High-resolution phylogenetic analysis of NO2--oxidizing Nitrobacter species using the rrs-rrl IGS sequence and rrl genes.

Abstract

A high-resolution phylogenetic analysis of Nitrobacter strains and their neighbours was made using the rrs-rrl intergenic spacer sequence and the hypervariable part of the rrl gene. The phylogenetic tree obtained was consistent with that which was obtained previously but was much more discriminating, permitting the design of genus-specific primers.

Bosea eneae sp. nov., Bosea massiliensis sp. nov. and Bosea vestrisii sp. nov., isolated from hospital water supplies, and emendation of the genus Bosea (Das et al. 1996)

“…This approach has already been used for other members of the a-Proteobacteria, including Nitrobacter spp. (Grundmann et al, 2000) and Bradyrhizobium spp. (van Berkum & Fuhrmann, 2000).…”

Description of Afipia birgiae sp. nov. and Afipia massiliensis sp. nov. and recognition of Afipia felis genospecies A.

“…Nevertheless, the position of these bacteria within the Bradyrhizobium group is unclear. Within the Bradyrhizobium group, two major clusters can be distinguished : one that includes Bradyrhizobium elkanii (Kuykendall et al, 1992) and Afipia genospecies 1 and 2 (Brenner et al, 1991) and the other including Bradyrhizobium japonicum and Bradyrhizobium liaoningense (Jordan, 1982 ;Xu et al, 1995), all other Afipia species and members of the genera Nitrobacter (Grundmann et al, 2000 ;Navarro et al, 1992 ;Orso et al, 1994 ;Sorokin et al, 1998), Agromonas (Ohta & Hattori, 1983), Blastobacter (Hirsch & Mu$ ller, 1985) and Rhodopseudomonas (Hougardy et al, 2000). Moreover, several 16S rRNA gene sequences of Afipia genospecies available in GenBank (Afipia genospecies 4-14), for which no description or strain is available, are distributed among very distant groups.…”

“…In this study, A. felis and A. felis genospecies A represent two distinct genospecies on the basis of DNA-DNA hybridization and phenotypic data such as susceptibility to antibiotics, SDS-PAGE profile and whole-cell fatty acid composition (Wayne et al, 1987 ;Grimont et al, 1998), whereas they exhibit levels of 16S rDNA sequence similarity of 99n9%. An approach comparing sequences from more divergent parts of the genome such as the rrs-rrl intergenic spacer has been described for Nitrobacter species (Grundmann et al, 2000) and Bradyrhizobium species (van Berkum et al, 2000) and seems to be more powerful for differentiation. It therefore appears that even slight divergence in 16S rDNA sequences of bacteria of this group should be considered as significant.…”

“…2), such as a cluster with Nitrobacter species, which is in accordance with the denomination of members of this genus and with a previous study using more divergent Values are MIC (mg l −" ). Strains : 1-10, see legend to genes (Grundmann et al, 2000), a cluster with A. felis, A. clevelandensis and A. felis genospecies A and a cluster with A. broomeae, A. birgiae and A. massiliensis. Conversely, the position of Afipia genospecies 3 and Afipia genospecies 3-related strains, which are different species but probably in the same genus, is impossible to assess.…”

Field Scale Evaluation of Seasonal Wastewater Treatment Efficiencies of Free Surface-Constructed Wetlands in ICRISAT, India

“…Bacteria of two genus Nitrosomonas and Nitrobacter play key role in this process 37 . Nitrosomonas bacteria first convert ammonia into nitrites; subsequently Nitrobacter bacteria convert the nitrites into nitrates 38 . The field conditions during the winter months presented near-optimal-growth condition 39 for both Nitrosomonas (pH 6.0-9.0, temperature 20-30C) and Nitrobacter (pH 7.9 and 28C).…”