High‐resolution ¹H NMR and magic angle spinning NMR spectroscopic investigation of the biochemical effects of 2‐bromoethanamine in intact renal and hepatic tissue

Abstract

The metabolic consequences of xenobiotic-induced toxicity were investigated using high-resolution magic angle spinning (MAS) NMR spectroscopy of intact tissue. Renal papillary necrosis (RPN) was induced in Sprague-Dawley rats (n ‫؍‬ 12) via a single i.p. dose of 250 mg/kg 2-bromoethanamine (BEA) hydrobromide. At 2, 4, 6, and 24 h after treatment with BEA, three animals were killed and tissue samples were obtained from liver, renal cortex, and renal medulla. Tissue samples were also removed at 2 and 24 h from matched controls (n ‫؍‬ 6). 1

In vivo biodistribution and biological impact of injected carbon nanotubes using magnetic resonance techniques

“…[28][29][30] It was performed in this study to assess the biological impact of SWCNT injection on liver metabolism as a general index of toxicity. HR-MAS 1 H NMR spectroscopy of intact tissue enables the simultaneous measurement of both lipids and low molecular weight metabolites, which is important in a toxicological diagnosis.…”

Organ-specific response to inhibition of mitochondrial metabolism by cyclosporine in the rat

“…The decrease in PUFA can be attributed to the increased stimulation of fatty acid oxidation which is another additional alternative pathway for energy production [ 101 when the oxidative mitochondrial pathway is inhibited by CyA. However, this adaptive process that results in lipid peroxidation is known to be harmful to the renal cortex and medulla [6]. Indeed, various metabolic markers for nephrotoxicity were found in rat kidney tissue treated with CyA.…”

Metabolomics: Concept, methods and potential prospect in marine biology

“…Magic Angle Spinning NMR (MAS-NMR) involves the detection of intact tissues and cells with little or no preparation [53,54]. MAS-NMR significantly reduces the effect of magnetic field inhomogeneity in ordered materials.…”