High-resolution mapping of a linkage group on mouse chromosome 8 conserved on human chromosome 16Q

Abstract

We have performed a high-resolution linkage analysis for the conserved segment on distal mouse Chromosome (Chr) 8 that is homologous to human Chr 16q. The interspecific backcross used involved M. m. molossinus and an M. m. domesticus line congenic for an M. spretus segment from Chr 8 flanked by phenotypic markers Os (oligosyndactyly) and e, a coat colormarker. From a total of 682 N2 progeny, the 191 animals revealing a recombination event between these phenotypic markers were typed for 23 internal loci. The following locus order with distances in cM was obtained: (centromere)-Os-4.1-Mmp2-0.2-Ces1,Es1, Es22-1.2-Mt1,D8Mit15-2.2-Got2, D8Mit11-3.7-Es30-0.3-Es2, Es7-0.9-Ctra1,Lcat-0.3-Cdh1, Cadp, Nmor1, D8Mit12-0.2-Mov34-2.5-Hp,Tat-0.2-Zfp4-1.6-Zfp1,+ ++Ctrb-10.9-e. In a separate interspecific cross involving 62 meioses, Dpep1 was mapped together with Aprt and Cdh3 at 12.9 cM distal to Hp, Tat, to the vicinity of e. Our data give locus order for markers not previously resolved, add Mmp2 and Dpep1 as new markers on mouse Chr 8, and indicate that Ctra1 is the mouse homolog for human CTRL. Comparison of the order of 17 mouse loci with that of their human homologs reveals that locus order is well conserved and that the conserved segment in the human apparently spans the whole long arm of Chr 16.

The Butyrylcholinesterase Knockout Mouse as a Model for Human Butyrylcholinesterase Deficiency

“…The characteristics of the butyrylthiocholine hydrolase were consistent with the interpretation that this activity was due to a carboxylesterase. Mice have 16 carboxylesterases encoded by two gene clusters on chromosome 8 (Ronai et al, 1993;Becker-Follmann et al, 1997). A graphic view of the carboxylesterase gene clusters, and the links to the gene and protein sequences, can be found at www.ncbi.nlm.nih.gov/ mapview by selecting Mus musculus Build 36 and searching for carboxylesterase, all matches.…”

Genetic Predisposition to 4NQO-Induced Tongue Carcinogenesis in the Rat

“…A precisely mapped location of NQO1 in the rat has not been reported, but it is in the syntenic regions of the human chromosome 16q22.1 and the mouse chromosome 8 [26][27][28] . Quinone oxidoreductase is one of the major enzymes that convert 4NQO to the more active metabolite, 4-hydroxyaminoquinoline 1-oxide [25,27] . Recently, we clarifi ed that the locus of NQO1 is co-mapped with Tscc1 between D19Mit9 and D19wox8 [29] .…”