High resolution magic angle spinning 1H nuclear magnetic resonance analysis of intact prostatic hyperplastic and tumour tissues

Abstract

High resolution magic angle spinning (MAS) 1 H nuclear magnetic resonance (NMR) spectra of whole pieces of human prostatic tissue ( < 40 mg per sample) from patients with benign prostatic hyperplasia (BPH) and malignant tumours have been obtained and compared with high resolution NMR solution state spectra obtained via a standard tissue extraction method. Well resolved MAS-NMR spectra of the tissue samples were obtained in which both cytosolic and membrane-related compounds could be identified. Two-dimensional 1 H MAS-NMR measurements, including J-resolved and shift correlation spectra, were also obtained to allow metabolite signal assignment. In all cases high quality spectra were obtained that were rich in bioanalytical information on a wide range of intracellular metabolites and lipids. Marked differences were observed between MAS spectra from tumours versus BPH tissue, particularly in the lipid signals, which were much more intense in tumours. This was in contrast to the protein-free extracts of the same samples which showed much more similar NMR profiles for the two disease classes. These data indicate that MAS-NMR spectra of intact tissue biopsy samples give a wider range of bioanalytical information on small and large molecules than conventional, more labour intensive cell extraction procedures, and this strengthens the case for the use of MAS-NMR in clinical diagnostics.

Peer Reviewed: So What’s the Deal with Metabonomics?

“…Typical high-resolution 1 H MAS NMR spectra of a range of tissues are shown in Figure 3. Such experiments indicate that the metabolic profiles of diseased or toxin-affected tissues are substantially different from those of healthy organs (10,11). In addition, MAS NMR can access information regarding the compartmentalization of metabolites within cellular environments.…”

High-Resolution Magic Angle Spinning 1H NMR Spectroscopy of Intact Liver and Kidney: Optimization of Sample Preparation Procedures and Biochemical Stability of Tissue during Spectral Acquisition

“…By spinning the sample at the magic angle ( ϭ 54.7°, the angle which the rotation axis makes with the magnetic field, B 0 ), the partially averaged chemical shift anisotropies and dipolar couplings, which are scaled by (3 cos 2 Ϫ 1)/2, go to zero (1). High-resolution 1 H MAS NMR spectroscopy has recently been employed to study the biochemistry of intact tissue samples such as renal cortex (2, 3), adipose tissue (4), intact red blood cells (5), prostate (6), and brain (7). 1 H MAS NMR spectroscopy can also be used to differentiate between malignant and benign tissue based predominantly on differences in the lipid signals (3,6,8).…”

“…High-resolution 1 H MAS NMR spectroscopy has recently been employed to study the biochemistry of intact tissue samples such as renal cortex (2, 3), adipose tissue (4), intact red blood cells (5), prostate (6), and brain (7). 1 H MAS NMR spectroscopy can also be used to differentiate between malignant and benign tissue based predominantly on differences in the lipid signals (3,6,8). In addition, extensive assignment of the 1 H NMR signals of liver (9) and renal cortex and medulla (10) has been carried out.…”

The mobility of chondroitin sulfate in articular and artificial cartilage characterized by 13C magic‐angle spinning NMR spectroscopy

“…However, HR MAS reduces the line-broadening effects of magnetic susceptibility differences in biological tissues, so that sharper NMR signals can be obtained than with conventional HR NMR techniques. 46 To analyze the motions of the chondroitin sulfate in native and artificial cartilage, we measured the longitudinal (T 1 ) and transverse (T 2 ) relaxation times of the molecules. All experimental data could be fitted using single exponential models.…”