High sensitivity detection of human serum albumin using a novel magnetoelastic immunosensor

Liu, Rong; Guo, Xing; Wang, Jingzhe; Guo, Jianhua; Zhang, Yixia; Zhang, Wendong; Sang, Shengbo

doi:10.1007/s10853-019-03554-0

Cited by 15 publications

(13 citation statements)

References 34 publications

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“…In addition, the two characteristic absorption peaks around 225 nm and 280 nm were attributed to the C=O absorption of the HSA protein backbone and absorption of HSA amino acid residues, respectively. [ 18 ] The absorption peaks for HSA gradually decreased and moved towards longer wavelengths with continuous addition of hyperoside, this reconfirmed that the quenching mechanism was initiated by formation of a ground state complex. In addition, Figure 6(b) shows binding of HSA to hyperoside in the presence of V C .…”

Section: Resultsmentioning

confidence: 73%

Study on the interaction of hyperoside and human serum albumin in V_C and V_C‐free environments by spectroscopic and molecular docking techniques

Xin

Chen

et al. 2020

Luminescence

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The interaction between hyperoside and human serum albumin was studied in vitamin C (VC) and VC‐free environments using ultraviolet (UV)–vis absorption, fluorescence, circular dichroism spectra, and molecular docking techniques under simulated physiological conditions. The two environments had different influences on the secondary structure of human serum albumin (HSA). The α‐helix content was slightly increased from 50% to 51% in the VC environment and increased from 50% to 55% in the VC‐free environment. The thermodynamic parameters were ΔH° = −30.7 kJ⋅mol−1 and ΔS° = −23.4 mol−1⋅K−1 in the VC environment and ΔH° = −25.4 kJ⋅mol−1 and ΔS° = −11.4 J⋅mol−1⋅K−1 in the VC‐free environment. Through thermodynamics parameters, hydrophobic force played a dominant role in the whole environment. The binding constants were calculated to be 7.25 × 105 mol⋅L−1 and 9.76 × 105 mol⋅L−1 at 298 K and they declined with the rise in temperature. The two binding distances were 2.6 nm and 2.5 nm respectively at 298 K, indicating that fluorescence energy transfer occurred. The UV–vis spectra indicated that fluorescence quenching of the HSA–hyperoside complex was a static quenching process. Hyperoside could spontaneously bind to HSA at site I (subdomain IIA). Molecular docking elucidated the way to binding basically through hydrophobic and van der Waals force interactions. Moreover, molecular docking showed that the VC environment could influence binding of HSA and hyperoside by more H‐binding and less hydrophobic forces.

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Section: Resultsmentioning

confidence: 73%

Study on the interaction of hyperoside and human serum albumin in V_C and V_C‐free environments by spectroscopic and molecular docking techniques

Xin

Chen

et al. 2020

Luminescence

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“…The detection range of spectral correlation interferometry is narrow, while linearity is poor. Hence, a novel ME immunosensor was constructed by Liu et al [20] to detect HSA. Figure 3a shows antibody immobilization process of ME [15] Copyright 2016, AIP Publishing LLC.…”

Section: Specific Binding Between Antigen and Antibodymentioning

confidence: 99%

“…[6][7][8][9] Figure 1 shows an overview of the main approaches to using transducers in ME biosensors and application prospects in related fields. For instance, by using various kinds of surface functionalization and bioanalysis, ME biosensors are used to detect classical swine fever virus in wild animals, [10,11] monitor the release of pesticides, [12][13][14] and metal ions [15,16] into the environment, [17][18][19] and detect various diseases analyses (such as, diabetes, [20][21][22] genetic diseases [23][24][25] and cancers [26,27] ). Hence, the ME biosensor has a promising prospect in clinical use and environmental protection.…”

Section: Introductionmentioning

confidence: 99%

Surface Functionalization, Bioanalysis, and Applications: Progress of New Magnetoelastic Biosensors

Yuan

Huang

et al. 2022

Adv Eng Mater

Self Cite

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Magnetoelastic (ME) biosensors are portable, nontoxic, inexpensive, compatible with wireless networks, and have high sensitivity, accuracy, and specificity toward the target element. Thus, they have attracted considerable attention in application of biological identification, chemical detection, and medical diagnosis, as well as in many academic achievements in the field of sensor research. Herein, the recent progress of ME biosensors, including surface functionalization, bioanalysis, and its applications is critically reviewed. The remaining scientific and technological challenges related to the development of novel ME biosensors with new materials and structures in this field are elaborated, too. This review not only can provide a guideline for researchers in future development of ME biosensors, but also further improves their detection performance and expand the scope of their applications.

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“…Specifically, corrosion and degradation of ME materials can affect their performance as resonance frequencies are partially dependent on the material mass [18]. ME materials are often coated with layers of highly unreactive metals including gold or chromium via sputtering or thermal evaporation to aid in corrosion prevention [19,20]. Compared to chromium, gold is slightly more active on the metal reactivity series, but generally cheaper in cost.…”

Section: Me Substrate Materialsmentioning

confidence: 99%

“…Specifically, Bayhydrol 110 (Covestro AG: Leverkusen, Germany), an aliphatic polyester urethane resin, is a common polymer coating used with ME materials to enable bacterial cell adhesion [27,28]. Silane-based coatings can also be used to prevent corrosion of ME materials and provide biofunctionalized surfaces [19]. The thickness of polymer coatings on ME materials typically ranges from one to tens of micrometers.…”

Section: Me Substrate Materialsmentioning

confidence: 99%

Magnetoelastic Materials for Monitoring and Controlling Cells and Tissues

Meyers

Ong

2021

Sustainability

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Advances in cell and tissue therapies are slow to be implemented in the clinic due to the limited standardization of safety and quality control techniques. Current approaches for monitoring cell and tissue manufacturing processes are time and labor intensive, costly, and lack commercial scalability. One method to improving in vitro manufacturing processes includes utilizing the coupled magnetic and mechanical properties of magnetoelastic (ME) materials as passive and wireless sensors and actuators. Specifically, ME materials can be used in quantifying cell adhesion, detecting contamination, measuring biomarkers, providing biomechanical stimulus, and enabling cell detachment in bioreactors. This review outlines critical design considerations for ME systems and summarizes recent developments in utilizing ME materials for sensing and actuation in cell and tissue engineering.

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High sensitivity detection of human serum albumin using a novel magnetoelastic immunosensor

Cited by 15 publications

References 34 publications

Study on the interaction of hyperoside and human serum albumin in V_C and V_C‐free environments by spectroscopic and molecular docking techniques

Study on the interaction of hyperoside and human serum albumin in V_C and V_C‐free environments by spectroscopic and molecular docking techniques

Surface Functionalization, Bioanalysis, and Applications: Progress of New Magnetoelastic Biosensors

Magnetoelastic Materials for Monitoring and Controlling Cells and Tissues

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High sensitivity detection of human serum albumin using a novel magnetoelastic immunosensor

Cited by 15 publications

References 34 publications

Study on the interaction of hyperoside and human serum albumin in VC and VC‐free environments by spectroscopic and molecular docking techniques

Study on the interaction of hyperoside and human serum albumin in VC and VC‐free environments by spectroscopic and molecular docking techniques

Surface Functionalization, Bioanalysis, and Applications: Progress of New Magnetoelastic Biosensors

Magnetoelastic Materials for Monitoring and Controlling Cells and Tissues

Contact Info

Product

Resources

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Study on the interaction of hyperoside and human serum albumin in V_C and V_C‐free environments by spectroscopic and molecular docking techniques

Study on the interaction of hyperoside and human serum albumin in V_C and V_C‐free environments by spectroscopic and molecular docking techniques