High resolution packed column supercritical fluid chromatography of fatty acid methyl esters

Smith, Roger M.; Hyytiäinen, Minna; Felipe, Alejandro V.; Morris, Paul

doi:10.1002/1615-9314(20010301)24:3<208::aid-jssc208>3.0.co;2-p

Cited by 13 publications

(2 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Comprehensive two-dimensional chromatography is made possible by having different retention mechanisms in the columns of the two dimensions. When fatty acid methyl esters (FAMEs) are chromatographically separated on bare silica with neat carbon dioxide as a mobile phase, they are separated according to the number of double bonds, independent of chain length 34 . On a GC column, FAMEs are separated according to volatility or, equivalently, chain length.…”

Section: F Chromatographymentioning

confidence: 99%

A high-repetition-rate, fast temperature-programmed gas chromatograph and its online coupling to a supercritical fluid chromatograph (SFC × GC)

Malan

Walt

Rohwer

2020

Review of Scientific Instruments

View full text Add to dashboard Cite

We present a fast gas chromatographic system that can be used as a second dimension in comprehensive two-dimensional (supercritical fluid × gas) chromatography (SFC × GC). The temperature of the short (1 m long) capillary column is controlled by a resistively heated coaxial stainless-steel tube. The electrical resistance and, therefore, temperature of the stainless-steel tube are measured by continuous monitoring of the current/voltage ratio. Highly repeatable heating rates of up to 2100 °C min−1 (35 °C s−1) are obtained, which should be high enough for the most demanding fast chromatograms. To reduce the cooling time between temperature programs, the column is cooled by injecting evaporating carbon dioxide into the space between the coaxial heater and the column. This gives cooling rates of 5100 °C min−1 (85 °C s−1), which allows quick succession of temperature programs. More repeatable heating profiles with stable GC retention times together with faster cooling are significant improvements on previous SFC × GC systems. Cycle times of four gas chromatograms per minute could readily be achieved, which allows efficient coupling to high-resolution stop-flow SFC in the first dimension. We demonstrate the fast chromatograph by separating fatty acid methyl esters, yielding information that would be useful in the food and biodiesel industries.

show abstract

Section: F Chromatographymentioning

confidence: 99%

A high-repetition-rate, fast temperature-programmed gas chromatograph and its online coupling to a supercritical fluid chromatograph (SFC × GC)

Malan

Walt

Rohwer

2020

Review of Scientific Instruments

View full text Add to dashboard Cite

show abstract

“…S11d), it can be inferred that analytes with more double bonds exhibit stronger retention. 26 On the other hand, four derivatives (a, b, g, and d) of vitamin E are also examined (see ESI, † Fig. S11e).…”

mentioning

confidence: 99%