High-resolution morphometric analysis of human osteoblastic cell adhesion on clinically relevant orthopedic alloys

Shah, Asist K.; Sinha, Raj; Hickok, Noreen J.; Tuan, Rocky S.

doi:10.1016/s8756-3282(99)00077-0

Cited by 83 publications

(68 citation statements)

References 42 publications

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“…2 is not frequently measured, especially the early attachment phase characterized by t 1/2 and %I max, because cell-enumeration protocols are quite labor intensive (there are a variety of cell-enumeration methods available including dye techniques [67][68][69][70][71][72][73][74], autoradiography [75], light and electron microscopy [76][77][78], Coulter counting [79], hemocytometry [80], spectrometry [81,82], nuclei number [83], total DNA [84], total protein concentration [78,85] that may or may not give similar results, depending on cell number and specific experimental conditions). Instead, a variety of experimental shortcuts are taken, such as measuring attached-cell-number after some arbitrary cell-surface contact time [86][87][88][89].…”

Section: Cell Attachment and Proliferation Kineticsmentioning

confidence: 99%

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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Time-dependent phenotypic response of a model osteoblast cell line (hFOB 1.19, ATCC, CRL-11372) to substrata with varying surface chemistry and topography is reviewed within the context of extant cell-adhesion theory. Cell-attachment and proliferation kinetics are compared using morphology as a leading indicator of cell phenotype. Expression of (α 2 , α 3 , α 4 , α 5 , α v , β 1 and β 3 ) integrins, vinculin, as well as secretion of osteopontin and type I collagen supplement this visual assessment of hFOB growth. It is concluded that significant cell-adhesion events -contact, attachment, spreading, and proliferation -are similar on all surfaces, independent of substratum surface chemistry/energy. However, this sequence of events is significantly delayed and attenuated on hydrophobic (poorly water-wettable) surfaces exhibiting characteristically low-attachment efficiency and long induction periods before cells engage in an exponential-growth phase. Results suggest that a 'time-cell-substratum compatibility-superposition-principle' is at work wherein similar bioadhesive outcomes can be ultimately achieved on all surface types with varying hydrophilicity, but the time required to arrive at this outcome increases with decreasing cellsubstratum compatibility. Genomic and proteomic tools offer unprecedented opportunity to directly measure changes in the cellular machinery that lead to observed cell responses to different materials. But for the purpose of measuring structure-property relationships that can guide biomaterial development, genomic/proteomic tools should be applied early in the adhesion/spreading process before cells have an opportunity to significantly remodel the cell-substratum interface, effectively erasing cause-and-effect relationships between cell cell-substratum compatibility and substratum properties.Impact Statement-This review quantifies relationships among cell phenotype, substratum surface chemistry/energy, topography, and cell-substratum contact time for the model osteoblast cell

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Section: Cell Attachment and Proliferation Kineticsmentioning

confidence: 99%

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

et al. 2007

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“…Specifically, we have compared the performance of two basal media, Dulbecco's modified Eagle's medium (DMEM), widely used for the culture of various cell types including bone marrow-derived MPCs [14,18,19], and DMEM with Kaighn's modification of Ham's F12 (F12K) supplemented with ascorbic acid and L-glutamine, which has been routinely used in studies involving cells derived from trabecular bone [9,20,21].…”

Section: ® Original Articlementioning

confidence: 99%

Characterization of Multipotential Mesenchymal Progenitor Cells Derived from Human Trabecular Bone

et al. 2003

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ABSTRACT-cell population resident within collagenase-treated, culture-processed bone fragments, which upon migration established a homogeneous population of MPCs. Additionally, we have introduced a system of culturing these MPCs that best supports and maintains their optimal differentiation potential during long-term culture expansion. When cultured as described, the trabecular bone-derived cells display stem cell-like capabilities, characterized by a stable undifferentiated phenotype as well as the ability to proliferate extensively while retaining the potential to differentiate along the osteoblastic, adipocytic, and chondrocytic lineages, even when maintained in long-term in vitro culture.

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“…It is known that cells with spherical geometry present low adhesion to the substrate 31 and high occurrence of this geometry means that the substrate is not favorable for cell growth. Thus, we define the form factor F f given by equation …”

Section: A Cell Culturementioning

confidence: 99%

Cell adhesion and growth on surfaces modified by plasma and ion implantation

Araujo

Teixeira

Silva

et al. 2014

Journal of Applied Physics

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In this study, we show and discuss the results of the interaction of living CHO (Chinese Hamster Ovary) cells, in terms of adhesion and growth on glass, SU-8 (epoxi photoresist), PDMS (polydimethylsiloxane), and DLC (hydrogen free diamond-like carbon) surfaces. Glass, SU-8, and DLC but not PDMS showed to be good surfaces for cell growth. DLC surfaces were treated by oxygen plasma (DLC-O) and sulfur hexafluoride plasma (DLC-F). After 24 h of cell culture, the number of cells on DLC-O was higher than on DLC-F surface. SU-8 with silver implanted, creating nanoparticles 12 nm below the surface, increased significantly the number of cells per unit area. V C 2014 AIP Publishing LLC. [http://dx

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High-resolution morphometric analysis of human osteoblastic cell adhesion on clinically relevant orthopedic alloys

Cited by 83 publications

References 42 publications

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro☆

Characterization of Multipotential Mesenchymal Progenitor Cells Derived from Human Trabecular Bone

Cell adhesion and growth on surfaces modified by plasma and ion implantation

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