High sensitivity and low-cost flavin luciferase (FLUXVc)-based reporter gene for mammalian cell expression

Phonbuppha, Jittima; Tinikul, Ruchanok; Ohmiya, Yoshihiro; Chaiyen, Pimchai

doi:10.1016/j.jbc.2023.104639

Cited by 2 publications

(1 citation statement)

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“…3C and 3D). Unlike previous reporter assays utilizing Lux (28), which relied on Fluc or Rluc to normalize luminescence intensity and required cell lysis in downstream processes, our NLX system eliminates the need for additional luciferases and the requirement for cell lysis. This feature enhances convenience and costeffectiveness, making it particularly advantageous for HTS applications.…”

Section: Application Of Nlxs As Gene Expressionmentioning

confidence: 99%

Autonomous multicolor bioluminescence imaging in bacteria, mammalian, and plant hosts

Kusuma,

Hattori,

Nagai

2024

Preprint

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Bioluminescence imaging has become a valuable tool in biological research, offering several advantages over fluorescence-based techniques, including the absence of phototoxicity and photobleaching, along with a higher signal-to-noise ratio. Common bioluminescence imaging methods often require the addition of an external chemical substrate (luciferin), which can result in a decrease in luminescence intensity over time and limit prolonged observations. Since the bacterial bioluminescence system is genetically encoded for luciferase-luciferin production, it enables autonomous bioluminescence (auto-bioluminescence) imaging. However, its application to multiple reporters is restricted due to a limited range of color variants. Here, we report five colors auto-bioluminescence system named Nano-lanternX (NLX), which can be expressed in bacterial, mammalian, and plant hosts, thereby enabling auto-bioluminescence in various living organisms. We have also expanded the applications of the NLX system, such as multiplexed auto-bioluminescence imaging for gene expression, protein localization, and dynamics of biomolecules within living mammalian cells.

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Section: Application Of Nlxs As Gene Expressionmentioning

confidence: 99%