarized along the antenna, depends on the structural parameters (the length, width, and height of the antennas, as well as the distance between them), the dielectric function of surrounding media and substrate materials, and can be varied within a wide spectral range from visible to far infrared or terahertz frequencies [11–14]. Nanoantennas exhibiting the LSPR in the optical spectral range are already used for surface-enhanced Raman scattering (SERS) [15–19], and for fluorescence enhancements [20–22]. Nanoantennas with the LSPR energy located in the infrared spectral region are considered as promising nanostructures for the detection of small amounts of both organic (down to attomoles) and inorganic substances, including semiconductor nanocrystals [18,23–27].…”

Emerging technologies for salivaomics in cancer detection

“…divided currently used technologies and devices according to the type of biomarker and cancer, as follows . The biomarker types are: Transcriptomic biomarkers: breast cancer (nanographene oxide‐polyethylene glycol methyl ether methacrylate with DNase 1 to detect microRNA‐10b and microRNA‐10a) , oral cancer (electrical controlled magnetic EC Sensor to detect microRNA‐200a) , prostate cancer [nano‐graphene oxide (nGO)/FAM‐anti‐miR‐21 to detect microRNA‐21 and nGO/Cy5‐anti‐miR‐141 to detect microRNA‐141] ; Genomic biomarkers: oral cancer [electrochemical sensor using endonuclease target recycling amplification to detect oral cancer overexpressed 1 (ORAOV1) or electric field‐induced release and measurement method for detection of epidermal growth factor receptor (EGFR) ]; Metabolomic biomarkers: oral cancer (wireless mouthguard ezymatic biosensor to detect uric acid or lactic acid ), gastric cancer (microfluidic optoelectronic or graphene based‐antimicrobial peptides with passive detection of Helicobacter pylori ); Proteomic biomarkers: liver cancer (surface enhanced Raman spectroscopy using optical nanoanntenas functionalized with aptamers for detection of MnSOD) , breast cancer [surface plasma resonance biosensor based on Au/ZnO thin films for carcinoma antigen 15‐3 (CA15‐3)] ; Multiplex: silicon nanowire field effect transistor for IL‐8 and TNF‐α . …”

Highly sensitive detection of estradiol by a SERS sensor based on TiO2 covered with gold nanoparticles

“…The surface needs a combination of a good enhancement over a large range of wavelengths -so that molecules with various fingerprints can be detected -while it should also be easy to fabricate at a reduced cost. In addition, the surface functionalization must guarantee the selection, detection and quantification of a target molecule, namely a biomarker [1][2][3], or a pollutant [4,5] dissolved in complex media such as blood, plasma, urine or river and sea water.…”