High-Resolution Liquid Chromatography–Mass Spectrometry for Lipidomics

Harvey, Faith Christine; Collao, Vanessa; Bhattacharya, Sanjoy K.

doi:10.1007/978-1-0716-2966-6_4

Cited by 4 publications

(1 citation statement)

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“…As metabolomics can reflect the physiological or pathological state of organisms, it plays a vital role in studying the effects and underlying mechanisms of environmental chemical toxicity [ 15 ]. Liquid chromatography coupled to tandem mass spectrometry (LC-MS) is a powerful tool for identifying and classifying metabolomes for its high sensitivity and wide range of chemical detection coverage [ 16 ]. Therefore, it is necessary to employ metabolomics technology to investigate the effects of molybdate exposure on the body’s endogenous metabolism.…”

Section: Introductionmentioning

confidence: 99%

Exposure to Molybdate Results in Metabolic Disorder: An Integrated Study of the Urine Elementome and Serum Metabolome in Mice

Zhou,

Tang,

Zhang

et al. 2024

Toxics

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The increasing use of molybdate has raised concerns about its potential toxicity in humans. However, the potential toxicity of molybdate under the current level of human exposure remains largely unknown. Endogenous metabolic alterations that are caused in humans by environmental exposure to pollutants are associated with the occurrence and progression of many diseases. This study exposed eight-week-old male C57 mice to sodium molybdate at doses relevant to humans (0.01 and 1 mg/kg/day) for eight weeks. Inductively coupled plasma mass spectrometry (ICP-MS) and ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS) were utilized to assess changes in urine element levels and serum metabolites in mice, respectively. A total of 838 subjects from the NHANES 2017–2018 population database were also included in our study to verify the associations between molybdenum and cadmium found in mice. Analysis of the metabolome in mice revealed that four metabolites in blood serum exhibited significant changes, including 5-aminolevulinic acid, glycolic acid, l-acetylcarnitine, and 2,3-dihydroxypropyl octanoate. Analysis of the elementome revealed a significant increase in urine levels of cadmium after molybdate exposure in mice. Notably, molybdenum also showed a positive correlation with cadmium in humans from the NHANES database. Further analysis identified a positive correlation between cadmium and 2,3-dihydroxypropyl octanoate in mice. In conclusion, these findings suggest that molybdate exposure disrupted amino acid and lipid metabolism, which may be partially mediated by molybdate-altered cadmium levels. The integration of elementome and metabolome data provides sensitive information on molybdate-induced metabolic disorders and associated toxicities at levels relevant to human exposure.

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Section: Introductionmentioning

confidence: 99%