High resolution liquid chromatography with a packed micro glass capillary column

Hirata, Y.; Jinno, Kiyokatsu

doi:10.1002/jhrc.1240060407

Cited by 69 publications

(26 citation statements)

References 10 publications

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“…The work of Novotny and co-workers [40][41][42], Yang [37] and Scott et al [44][45][46][47][48] are regarded as key publications in the field of microcolumn LC. The initial developments towards miniaturization in high-performance liquid chromatography were quickly followed by a number of laboratories worldwide [49][50][51].…”

Section: Introductionmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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This review discusses many different aspects of microcolumn liquid chromatography (LC) and reflects the areas of microcolumn LC research interest over the past decades. A brief theoretical discussion on a number of major issues, such as column characterisation, chromatographic dilution effects and extracolumn band broadening in microcolumn LC is given. Recent progress in column technology and the demands and developments of instrumentation and accessories for microcolumn LC are also reviewed. Besides that, the developments in a large number of established and also more recent detection techniques for microcolumn LC are also discussed. The potential of hyphenation of microcolumn LC with other techniques, more particularly of multidimensional chromatography and microcolumn LC coupled to mass spectrometry is reviewed. Finally, the perspective of microcolumn LC separation methods is stressed by a number of relevant applications.

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Section: Introductionmentioning

confidence: 99%

Microcolumn liquid chromatography: instrumentation, detection and applications

Vissers¹,

Claessens

Cramers

1997

Journal of Chromatography A

208

101

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“…Although varying temperatures are not limited to small ID columns, such columns are better candidates for rapid changes in the column temperature, either on switching between constant temperatures or on use of temperature gradients, due to their low thermal mass [35,80]. An example of high resolution of polymeric amines with MW of 1000 -9000 by a temperature program is shown in Figure 1, with both mass spectrometric and light scattering detection.…”

Section: Improved Temperature Controlmentioning

confidence: 98%

Capillary columns in liquid chromatography: between conventional columns and microchips

Saito

Jinno

Greibrokk³

2004

J of Separation Science

105

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Liquid chromatography on columns with small internal diameters has been reviewed as the intermediate technique between conventional liquid chromatography and microchip separations. The development of micro column separations in the early years has been described, starting with the papers of Horváth and co-workers and Ishii and co-workers, continuing into the first part of the eighties, then making a leap in time to recent innovations with small-bore columns. Based on internal diameters a classification of the different analytical HPLC columns has been suggested. The advantages of small-bore columns have been discussed, with particular emphasis on the advantage of coupling to concentration sensitive detectors when the sample amount is limited. Open tubular columns are treated as a part of the historic background. The recent developments include a brief look into the current status of monolithic columns, the use of packed nano columns and micro columns with electrospray mass spectrometry, and the potential of two-dimensional comprehensive liquid chromatography. Finally, the coupling of sample preparation to analytical columns and the future applications of the novel technological improvements to the microchip separation methods have been discussed.

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“…For example, fast separations are performed on columns typically 10-20 cm long (32), whereas high-resolution separations of complex mixtures will require column lengths of up to a few meters (26,27,(33)(34)(35). Some details of the preparation procedure have beenreported by many workers (14,26,33), but they vary considerably among laboratories. Some of them are even patented (36).…”

Section: Column Preparationmentioning

confidence: 98%

“…The balanced density method is not popular because of the toxicity of the solvent and difficulty in washing. The viscosity method, which often has been used for microbore columns (45), can also produce high efficiencies (26). Gluckman et al have reported that the viscosity method requires long packing times and produces a relatively loosely packed bed, rcsulting in voids at the column head during use (14).…”

Section: Column Preparationmentioning

confidence: 99%