High resolution mass spectrometry provides novel insights into the ganglioside pattern of brain cavernous hemangioma

Ica, Raluca; Simulescu, Anca; Sârbu, Mirela; Munteanu, Cristian V. A.; Vukelić, Željka; Zamfir, Alina D.

doi:10.1016/j.ab.2020.113976

Cited by 7 publications

(17 citation statements)

References 34 publications

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“…This form, characterized by cavities filled with blood with a very thin vascular wall, is specific to the frontal and temporal lobes [ 169 ], affects 0.5% of the population of all ages, becomes symptomatic only in 40% of cases, and occurs in the third to sixth decade of life [ 170 ]. Most frequently, cavernous hemangioma is accidentally diagnosed during imaging investigations, such as CT scan, MRI, nuclear magnetic resonance (NMR) or ultrasonography (ultrasound) preformed following headaches, convulsions, or neurological deficits reported by the patients [ 171 ]. Of extreme importance for the clinical management of hemangioma is its early detection, at an incipient and resectable stage, based on routine screening and cancer biomarker detection prior to the clinical symptoms [ 163 , 171 ].…”

Section: Primary Brain Tumorsmentioning

confidence: 99%

“…Most frequently, cavernous hemangioma is accidentally diagnosed during imaging investigations, such as CT scan, MRI, nuclear magnetic resonance (NMR) or ultrasonography (ultrasound) preformed following headaches, convulsions, or neurological deficits reported by the patients [ 171 ]. Of extreme importance for the clinical management of hemangioma is its early detection, at an incipient and resectable stage, based on routine screening and cancer biomarker detection prior to the clinical symptoms [ 163 , 171 ].…”

Section: Primary Brain Tumorsmentioning

confidence: 99%

“…A recent revisit of human brain hemangioma (HE42) GGs using an approach based on high-resolution MS on an Orbitrap MS with nanoESI tuned for detection in the negative ion mode [ 171 ] revealed a highly complex molecular ion pattern, encompassing species characterized by a prominent diversity of both glycan chains and ceramide moiety compositions, as well as various biologically significant modifications, such as O -acetylation, O -fucosylation, and CH 3 COO − attachments ( Figure 4 ).…”

Section: Primary Brain Tumorsmentioning

confidence: 99%

“…Moreover, the increased incidence of glycoforms characterized by a low degree of sialylation and short glycan chains, are in agreement with previously achieved data [ 163 ] related to the reduced overall sialylation in hemangioma. In view of the elevated incidence of GD3 glycoforms in HE42, the structural analysis by CID MS/MS provided data able to confirm the GD3(d18:1/24:1) and GD3(d18:1/24:2) structures, which might have a potential biomarker role and could be used in early diagnosis [ 171 ].…”

Section: Primary Brain Tumorsmentioning

confidence: 99%

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Gangliosides as Biomarkers of Human Brain Diseases: Trends in Discovery and Characterization by High-Performance Mass Spectrometry

Sârbu

Ica

Zamfir

2022

IJMS

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Gangliosides are effective biochemical markers of brain pathologies, being also in the focus of research as potential therapeutic targets. Accurate brain ganglioside mapping is an essential requirement for correlating the specificity of their composition with a certain pathological state and establishing a well-defined set of biomarkers. Among all bioanalytical methods conceived for this purpose, mass spectrometry (MS) has developed into one of the most valuable, due to the wealth and consistency of structural information provided. In this context, the present article reviews the achievements of MS in discovery and structural analysis of gangliosides associated with severe brain pathologies. The first part is dedicated to the contributions of MS in the assessment of ganglioside composition and role in the specific neurodegenerative disorders: Alzheimer’s and Parkinson’s diseases. A large subsequent section is devoted to cephalic disorders (CD), with an emphasis on the MS of gangliosides in anencephaly, the most common and severe disease in the CD spectrum. The last part is focused on the major accomplishments of MS-based methods in the discovery of ganglioside species, which are associated with primary and secondary brain tumors and may either facilitate an early diagnosis or represent target molecules for immunotherapy oriented against brain cancers.

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Section: Primary Brain Tumorsmentioning

confidence: 99%

Section: Primary Brain Tumorsmentioning

confidence: 99%

Section: Primary Brain Tumorsmentioning

confidence: 99%

Section: Primary Brain Tumorsmentioning

confidence: 99%

See 2 more Smart Citations

Gangliosides as Biomarkers of Human Brain Diseases: Trends in Discovery and Characterization by High-Performance Mass Spectrometry

Sârbu

Ica

Zamfir

2022

IJMS

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“…In human brain, the expression and structure of gangliosides exhibits a well-defined regional specificity which changes, also in a specific way, with age 16 and when the respective brain area is affected by neuropathologies including genetic disorders, neurodegenerative diseases, benign or malignant tumors. 17 − 20…”

Section: Introductionmentioning

confidence: 99%

High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex

Ica

Munteanu

Vukelić

et al. 2021

Eur J Mass Spectrom (Chichester)

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We have developed here a superior methodology based on high-resolution mass spectrometry for screening and fragmentation analysis of gangliosides extracted and purified from the human motor cortex . The experiments, conducted on a nanoelectrospray Orbitrap mass spectroscope in the negative ion mode, allowed the discrimination in the native mixture extracted from human motor cortex of no less than 83 different gangliosides, which represents the highest number of structures identified so far in this brain region. The spectral data, acquired in high-resolution mass spectrometry mode with a remarkable sensitivity and an average mass accuracy of 4.48 ppm, also show that the gangliosidome of motor cortex is generally characterized by species exhibiting a much higher degree of sialylation than previously known. Motor cortex was found dominated by complex structures with a sialylation degree ≥3, exhibiting long saccharide chains, in the G1 class. Fucogangliosides and species with the glycan chain elongated by either O-acetylation and/or acetate anion attachments were also detected; the later modification was for the first time discovered in this brain region. Of major significance is the identification of hepta and octasialylated species of GS1 and GO1 type, which are among the structures with the longest oligosaccharide chain discovered so far in the human brain. In the last stage of research, tandem mass spectrometry performed by higher energy collision dissociation provided structural data documenting the occurrence of GT1b (d18:1/20:0) isomer in the human motor cortex.

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Analysis of glycosphingolipids in the central nervous system

Guo

2024

Glycosphingolipids in the Central Nervous System

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High resolution mass spectrometry provides novel insights into the ganglioside pattern of brain cavernous hemangioma

Cited by 7 publications

References 34 publications

Gangliosides as Biomarkers of Human Brain Diseases: Trends in Discovery and Characterization by High-Performance Mass Spectrometry

Gangliosides as Biomarkers of Human Brain Diseases: Trends in Discovery and Characterization by High-Performance Mass Spectrometry

High-resolution mass spectrometry reveals a complex ganglioside pattern and novel polysialylated structures associated with the human motor cortex

Analysis of glycosphingolipids in the central nervous system

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