High‐resolution three‐dimensional visualization of the rat spinal cord microvasculature by synchrotron radiation micro‐CT

Hu, Jianzhong; Cao, Yong; Wu, Tianding; Li, Danzhen; Lü, Hongbin

doi:10.1118/1.4894704

Cited by 37 publications

(29 citation statements)

References 36 publications

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“…These high precision and high fidelity 3D structural parameters further enable our study of the minute modification of renal microvascularture 52–55 . As a benefit of the superior performance, for example, that the number of microvessels determined by our imaging method was significantly higher than that of lab-based micro CTs 56 .…”

Section: Discussionmentioning

confidence: 98%

“…S1, for example showing an X-ray image of a nanofabricated Au test pattern) with very fast imaging acquisition and processing. Our X-ray imaging platform also features nanoscale resolution and has been successfully applied to investigate tumor angiogenesis at tissue and cell level 20–26 . For this work specifically, the microtomography is suitable for exploring the kidney microvasculature 27–29 and nanotomography supplemented the detailed examination.…”

Section: Introductionmentioning

confidence: 99%

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Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy

Lin

Hwu

Huang

et al. 2017

Sci Rep

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High resolution synchrotron microtomography capable of revealing microvessels in three dimensional (3D) establishes distinct imaging markers of mouse kidney disease strongly associated to renal tubulointerstitial (TI) lesions and glomerulopathy. Two complementary mouse models of chronic kidney disease (CKD), unilateral ureteral obstruction (UUO) and focal segmental glomerulosclerosis (FSGS), were used and five candidates of unique 3D imaging markers were identified. Our characterization to differentially reflect the altered microvasculature of renal TI lesions and/or glomerulopathy demonstrated these image features can be used to differentiate the disease status and the possible cause therefore qualified as image markers. These 3D imaging markers were further correlated with the histopathology and renal microvessel-based molecular study using antibodies against vascular endothelial cells (CD31), the connective tissue growth factor or the vascular endothelial growth factor. We also found that these 3D imaging markers individually characterize the development of renal TI lesions or glomerulopathy, quantitative and integrated use of all of them provide more information for differentiating the two renal conditions. Our findings thus establish a practical strategy to characterize the CKD-associated renal injuries by the microangiography-based 3D imaging and highlight the impact of dysfunctional microvasculature as a whole on the pathogenesis of the renal lesions.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy

Lin

Hwu

Huang

et al. 2017

Sci Rep

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“…35 The spatial resolution of SRA can reach submicrons, approximately 1000 times higher than that of conventional X-ray absorption imaging, and it can be used for high-resolution imaging of the whole middle cerebral artery territory blood supply in living rodents. 53,54 Vessel density is an indicator of microvascular perfusion. Our results showed that NBP treatment increased the perfused vessel density.…”

Section: Discussionmentioning

confidence: 99%

Dl‐3‐N‐butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats

Zhou

Wang

et al. 2019

CNS Neurosci Ther

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Summary Introduction Dl‐3‐N‐butylphthalide (NBP), a small molecule drug used clinically in the acute phase of ischemic stroke, has been shown to improve functional recovery and promote angiogenesis and collateral vessel circulation after experimental cerebral ischemia. However, the underlying molecular mechanism is unknown. Aims To explore the potential molecular mechanism of angiogenesis induced by NBP after cerebral ischemia. Results NBP treatment attenuated body weight loss, reduced brain infarct volume, and improved neurobehavioral outcomes during focal ischemia compared to the control rats ( P < 0.05). NBP increased the number of CD31 + microvessels, the number of CD31 + /BrdU + proliferating endothelial cells, and the functional vascular density ( P < 0.05). Further study demonstrated that NBP also promoted the expression of vascular endothelial growth factor and angiopoietin‐1 ( P < 0.05), which was accompanied by upregulated sonic hedgehog expression in astrocytes in vivo and in vitro. Conclusion NBP treatment promoted the expression of vascular endothelial growth factor and angiopoietin‐1, induced angiogenesis, and improved neurobehavioral recovery. These effects were associated with increased sonic hedgehog expression after NBP treatment. Our results broadened the clinical application of NBP to include the later phase of ischemia.

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“…All the relevant results are compatible, so the theoretically derived relative accuracy defined by equation (4) was verified and seems to be valid. Figure 8 shows theoretical accuracy ("Declared accuracy") and true accuracy 7 with errorbars presenting variance of measurements ("True accuracy"). Entire set of measured values stayed below the declared accuracy, what shows, that this accuracy estimation is very pessimistic, and real accuracy seems to be better ("Apparent real accuracy").…”

Section: Resultsmentioning

confidence: 99%

“…Finally, computer 3D models are, due to its plasticity, becoming more and more used for visualization of objects, which are unreachable (contaminated, dangerous or remote areas) [5], [6] or environments, which are invisible without invasive surgery (human inner structures) [7]. Another objects are visible, but its important details are too tiny (human outer structures) and it is necessary to enlarge them plastically [8].…”

Section: Introductionmentioning

confidence: 99%

Application of High-Resolution 3D Scanning in Medical Volumetry

Chromý¹

2016

International Journal of Electronics and Telecommunications

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Abstract-This paper deals with application of 3D scanning technology in medicine. Important properties of 3D scanners are discussed with emphasize on medical applications. Construction of medical 3D scanner according to these specifications is described and practical application of its use in medical volumetry is presented. Besides volumetry, such 3D scanner is usable for many other purposes, like monitoring of recovery process, ergonomic splint manufacturing or inflammation detection.3D scanning introduces novel volumetric method, which is compared with standard methods. The new method is more accurate compared to present ones. Principles of this method are discussed in paper and its accuracy is evaluated and experimentally verified.

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High‐resolution three‐dimensional visualization of the rat spinal cord microvasculature by synchrotron radiation micro‐CT

Cited by 37 publications

References 36 publications

Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy

Differential synchrotron X-ray imaging markers based on the renal microvasculature for tubulointerstitial lesions and glomerulopathy

Dl‐3‐N‐butylphthalide promotes angiogenesis and upregulates sonic hedgehog expression after cerebral ischemia in rats

Application of High-Resolution 3D Scanning in Medical Volumetry

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