High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) as a tool in the determination of biomarkers of Passiflora-based herbal medicines

Flores, Igor S.; Martinelli, Bruno C.B.; Lião, Luciano M.

doi:10.1016/j.fitote.2020.104500

Cited by 10 publications

(5 citation statements)

References 14 publications

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“…Compounds 2 and 5 had never been reported before from plants of the genus Achillea. The remaining eight compounds (Figure 2) were phenolic derivatives, identified as chlorogenic acid (10) [19], the polymethoxylated flavonols penduletin (11) [20] and artemetin (12) [21], the polymethoxylated flavones santoflavone (13) [22] and salvigenin ( 14) [23] and the flavone-C-glycosides vitexin (15) [24], isovitexin ( 16) [25], and isoschaftoside (17) [26]. Wilhelmsin (7) was isolated as an optically active colorless amorphous solid with molecular formula C19H25NO6, as determined by HR-ESIMS.…”

Section: Chemical Analysis and Structure Elucidation Of Wilhelmsin And Wilhelmsolidementioning

confidence: 99%

Salvigenin, a Trimethoxylated Flavone from Achillea Wilhelmsii C. Koch, Exerts Combined Lipid-Lowering and Mitochondrial Stimulatory Effects

et al. 2021

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Phytochemical analysis of the Iranian plant Achillea wilhelmsii led to the isolation of 17 pure secondary metabolites belonging to the classes of sesquiterpenoids and phenolics. Two of these compounds, named wilhemsin (7) and wilhelmsolide (9), are new sesquiterpenoids, and the first shows undescribed structural features. Their structures were elucidated through extensive spectroscopic analysis, mainly based on 1D and 2D NMR, and chemical derivatization. Starting from plant traditional use and previous reports on the activity of the plant extracts, all the pure compounds were evaluated on endpoints related to the treatment of metabolic syndrome. The sesquiterpene hanphyllin (8) showed a selective cholesterol-lowering activity (−12.7% at 30 µM), santoflavone (13) stimulated glucose uptake via the GLUT transporter (+16.2% at 30 µM), while the trimethoxylated flavone salvigenin (14) showed a dual activity in decreasing lipid levels (−22.5% palmitic acid biosynthesis at 30 µM) and stimulating mitochondrial functionality (+15.4% at 30 µM). This study further confirms that, in addition to the antioxidants vitexin, isovitexin, and isoschaftoside, A. wilhelmsii extracts contain molecules that can act at different levels on the metabolic syndrome symptoms.

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Section: Chemical Analysis and Structure Elucidation Of Wilhelmsin And Wilhelmsolidementioning

confidence: 99%

Salvigenin, a Trimethoxylated Flavone from Achillea Wilhelmsii C. Koch, Exerts Combined Lipid-Lowering and Mitochondrial Stimulatory Effects

et al. 2021

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“…Commonly employed analytical techniques for the quantification of DZP and related benzodiazepines involve the integration of highperformance liquid chromatography with UV detection [7][8][9][10] or diode array detector 11 as well as gas chromatography with mass spectrometry. [12][13][14][15] Other methodologies, including capillary electrophoresis, [16][17][18] nuclear magnetic resonance 19,20 and immunoassay methods, [21][22][23] have also been documented for DZP sensing in the scientific literature. Furthermore, the investigative study documents various electroanalytical techniques dedicated to the determination of DZP or other benzodiazepines, including polarography, [24][25][26] voltammetry [27][28][29][30][31] and potentiometry.…”

Section: Diazepam (Dzp) Predominantly Accessible Under the Trade Namementioning

confidence: 99%

Powerful Analytical Platform for Diazepam Determination in Pharmaceuticals and Alcoholic Drinks Based on Batch Injection Analysis Coupled with Adsorptive Stripping Voltammetry

Haššo,

Kekeľáková,

Hanko

et al. 2024

J. Electrochem. Soc.

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The presented study focuses on the development and optimization of a powerful electroanalytical platform for the direct quantification of diazepam (DZP). This innovative approach integrates a batch injection analysis (BIA) system with a screen-printed electrode arrangement employing square-wave adsorptive stripping voltammetry (SWAdSV). The BIA-SWAdSV method underwent a comprehensive evaluation, wherein various experimental and instrumental parameters were systematically examined in detail. Beneficial analytical performance for detecting DZP was attained in Britton-Robinson buffer with pH 6.0, with an amplitude of 75 mV, a frequency of 10 Hz, a deposition potential of –1.2 V, a deposition time of 150 s, an injection volume of 75 µL, a dispensing rate of 7 µL/s, and without stirring during the deposition step. Under these conditions, the proposed BIA-SWAdSV method demonstrated an adequately broad linear concentration range from 5 to 40 µM (R2 = 0.997) with a micromolar limit of detection of (2 µM) and a satisfactory precision (RSD = 5%). The practical applicability of the newly-established and powerful analytical protocol was confirmed through the analysis of pharmaceuticals and a "fortified" sample of an alcoholic drink (rum) associated with potential criminal activities involving DZP abuse.

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“…This is helpful when only one class has a medical application as in the case of Trichilia catigua [70] or Withania somnifera [71]. It can also help to distinguish between different cultivars of apples [72], melons [73], rice [74], persimmons [75], ginseng [76], almonds [77] and curtis [78]. Table 3.…”

Section: Applications Of Hr-mas Nmr In Plant Metabolomicsmentioning

confidence: 99%

HR-MAS NMR Applications in Plant Metabolomics

2021

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Metabolomics is used to reduce the complexity of plants and to understand the underlying pathways of the plant phenotype. The metabolic profile of plants can be obtained by mass spectrometry or liquid-state NMR. The extraction of metabolites from the sample is necessary for both techniques to obtain the metabolic profile. This extraction step can be eliminated by making use of high-resolution magic angle spinning (HR-MAS) NMR. In this review, an HR-MAS NMR-based workflow is described in more detail, including used pulse sequences in metabolomics. The pre-processing steps of one-dimensional HR-MAS NMR spectra are presented, including spectral alignment, baseline correction, bucketing, normalisation and scaling procedures. We also highlight some of the models which can be used to perform multivariate analysis on the HR-MAS NMR spectra. Finally, applications of HR-MAS NMR in plant metabolomics are described and show that HR-MAS NMR is a powerful tool for plant metabolomics studies.

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High-resolution magic angle spinning nuclear magnetic resonance (HR-MAS NMR) as a tool in the determination of biomarkers of Passiflora-based herbal medicines

Cited by 10 publications

References 14 publications

Salvigenin, a Trimethoxylated Flavone from Achillea Wilhelmsii C. Koch, Exerts Combined Lipid-Lowering and Mitochondrial Stimulatory Effects

Salvigenin, a Trimethoxylated Flavone from Achillea Wilhelmsii C. Koch, Exerts Combined Lipid-Lowering and Mitochondrial Stimulatory Effects

Powerful Analytical Platform for Diazepam Determination in Pharmaceuticals and Alcoholic Drinks Based on Batch Injection Analysis Coupled with Adsorptive Stripping Voltammetry

HR-MAS NMR Applications in Plant Metabolomics

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