High sensitive LC–MS/MS method for estimation of eluxadoline in human plasma and its application to pharmacokinetic study

Dodda, Sireesha; Ajitha, M.; Polagani, Srinivasa Rao; Kandhagatla, Raj Narayana

doi:10.1016/j.jpba.2018.11.056

Cited by 4 publications

(5 citation statements)

References 14 publications

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“…From the literature reports, no analytical approach for the simultaneous determination of eluxadoline and rifaximin in rat plasma has been described. Currently, there are few literature reports on the methods for the individual determination of eluxadoline have been reported [10][11][12][13] and for the individual estimation of rifaximin have been reported [14][15][16][17][18][19][20]. Even though these techniques have attained the requisite sensitivity and application, some of them require a considerable volume of plasma, many Whereas, the method used in the present study, HPLC-PDA has numerous advantages such as good separation, high sensitivity, outstanding specificity, and low cost as compared to the methods reported earlier.…”

Section: Discussionmentioning

confidence: 99%

“…In 2015, FDA approved Rifaximin (RFX) for the treatment of the gastrointestinal disorder (IBS-D) in adults. It is chemically designated as (2S,16Z,18E,20S,21S,22R,23-R,24R,25S,26R,27S,28E)-25-(Acetyloxy)-5,6,21,23-tetra hydroxy-27-methoxy-2,4,11,16,20,22,24,26-octamethyl-2,7-(epoxypentadeca [1,11,13]trienimino)benzofuro [4,5e]pyrido [1,2-a]benzimidazole-1,15(2H)-dione.…”

Section: Introductionmentioning

confidence: 99%

“…Eluxadoline has been determined in rat plasma using µ-SPE followed by LC-MS/MS [10]. The LC-MS/MS technique was used to investigate the pharmacokinetics of eluxadoline in healthy South Indian male participants [11]. RP-HPLC was used to separate and identify eluxadoline and its impurities [12].…”

Section: Introductionmentioning

confidence: 99%

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Validation of the HPLC–PDA method for detection of eluxadoline and rifaximin in rat plasma and application in a pharmacokinetic study

et al. 2022

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Background A precise, simple, accurate, and quick HPLC–PDA method for the determination of eluxadoline and rifaximin in rat plasma was developed and validated in this study. In this method, Loperamide hydrochloride was used as the internal standard and plasma samples were prepared using a liquid–liquid extraction technique for which acetonitrile was a solvent. An Agilent Symmetry C8 column (5 µm, 250 mm × 4.6 mm) at 283 nm and isocratic elution using HPLC grade acetonitrile and 7 mM TEA (pH 2.5) with a ratio of (40: 60 v/v) was used as a mobile phase and the flow rate employed was 1 mL min−1. A satisfactory chromatographic separation was accomplished. Results An HPLC–PDA method for the determination of eluxadoline and rifaximin with retention times of 3.06 and 7.82 min, respectively, was developed. The calibration curves appear linear for both eluxadoline and rifaximin in the range of 5–200 ng mL−1 and 10–400 ng mL−1, and the corresponding correlation coefficient values were found to be 0.9999 and 0.9998 respectively. Lower limits of quantification (LLOQ) for eluxadoline and rifaximin were evaluated to be 5.0 ng mL−1 and 10.0 ng mL−1, respectively. The accuracy and precision results in all validation experiments were within the acceptance limits of FDA guidelines. Conclusion The developed HPLC–PDA approach was fully validated to meet the USFDA guidelines for bioanalytical method validation in terms of precision, accuracy, and stability. The presented approach could be beneficial for the determination of ELX and RFX in rat plasma, according to validation parameters. This is one of the efficient method to study the pharmacokinetics of ELX and RFX in rats. Graphical abstract

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Validation of the HPLC–PDA method for detection of eluxadoline and rifaximin in rat plasma and application in a pharmacokinetic study

et al. 2022

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“…The validation of the developed method was performed as per USFDA guidelines [22] and our previous work [23] .…”

Section: Methodsmentioning

confidence: 99%

Bioanalysis of bedaquiline in human plasma by liquid chromatography-tandem mass spectrometry: Application to pharmacokinetic study

Bezawada,

Mogili,

Rao Polagani

et al. 2024

Journal of Mass Spectrometry and Advances in the Clinical Lab

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“…Liquid chromatography-mass spectrometry (LC-MS) is another important platform for the analysis of unstable, nonvolatile, non-polar compounds without derivatization of samples (Dodda et al, 2018;Zhao and Li, 2018). Hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS) is a high throughput intracellular metabolomics analysis technology that can analyze polar and non-polar metabolites simultaneously (Teleki and Takors, 2019).…”

Section: Mass Spectrometrymentioning

confidence: 99%

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

Meng¹,

LI²,

YANG³

et al. 2023

Biocell

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In recent years, microbial metabolomics, a new field that has attracted wide attention, provides a map of metabolic pathways and clarifies the interaction mechanism between microorganisms and hosts. Many microorganisms are found in the human intestine, oral cavity, vagina, etc. Probiotics could maintain the good health of the host, while pathogens and an imbalance of bacterial flora lead to a series of diseases of the body and mind.Metabolomics is a science for qualitative and quantitative analysis of all metabolites in an organism or biological system, which could provide key information to understand the related metabolic pathways and associated changes. This approach analyzes the final products of cellular regulatory processes, the level of which can be regarded as the ultimate response of the biological system to genetic or environmental changes. Microbial metabolomics has been widely used in different research fields, such as microbial phenotypic classification, mutant screening, metabolic pathways, microbial metabolic engineering, fermentation engineering monitoring and optimization, microbial environmental pollution, and so on. However, there are only a few reviews on microbial metabolomics of probiotics and pathogens. This review summarizes the main methodologies, including sample preparation, identification of metabolites, data processing, and analysis. Recent applications in microbial metabolomics of probiotics and pathogens are also described. This paper first summarized the research progress and application of microbial metabolomics from two aspects: probiotics and pathogenic bacteria. Probiotics and pathogenic bacteria do not exist independently most of the time; hence, these were reviewed in the research field of coexistence of probiotics and pathogenic bacteria, which was subdivided into important microbial research fields closely related to human health, including the human gut, oral cavity, food, and nutrition-related microorganisms. Then, the main problems and trends associated with microbial metabolomics are discussed.

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High sensitive LC–MS/MS method for estimation of eluxadoline in human plasma and its application to pharmacokinetic study

Cited by 4 publications

References 14 publications

Validation of the HPLC–PDA method for detection of eluxadoline and rifaximin in rat plasma and application in a pharmacokinetic study

Validation of the HPLC–PDA method for detection of eluxadoline and rifaximin in rat plasma and application in a pharmacokinetic study

Bioanalysis of bedaquiline in human plasma by liquid chromatography-tandem mass spectrometry: Application to pharmacokinetic study

Review on microbial metabolomics of probiotics and pathogens: Methodologies and applications

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