High-resolution two-photon fluorescence microscope imaging of nanodiamonds containing NV color centers

Versatile nanodiamond-based tools for therapeutics and bioimaging

“…Successful imaging of fluorescent NDs by two-photon excitation with infrared pico-or femtosecond pulses has been demonstrated. 193,194 Further investigations of this two-photon excitation imaging technique of NDs in cells or in animals are required for potential applications in biology, physics and materials science.…”

Light-Activated Nanodiamond-Based Drug Delivery Systems for Spatiotemporal Release of Antisense Oligonucleotides

“…2−4 Notably, NDs possess negatively charged nitrogen-vacancy (NV − ) centers, which serve as nonphotobleaching fluorophores for long-term particle tracking and target sensing using fluorescence or magneto-optical detection techniques. 5,6 We have successfully modified the surface of ND with various functional ligands, including polymers, proteins, and peptides, enabling targeted drug delivery to specific organelles and enhancing gene therapy. 7,8 Recently, our group and Weil's group demonstrated that protein-coated NDs, exhibiting high dispersity, can traverse the blood-brain barrier (BBB) in mice following tail vein injection.…”

“…However, some of these agents may exhibit undesirable side effects, and their biological functions can be significantly compromised because of off-target reactions and inadequate control over their release in tissue following systematic administration. Nanodiamonds (NDs) have emerged as a promising carbon-based material for drug delivery due to their unique features, such as versatile functionalization, a high surface areas-to-volume ratio, biocompatibility, and strong affinity to biomolecules. − Notably, NDs possess negatively charged nitrogen-vacancy (NV − ) centers, which serve as nonphotobleaching fluorophores for long-term particle tracking and target sensing using fluorescence or magneto-optical detection techniques. , We have successfully modified the surface of ND with various functional ligands, including polymers, proteins, and peptides, enabling targeted drug delivery to specific organelles and enhancing gene therapy. , Recently, our group and Weil’s group demonstrated that protein-coated NDs, exhibiting high dispersity, can traverse the blood-brain barrier (BBB) in mice following tail vein injection. , The inherent photophysical properties and excellent biocompatibility of NDs position them as versatile tools for both diagnostic and therapeutic applications …”

Determining the Dependence of Single Nitrogen−Vacancy Center Light Extraction in Diamond Nanostructures on Emitter Positions with Finite−Difference Time−Domain Simulations

“…The nitrogen−vacancy (NV) center of diamonds has been identified as an excellent candidate for a wide range of applications, such as quantum computation and communication [ 7 , 8 ], biological detection [ 9 , 10 , 11 , 12 ], and high−precision quantum sensors for electromagnetic, temperature, stress, and pressure fields [ 13 , 14 , 15 , 16 , 17 ]. The NV color center has a stable photon emission performance at room temperature, and its electron spin has a long coherence time (T 2 = 1.8 ms) [ 18 ], which is sensitive to changes in the surrounding environment and causes corresponding feedback that can be optically detected in the form of the spin state of the color center.…”