High-resolution MRI detects cartilage swelling at the early stages of experimental osteoarthritis

Calvo, Emílio; Palacios, Itziar; Delgado, Emilio; Ruíz-Cabello, Jesús; Hernández, Paula; Sánchez‐Pernaute, Olga; Egido, Jesús; Herrero‐Beaumont, Gabriel

doi:10.1053/joca.2001.0413

Cited by 141 publications

(123 citation statements)

References 37 publications

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“…They imputed this finding to biomechanical alterations caused by the instability induced by ACLT. We have described the same findings in experimental rabbit models of osteoarthritis induced either by partial medial meniscectomy or by partial medial meniscectomy plus ACLT (5,6). Although the severity of cartilage deterioration increased when ACLT was performed in addition to meniscectomy, abnormalities followed the same anatomic distribution, being more intense in the posterior articular tibial surface.…”

Section: To the Editorsupporting

confidence: 60%

Does oral glucosamine prevent subchondral bone loss in an animal model of osteoarthritis? Comment on the article by Wang et al

Castañeda¹,

Largo²,

Calvo³

et al. 2008

Arthritis & Rheumatism

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Section: To the Editorsupporting

confidence: 60%

Does oral glucosamine prevent subchondral bone loss in an animal model of osteoarthritis? Comment on the article by Wang et al

Castañeda¹,

Largo²,

Calvo³

et al. 2008

Arthritis & Rheumatism

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“…network of cartilage is a prerequisite for the swelling and increased water content of joint cartilage, observed soon after injury in experimental OA and cartilage injury models (7,(55)(56)(57). The increased release of crosslinked CTX-II to SF shortly after joint injury, as shown in this study, may reflect such critical damage to the 3-dimensional collagen network.…”

Section: Discussionmentioning

confidence: 51%

The release of crosslinked peptides from type II collagen into human synovial fluid is increased soon after joint injury and in osteoarthritis

Lohmander¹,

Atley²,

Pietka³

et al. 2003

Arthritis & Rheumatism

234

165

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Objective. To determine concentrations of crosslinked C-telopeptide fragments of type II collagen (CTX-II) in synovial fluid (SF) from patients with joint injury, osteoarthritis (OA), or other knee arthritides.Methods. Two study groups were used: a crosssectional group, which included healthy-knee volunteers (reference group [REF]) and patients with pseudogout (PPA), an anterior cruciate ligament tear with or without a meniscus tear (INJ), or primary knee OA (POA); and a longitudinal group, which included patients with arthroscopic cartilage changes or septic arthritis. CTX-II was quantified by competition enzyme-linked immunosorbent assay (ELISA) based on a monoclonal antibody that recognized the C-terminus of the peptide EKGPDP as a proteolytic neoepitope. Aggrecan fragments, matrix metalloproteinases 1 and 3, and tissue inhibitor of metalloproteinases 1 were determined by ELISAs.Results. Concentrations of CTX-II in SF were higher in patients with PPA, INJ, and POA than in the REF group (P < 0.001). After joint injury, mean levels of CTX-II in SF were increased above REF levels at all time intervals (P < 0.001), and were highest within hours after trauma. In those in the longitudinal study group with joint cartilage damage, variation coefficients for CTX-II were 81% (between patients) and 64% (within patient), monitored over 1 year. In a patient with septic arthritis, SF CTX-II increased at the onset of symptoms, and peaked 30-fold higher than the baseline. Concentrations of all biomarkers decreased with successful treatment.Conclusion. This is the first report to describe the release into SF of soluble molecular fragments specific for the degradation of mature, crosslinked, type II collagen (CII) in human OA and joint injury. The results provide strong evidence that the integrity of the CII network of cartilage is compromised soon after joint injury and in arthritis. This early degradation of CII may represent an important treatment target.Osteoarthritis (OA) and joint injury are characterized by remodeling and degradation of cartilage, bone, and other joint tissues. The normally very slow turnover rate of cartilage matrix (1) is increased, as observed by several techniques in both animal OA models and human OA. Phasic changes in both synthesis and degradation of collagen and other matrix molecules (2-6) are associated with altered tissue structure and material properties (7-9).Increased joint tissue turnover after injury and in OA can be detected by a release of molecules and molecular fragments into synovial fluid (SF), blood, and urine. For example, the stimulated synthesis of type II collagen (CII) results in higher levels of CII C-propeptide in SF (10,11). Stimulated aggrecan synthesis results in more aggrecan fragments carrying the 846 epitope (12). Higher synovial concentrations of matrix metalloproteinases (MMPs), such as MMP-1 and MMP-3, and proteinase activity after joint injury and in OA are consistent with the observed expression of these

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“…Several studies on animals of early OA Figure 5 Micro-CT 3D models of a representative sample of each experimental group. Cartilage swelling characteristic of the first stages of OA can be clearly appreciated in OA samples, whereas diacerein-treated samples recover normal cartilage morphology like CTRL with magnetic resonance imaging and histomorphometry identified an initial phase of cartilage hypertrophy prior to its degeneration and loss [35,36]. The explanation has not been found yet, and it is not known whether it is the tissue expression to an inflammatory and repair phenomenon or if it represents a permanent damage of the joint tissue.…”

Section: Discussionmentioning

confidence: 95%

Effects of diacerein on cartilage and subchondral bone in early stages of osteoarthritis in a rabbit model

Permuy

López

Miño

et al. 2012

Bone

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Background: Osteoarthritis is thought to be the most prevalent chronic and disabling joint disease in animals and humans. At present, there is no ideal treatment option. The aim of this study was to assess the effects of the treatment with oral diacerein on articular cartilage, synovial membrane and subchondral bone in an experimental rabbit model of osteoarthritis by micro-CT evaluation and histological analysis. To this purpose, osteoarthritis was surgically induced on one knee of 16 rabbits using the contralateral knee as healthy controls. Treatment was started three weeks later and lasted eight weeks. Animals were divided into two groups for treatment: Placebo (treated daily with oral saline) and diacerein (treated orally with 1.5 mg/kg/day of diacerein).

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High-resolution MRI detects cartilage swelling at the early stages of experimental osteoarthritis

Cited by 141 publications

References 37 publications

Does oral glucosamine prevent subchondral bone loss in an animal model of osteoarthritis? Comment on the article by Wang et al

Does oral glucosamine prevent subchondral bone loss in an animal model of osteoarthritis? Comment on the article by Wang et al

The release of crosslinked peptides from type II collagen into human synovial fluid is increased soon after joint injury and in osteoarthritis

Effects of diacerein on cartilage and subchondral bone in early stages of osteoarthritis in a rabbit model

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