High-Resolution Micropatterned Teflon AF Substrates for Biocompatible Nanofluidic Devices

Czolkos, Ilja; Hakonen, Bodil; Orwar, Owe; Jesorka, Aldo

doi:10.1021/la2044784

Cited by 15 publications

(17 citation statements)

References 29 publications

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“…In contrast, repulsive forces in the hydrophobic chains and headgroups will be generated from the steric, hydration, and electrostatic effects when lipid molecules in the shape of inverted cones form SLBs, giving rise to an imbalance of the lipid lateral pressure and elevated bending energy at the molecular level. However, on hydrophobic substrates (e.g., alkanethiol-coated gold, fluoropolymers [38], and the epoxy SU-8 [39]), µ sm µ sb , inducing the formation of a supported lipid monolayer. This simple estimation of the Gibbs free energy indicates that SLBs form on hydrophilic surfaces and lipid monolayers form on hydrophobic surfaces.…”

Section: Salb Formation-estimation Of Energetics and A Thermodynamics Model 21 Estimation Of Energetics Of Salb Formationmentioning

confidence: 99%

Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

Tae

Cho

et al. 2022

Micromachines

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The solvent-assisted lipid bilayer (SALB) formation method provides a simple and efficient, microfluidic-based strategy to fabricate supported lipid bilayers (SLBs) with rich compositional diversity on a wide range of solid supports. While various studies have been performed to characterize SLBs formed using the SALB method, relatively limited work has been carried out to understand the underlying mechanisms of SALB formation under various experimental conditions. Through thermodynamic modeling, we studied the experimental parameters that affect the SALB formation process, including substrate surface properties, initial lipid concentration, and temperature. It was found that all the parameters are critically important to successfully form high-quality SLBs. The model also helps to identify the range of parameter space within which conformal, homogeneous SLBs can be fabricated, and provides mechanistic guidance to optimize experimental conditions for lipid membrane-related applications.

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Section: Salb Formation-estimation Of Energetics and A Thermodynamics Model 21 Estimation Of Energetics Of Salb Formationmentioning

confidence: 99%

Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

Tae

Cho

et al. 2022

Micromachines

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“…From a perspective in structural and material design, we believe that there are plenty of opportunities to control particle aggregation, droplet movement, or surface wettability depending on the types of solutions or solutes. For example, if our structural design for a robust particle capture would be incorporated with advanced surface engineering technologies for implementing superhydrophobic or slippery omniphobic surfaces, − then we expect that the large aggregates formed due to the presence of biological particles could be concentrated into smaller one. This leads to more efficient particle capture in the microcavity structures.…”

Section: Discussionmentioning

confidence: 99%

Microstructured Surfaces for Reducing Chances of Fomite Transmission via Virus-Containing Respiratory Droplets

Kim

Nam

et al. 2021

ACS Nano

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Evaporation-induced particle aggregation in drying droplets is of significant importance in the prevention of pathogen transfer due to the possibility of indirect fomite transmission of the infectious virus particles. In this study, particle aggregation was directionally controlled using contact line dynamics (pinned or slipping) and geometrical gradients on microstructured surfaces by the systematic investigation of the evaporation process on sessile droplets and sprayed microdroplets laden with virus-simulant nanoparticles. Using this mechanism, we designed robust particle capture surfaces by significantly inhibiting the contact transfer of particles from fomite surfaces. For the proof-of-concept, interconnected hexagonal and inverted pyramidal microwall were fabricated using ultravioletbased nanoimprint lithography, which is considered to be a promising scalable manufacturing process. We demonstrated the potentials of an engineered microcavity surface to limit the contact transfer of particle aggregates deposited with the evaporation of microdroplets by 93% for hexagonal microwall and by 96% for inverted pyramidal microwall. The particle capture potential of the interconnected microstructures was also investigated using biological particles, including adenoviruses and lung-derived extracellular vesicles. The findings indicate that the proposed microstructured surfaces can reduce the indirect fomite transmission of highly infectious agents, including norovirus, rotavirus, or SARS-CoV-2, via respiratory droplets.

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“…In general, high-energy surfaces, such as glass and various metal oxides, are associated with single and double bilayers, whereas low-energy surfaces, such as hydrophobic polymers, support monolayer films. 7 Although single bilayer membranes have been most abundantly employed due to their structural similarity to the plasma membrane, there is currently a growing interest in fabrication of monolayer 11 and double bilayer films, 9,10 which feature different properties and capabilities in various application contexts.…”

Section: ■ Introductionmentioning

confidence: 99%

Molecular Lipid Films on Microengineering Materials

et al. 2019

Self Cite

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In this study, we have systematically investigated the formation of molecular phospholipid films on a variety of solid substrates fabricated from typical surface engineering materials and the fluidic properties of the lipid membranes formed on these substrates. The surface materials comprise of borosilicate glass, mica, SiO2, Al (native oxide), Al2O3, TiO2, ITO, SiC, Au, Teflon AF, SU-8, and graphene. We deposited the lipid films from small unilamellar vesicles (SUVs) by means of an open-space microfluidic device, observed the formation and development of the films by laser scanning confocal microscopy, and evaluated the mode and degree of coverage, fluidity, and integrity. In addition to previously established mechanisms of lipid membrane–surface interaction upon bulk addition of SUVs on solid supports, we observed nontrivial lipid adhesion phenomena, including reverse rolling of spreading bilayers, spontaneous nucleation and growth of multilamellar vesicles, and the formation of intact circular patches of double lipid bilayer membranes. Our findings allow for accurate prediction of membrane–surface interactions in microfabricated devices and experimental environments where model membranes are used as functional biomimetic coatings.

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High-Resolution Micropatterned Teflon AF Substrates for Biocompatible Nanofluidic Devices

Cited by 15 publications

References 29 publications

Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

Thermodynamic Modeling of Solvent-Assisted Lipid Bilayer Formation Process

Microstructured Surfaces for Reducing Chances of Fomite Transmission via Virus-Containing Respiratory Droplets

Molecular Lipid Films on Microengineering Materials

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