High-Resolution Liquid Chromatography of Oligonucleotides on Nonporous Alkylated Styrene-Divinylbenzene Copolymers

Huber, Christian G.; Oefner, Peter J.; Bonn, Günther K.

doi:10.1006/abio.1993.1340

Cited by 156 publications

(98 citation statements)

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“…However, the order of the base did not agree with earlier published data, 34 for both the IB and the IBD columns. Moreover, an exchange of the elution order was also observed based on the difference in the pHs.…”

Section: Application To the Oligonucleotidecontrasting

confidence: 83%

“…The hydrophobicity contribution to the oligonucleotide retention is in the order C < G < A < T, which is in agreement with earlier published data from the usual RP-HPLC. 34 In other words, the addition of C or G does not increase oligonucleotide retention as dramatically as the addition of A, and particularly T, because of the hydrophobicity of C and of G are similar. 9 In present study, on the IB column, little change in the retention times with temperature was observed for the oligonucleotides examined at pH 4.5.…”

Section: Application To the Oligonucleotidementioning

confidence: 99%

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Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

et al. 2006

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Section: Application To the Oligonucleotidecontrasting

confidence: 83%

Section: Application To the Oligonucleotidementioning

confidence: 99%

Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

et al. 2006

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“…When injected in the DHPLC, heteroduplex PCR fragments are retained less than their homoduplex analogues and, therefore, emerge ahead of the intact homoduplexes. 21,22 Bi-directional sequencing of PCR products of all individuals that showed an aberrant SSCP and=or DHPLC pattern was performed with an automatic sequencer (ABI PRISM 310, Perkin Elmer, USA). The MC4R amino acid and nucleotide numbering are in agreement with Gantz et al 6 One-hundred lean children were used as controls.…”

Section: Methodsmentioning

confidence: 99%

Low frequency of melanocortin-4 receptor (MC4R) mutations in a Mediterranean population with early-onset obesity

et al. 2002

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BACKGROUND: Melanocortin-4 receptor (MC4R) mutations have been reported as the most common single genetic cause of obesity in some populations and it has been suggested that they may be responsible for more than 4% of early-onset obesity. OBJECTIVES: To verify the presence of mutations of the MC4R coding region in children from southern Italy with early-onset obesity. SUBJECTS AND METHODS: Two-hundred and eight unrelated obese children and adolescents were included in the study. The average age at obesity onset was 4.5 AE 2.6 y. MC4R coding region was screened using both single-strand conformation polymorphism (SSCP) analysis and denaturing high-performance liquid chromatography (DHPLC). Automatic sequencing of PCR products of all individuals that showed an aberrant SSCP and=or DHPLC pattern was performed. RESULTS: One novel missense mutation and one previously described polymorphism (Vall03Ile) were identified. The missense mutation C142T, resulting in the substitution of proline with serine at codon 48, within the first MC4R transmembrane domain, was detected at the heterozygous state in a 15-y-old obese girl (body mass index (BMI) ¼ 35 kg=m 2 ) who has been obese since she was 8 y old. The mutation co-segregated with the obesity phenotype for over three generations and was not found in the control population. CONCLUSIONS: Our data show MC4R obesity causing mutations in less than 0.5% of the patients (ie 1 out of 208 patients) and therefore indicate a low prevalence of MC4R variants in the obese population from southern Italy. The specific genetic background of the Mediterranean population could make it difficult for MC4R mutations to produce an essentially polygenic trait such as common obesity, at least during childhood.

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“…[17][18][19][20][21] In its early stage of application for the analysis of nucleic acids, dHPLC was shown to provide an effective means for separating oligonucleotides, 22 PCR fragments, 23 and for analyzing the products formed in competitive reverse-transcriptase PCR (RT-PCR) reactions to determine relative levels of gene expression. 24 Mutation scanning by dHPLC involves subjecting PCR products to ion-pair-reverse-phase liquid chromatography in a column containing alkylated nonporous particles.…”

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confidence: 99%

Evaluation of denaturing high‐performance liquid chromatography as a rapid detection method for identification of epidermal growth factor receptor mutations in nonsmall‐cell lung cancer

Cohen

Agulnik

Jarry

et al. 2006

Cancer

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Objectives: The polymorphic site rs4647905 of the FGFR1 gene was previously associated with a decrease in cephalic index (CI). Here, we evaluate the relationships between genotypes and cephalometric measurements and indices in one Mexican Native and two mestizo Mexican populations using two haplotype‐tag SNPs (rs4647905 and rs3213849) that represent >85% of the FGFR1 variability, plus three other SNPs (rs2293971, rs2304000, and rs930828) situated nearby. In addition, we genotyped five South American natives, two European, one African, and one Siberian populations to evaluate their intra and intercontinental population diversity. Methods: The five SNPs were tested and the craniofacial measurements and indices were collected using standardized procedures. Principal Component Analysis was used to verify individual/population comparisons. Associations were performed through the generalized linear model (GLM), coefficient of determination R2 and linear regression tests. Results: We found a tendency for a decrease in CI in individuals homozygous for allele rs4647905C, regardless of the population to which they belong, though the effect is more pronounced in mestizo. When the GLM analyses were performed using the absolute/linear cephalometric measurements, a statistically significant association was found between four SNPs and head length in the mestizo population. Conclusions: FGFR1 polymorphisms, especially rs4647905, can have an important role in the normal human skull variation, primarily due to their influence in head length, which would affect other cephalometric absolute/linear measures as well as indices like CI as a result of the pervasive nature of the morphological integration that characterizes the human skull. Am. J. Hum. Biol. 2013. © 2012 Wiley Periodicals, Inc.

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High-Resolution Liquid Chromatography of Oligonucleotides on Nonporous Alkylated Styrene-Divinylbenzene Copolymers

Cited by 156 publications

References 0 publications

Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

Separation of Nucleotides with an Aqueous Mobile Phase Using pH- and Temperature-Responsive Polymer Modified Packing Materials

Low frequency of melanocortin-4 receptor (MC4R) mutations in a Mediterranean population with early-onset obesity

Evaluation of denaturing high‐performance liquid chromatography as a rapid detection method for identification of epidermal growth factor receptor mutations in nonsmall‐cell lung cancer

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