High resolution nuclear magnetic resonance studies of the conformation of luteinizing hormone releasing hormone (LH-RH) and its component peptides

1 H nuclear magnetic resonance studies of thyrotropin releasing factor (TRF)

“…No evidence for other than a random-coil configuration for TRF could be found in the spectrum. Similar findings have been reported for other small linear peptides such as pentagastrin [9] and luteinizing hormone releasing hormone [ 10]. It would appear that for these peptides where the active groups are in close proximity to each other, the hormone structure can possibly organise itself into its bound conformation during the actual binding process to the receptor.…”

Characterization of the Degradation Products of Luteinizing Hormone Releasing Hormone

“…A detailed study of the conformation of this molecule by NMR has been reported.8. 10 The highfield resonances of the His H, proton (5.7 ppm) and the His HO protons (1.05 and 2.41 pprn) are distinguishing spectral characteristics of this molecule.8J0 Chiral analysis of the sample showed that the diketopiperazine was composed of L-His and L-Trp. Final confirmation of the structural assignment was obtained by comparing the spectral properties of an authentic synthesized sample with those of the isolated sample.…”

A proton magnetic resonance study of the conformation of methionine-enkephalin as a function of pH

“…The assignments in the latter solvent mixture can be extrapolated to those in the aqueous solution (Wessels et al, 1973). The assignments in the latter solvent mixture can be extrapolated to those in the aqueous solution (Wessels et al, 1973).…”