High-sensitive electrochemical detection of point mutation based on polymerization-induced enzymatic amplification

Feng, Keqin; Zhao, Jingjin; Wu, Zai‐Sheng; Jiang, Jian‐Hui; Shen, Guo‐Li; Yu, Ru‐Qin

doi:10.1016/j.bios.2010.12.024

Cited by 19 publications

(5 citation statements)

References 46 publications

(39 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[4] Such platforms must also support multiplexed detection and accurately differentiate between homozygous and heterozygous mismatches -a critical factor for many diagnoses. [5,6] Electrochemical DNA sensors offer robustness, portability, and compatibility with microfluidics and microelectronics, [7,8] and many varieties of electrochemical DNA sensors (as reviewed by Palecek and Bartosik) [9] have been developed for SNP detection, including various enzymatic [10][11][12] and hybridization-based assays. [13][14][15] Surface-immobilized, redoxreporter labeled probes offer many advantages in this arena as they require no exogenous reagents, exhibit low background, and can operate directly in complex mixtures.…”

Section: Introductionmentioning

confidence: 99%

Accurate Zygote‐Specific Discrimination of Single‐Nucleotide Polymorphisms Using Microfluidic Electrochemical DNA Melting Curves

et al. 2014

View full text Add to dashboard Cite

We report the first electrochemical SNP detection system that can accurately discriminate homozygous and heterozygous genotypes using microfluidics technology. To achieve this, our system performs real-time melt-curve analysis of surface immobilized hybridization probe. As an example, we used our sensor to genotype two SNPs in the apolipoprotein E (ApoE) gene, where homozygous and heterozygous mutations greatly affect the risk of late-onset Alzheimer’s disease. Using probes specific for each SNP, we simultaneously acquired melt curves for probe-target duplexes at two different loci and thereby accurately distinguish all six possible ApoE allele combinations. Since the design of our device and probes can be readily adapted for targeting other loci, we believe that our method offers a modular platform for SNP-based disease diagnosis and personalized medicine.

show abstract

Section: Introductionmentioning

confidence: 99%

Accurate Zygote‐Specific Discrimination of Single‐Nucleotide Polymorphisms Using Microfluidic Electrochemical DNA Melting Curves

et al. 2014

View full text Add to dashboard Cite

show abstract

“…The LOD for the detection of SNPs by this assay was found to be in the order of 10 –16 M, which is a very impressive value reported for the detection of SNPs. Up to now, a number of amplication-free detection approaches have been developed for the detection of SNPs for β-thalassemia by various biorecognition strategies, including the oligonucleotides hybridization reaction, , the primer extension reaction, the enzymatic cleavage reaction, and the ligation reaction . Compared to these methods, our biosensor approach is simple and has better SNP discrimination than the oligonucleotides hybridization approach, exhibits superior sensitivity at fM levels, and offers a wide linear dynamic range of 7 orders of magnitude in concentration for SNP detection.…”

Section: Discussionmentioning

confidence: 99%

Noninvasive Prenatal Genetic Screening of Cell-Free Fetal DNA for Early Prediction of β-Thalassemia Using Fiber Optic Nanogold-Linked Sorbent Assay

Ngo,

Chaudhari,

Wang

et al. 2024

ACS Sens.

View full text Add to dashboard Cite

β-Thalassemia is a prevalent type of severe inherited chronic anemia, primarily identified in developing countries. The identification of single nucleotide polymorphisms (SNPs) plays a vital role in the early diagnosis of genetic diseases. Here, we reported the development of an amplification-free fiber optic nanogold-linked sorbent assay method using a fiber optic particle plasmon resonance (FOPPR) biosensor for rapid and ultrasensitive detection of SNPs. Herein, MutS protein was selected as the biorecognition capture probe and immobilized on the sensing region to capture the target mutant DNA, which was hybridized with a single-base mismatched single-stranded DNA labeled by a gold nanoparticle (AuNP). The AuNP acts as a signaling agent to be detected by the FOPPR biosensor when it is bound on the fiber core surface. The method effectively differentiates mismatched double-stranded DNA by MutS protein from perfectly matched/ complementary dsDNA. It exhibits an impressively low detection limit for the detection of SNPs at approximately 10 −16 M using low-cost sensor chips and devices. By determination of the ratio of mutant DNA to normal DNA in cell-free genomic DNA from blood samples, this method is promising for diagnosing β-thalassemia in fetuses without invasive testing techniques.

show abstract

“…Since physiological factors and mutated genes aflC or aflR affect aflatoxin biosynthesis in A. flavus, [31] the aflD gene will not detect gene expression even though this system can be developed by using quantum-dot (QD) electrochemical, [32] restriction endonuclease-based electrochemical biosensor technology, [33] and a polymerization-induced enzymatic amplification-based electrochemical biosensor [34] for the screening of point mutation in the gene. Therefore, this technique is more useful for enumeration of A. flavus rather than the toxicity detection.…”

Section: Discussionmentioning

confidence: 99%

Detection ofaflDgene in contaminated pistachio withAspergillus flavusby DNA based electrochemical biosensor

Sedighi-Khavidak

Mazloum-Ardakani

Khorasgani

et al. 2017

International Journal of Food Properties

View full text Add to dashboard Cite

Aspergillus flavus detection and the removal of contaminated pistachio are critically important. In this work, an impedimetric electrochemical DNA sensor using gold nanoparticles on the glassy carbon electrode was developed for the sensitive detection of the aflD gene of A. flavus. This biosensor had a linear range of the detection, which was from 1 nM to 10 µM with the detection limit of 0.55 nM, while the best time for hybridization was 4 hr. Results for the detection and reproducibility of the synthetic 21-nucleotide target and the target 76-nucleotide polymerase chain reaction product were the same due to the sensitivity of measurement. In addition, this biosensor can be used to enumerate A. flavus up to 2.5 × 10 8 spores/mL.

show abstract

High-sensitive electrochemical detection of point mutation based on polymerization-induced enzymatic amplification

Cited by 19 publications

References 46 publications

Accurate Zygote‐Specific Discrimination of Single‐Nucleotide Polymorphisms Using Microfluidic Electrochemical DNA Melting Curves

Accurate Zygote‐Specific Discrimination of Single‐Nucleotide Polymorphisms Using Microfluidic Electrochemical DNA Melting Curves

Noninvasive Prenatal Genetic Screening of Cell-Free Fetal DNA for Early Prediction of β-Thalassemia Using Fiber Optic Nanogold-Linked Sorbent Assay

Detection ofaflDgene in contaminated pistachio withAspergillus flavusby DNA based electrochemical biosensor

Contact Info

Product

Resources

About