High-Resolution Mass Spectra of Compounds Emerging from a Gas Chromatograph.

Watson, J. Throck; Biemann, K.

doi:10.1021/ac60212a001

Cited by 183 publications

(47 citation statements)

References 14 publications

(11 reference statements)

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“…This problem was finally solved in a practical way simultaneously by Ragnar Ryhage [26] and in our laboratory [27]. The former used the diffusion principle, a jet orifice in front of an aperture (modeled after processes previously used for the separation of uranium isotopes as their hexafluorides), while we used the faster effusion of small molecules (helium) over large molecules through the wall of a tube of fritted glass.…”

Section: Gas Chromatographymentioning

confidence: 99%

“…By that time we had continuously improved the methodology through the replacement of acetylation with trifluoroacetylation [34] (second step of Scheme 3) and O-trimethylsilylation [35] to increase volatility, which extended applicability to these larger peptides. Direct coupling of the gas chromatograph with the mass spectrometer [27] made it possible to identify 61 peptides in the hydrolyzate of monellin-I in a single experiment.…”

Section: Peptides and Proteinsmentioning

confidence: 99%

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Four decades of structure determination by mass spectrometry: From alkaloids to heparin

Biemann

2002

J. Am. Soc. Mass Spectrom.

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The early (1950's and 1960's) use of mass spectrometry in natural products chemistry and its evolution to the present significance in biochemistry is recounted. This methodology allowed the facile and speedy determination of the structure of a number of indole alkaloids, such as sarpagine, quebrachamine, and two groups isolated from the roots of Aspidosperma quebracho blanco. At the same time, the first strategy for the sequencing of small peptides by mass spectrometry was demonstrated. It slowly advanced, over a period of two decades, to an important alternative of the ubiquitous automated Edman degradation. Further advances in methodology and instrumentation established mass spectrometry as today's indispensable tool for the characterization of proteins in biochemistry and biology. A new concept of the ionization of highly acidic compounds as the protonated complexes with basic peptides, which allows the accurate determination of the molecular weights of the former, a highly sensitive method for the sequencing of heparin fragments and related sulfated glycosaminoglycans was developed more recently. (J Am Soc Mass Spectrom 2002, 13, 1254-1272

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Section: Gas Chromatographymentioning

confidence: 99%

Section: Peptides and Proteinsmentioning

confidence: 99%

Four decades of structure determination by mass spectrometry: From alkaloids to heparin

Biemann

2002

J. Am. Soc. Mass Spectrom.

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“…The GC-MS interface was invented in the 1960's to provide a sensitive detector for the GC and to provide a sample introduction system for the MS (James, 1970;Ryhage, 1964;Watson & Biemann, 1964). Samples injected into the GC are vaporized and separated by boiling point and by differential affinity for the stationary phase contained in a long column.…”

Section: Gas Chromatography-mass Spectrometry (Gc-ms)mentioning

confidence: 99%

Stable isotopes in obesity research

Dolnikowski

Marsh

Das

et al. 2004

Mass Spectrometry Reviews

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Obesity is recognized as a major public health problem. Obesity is a multifactorial disease and is often associated with a wide range of comorbidities including hypertension, non-insulin dependent (Type II) diabetes mellitus, and cardiovascular disease, all of which contribute to morbidity and mortality. This review deals with stable isotope mass spectrometric methods and the application of stable isotopes to metabolic studies of obesity. Body composition and total energy expenditure (TEE) can be measured by mass spectrometry using stable isotope labeled water, and the metabolism of protein, lipid, and carbohydrate can be measured using appropriate labeled tracer molecules.

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“…each. WATSON and BIEMANN (1964) obtained high-resolution mass spectra of compounds emerging from agas chromatograph by using an all-glass, valveless pressure reduction system to feed the efRuent continuously. A tenfold or better enrichment of the gas stream was obtained by using a fritted tube as a stream splitter; the helium carrier gas diffused through the frit much more rapidly than the larger molecules.…”

Section: E) Spectrometric Detectorsmentioning

confidence: 99%

Residue Reviews / Rückstands-Berichte

1967

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High-Resolution Mass Spectra of Compounds Emerging from a Gas Chromatograph.

Cited by 183 publications

References 14 publications

Four decades of structure determination by mass spectrometry: From alkaloids to heparin

Four decades of structure determination by mass spectrometry: From alkaloids to heparin

Stable isotopes in obesity research

Residue Reviews / Rückstands-Berichte

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