High Resolution PCR Quantitation by AmpliSensor Assay

Abstract

A real-time kinetic tool for polymerase chain reaction (PCR) quantitation, AmpliSensor assay (1) quantifies PCR product by relating the rate of an amplification reaction through the progressive depletion of a rate-limiting primer. AmpliSensor assay invokes a two-step amplification scheme: 1. An initial amplification with one primer in excess in order to overproduce one strand of the target, and 2. A subsequent hemi-nested amplification for fluorescence signal detection.