High Resolution Structure of the Manganese Derivative of Insulin

Prugovečki, Biserka; Pulić, Ivana; Toth, Martina; Matković-Čalogović, Dubravka

doi:10.5562/cca2108

Cited by 8 publications

(6 citation statements)

References 20 publications

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“…was explained by the higher radiation doses in the diffraction experiment. The observed nickel coordination is generally in very good agreement with the human Ni insulin structure (PDB entry 3exx; Prugovečki et al, 2009).…”

Section: Figuresupporting

confidence: 67%

“…below 0.3 Å . In analogy to bovine zinc T 6 insulin, comparison with the structures of human Ni insulin and Cu insulin [PDB entries 3exx (Prugovečki et al, 2009) and 3tt8 (Prugovečki & Matković-Č alogović, 2011)] show larger discrepancies (r.m.s.d.s around 1.3 Å ) owing to a different conformation of the B1.2-B3.2 chain. The weak determination of the B1.2-B3.2 residues may be a consequence of a partially disordered N-terminus of the B chain.…”

Section: General Conformationmentioning

confidence: 99%

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Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T₆bovine insulin derivatives

Frankær

Mossin

Ståhl

et al. 2013

Acta Cryst D Biol Crystallogr

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The level of structural detail around the metal sites in Ni 2+ and Cu 2+ T 6 insulin derivatives was significantly improved by using a combination of single-crystal X-ray crystallography and X-ray absorption spectroscopy. Photoreduction and subsequent radiation damage of the Cu 2+ sites in Cu insulin was followed by XANES spectroscopy.

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Section: Figuresupporting

confidence: 67%

Section: General Conformationmentioning

confidence: 99%

Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T₆bovine insulin derivatives

Frankær

Mossin

Ståhl

et al. 2013

Acta Cryst D Biol Crystallogr

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“…3). This coordination sphere is the same as in the native 2Zn-insulin and other metal derivatives such as manganese [18] and nickel [15] that were crystallized in low chloride concentration (less than 6%). The structure of the porcine 2Co-insulin revealed the same coordination sphere of the cobalt ion with the Co-N distances of 2.08 and 2.16 Å.…”

Section: Coordination Of the Cobalt Ionmentioning

confidence: 95%

“…It has been known for many years that different metal ions can substitute the zinc ions in the 2Zn hexamer [12], however only a few such complexes have been structurally characterized [13][14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Crystal structure of the cobalt human insulin derivative

Prugovečki¹,

Ivetić²,

Matković‐Čalogović³

2015

Maced. J. Chem. Chem. Eng.

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The structure of the human cobalt insulin derivative at 1.73 Å resolution is described. Single crystals were prepared by the hanging drop vapour diffusion crystallization method using Zn-free insulin and cobalt(II) acetate.The crystal structure was determined by the single crystal X-ray diffraction method. The investigated insulin derivative exhibits the T 6 form of insulin and crystallizes in the trigonal system in space group R3, with the unit cell parameters a = b = 81.43 Å and c = 33.75 Å. There are two cobalt atoms per insulin hexamer which are octahedrally coordinated by three symmetry-related N ε2 atoms of three HisB10/HisD10 and three oxygen atoms from three water molecules.Keywords: insulin derivative; cobalt; X-ray structure КРИСТАЛНА СТРУКТУРА НА ХУМАН КОБАЛТЕН ИНСУЛИНСКИ ДЕРИВАТРешена е кристалната структура на хуман кобалтен инсулински дериват со резолуција од 1,73 Å. Монокристалите се добиени со помош на методот на парна дифузија по принципот на висечка капка, користејќи инсулин (без Zn) и кобалт(II)ацетат. Кристалната структура е решена со рендгенска дифракција на монокристал. Изучуваниот дериват на инсулинот претставува форма Т 6 на инсулин и кристализира во тригоналниот систем, просторна група R3, со параметри на елементарната ќелија, a = b = 81,43 Å и c = 33,75 Å. Во инсулинскиот хексамер постојат два кобалтни атома кои се октаедарски координирани со три симетриски поврзани атоми N ε2 од три HisB10/HisD10 и три кислородни атоми од три молекули на вода.Клучни зборови: инсулински дериват; кобалт; рендгенска структура

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“…[4][5][6] Insulin is a small protein with a molecular weight of approximately 5800 g/mol and a molecular radius of 1.34 nm. [7][8][9] This protein plays a major role in the control of hyperglycaemia for patients with type 1 diabetes and selected patients with type 2 diabetes, by regulating blood-glucose levels.…”

mentioning

confidence: 99%

Visual in Solution Detection of Denatured Insulin Coupled to Gold Nanoparticles in the Presence of an Aminosilane

Farrell

Hawley

Reaume

et al. 2016

J. Electrochem. Soc.

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The pharmaceutical industry is comprised of a myriad of active pharmaceutical ingredients, all applied to increasing human health and quality of life. One of the requirements of reaping the health benefits of these drug products is maintaining them in a native or desired form. Such non-native forms can include, aggregated or fragmented structures. Unfortunately, the most widely used methods to detect non-native or denatured proteins require trained technicians, bulky instrumentation and large amounts of reagents. Deviation from the native structures can occur at all stages; from manufacturing and processing to storage. With these limitations in mind, a simplistic and highly sensitive in solution detection method was evaluated to visually detect denatured insulin proteins, utilizing gold nanoparticle aggregation via 3-Aminopropyltreithoxysilane. The insulin in this study was heat stressed using an 80 • C water bath to create an accelerated heat stressed environment. The insulin, gold nanoparticle and aminosilane solution was then characterized utilizing, UV-Vis spectroscopy, dynamic light scattering and scanning electron microscopy. Captured images and resulting absorbance spectra of the trials demonstrated visual color changes detectable with the human eye as a function of the denaturation time. This work serves as an extended proof of concept for fast in solution detection methods for proteins that have experienced heat stress.

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High Resolution Structure of the Manganese Derivative of Insulin

Cited by 8 publications

References 20 publications

Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T₆bovine insulin derivatives

Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T₆bovine insulin derivatives

Crystal structure of the cobalt human insulin derivative

Visual in Solution Detection of Denatured Insulin Coupled to Gold Nanoparticles in the Presence of an Aminosilane

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High Resolution Structure of the Manganese Derivative of Insulin

Cited by 8 publications

References 20 publications

Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T6bovine insulin derivatives

Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T6bovine insulin derivatives

Crystal structure of the cobalt human insulin derivative

Visual in Solution Detection of Denatured Insulin Coupled to Gold Nanoparticles in the Presence of an Aminosilane

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Product

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Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T₆bovine insulin derivatives

Towards accurate structural characterization of metal centres in protein crystals: the structures of Ni and Cu T₆bovine insulin derivatives