During the last glacial cycle, greenhouse gas concentrations fluctuated on decadal and longer timescales. Concentrations of methane, as measured in polar ice cores, show a close connection with Northern Hemisphere temperature variability, but the contribution of the various methane sources and sinks to changes in concentration is still a matter of debate. Here we assess changes in methane cycling over the past 160,000 years by measurements of the carbon isotopic composition δ 13 C of methane in Antarctic ice cores from Dronning Maud Land and Vostok. We find that variations in the δ 13 C of methane are not generally correlated with changes in atmospheric methane concentration, but instead more closely correlated to atmospheric CO 2 concentrations. We interpret this to reflect a climatic and CO 2 -related control on the isotopic signature of methane source material, such as ecosystem shifts in the seasonally inundated tropical wetlands that produce methane. In contrast, relatively stable δ
13C values occurred during intervals of large changes in the atmospheric loading of methane. We suggest that most methane sources-most notably tropical wetlands-must have responded simultaneously to climate changes across these periods.C limate variations over the past glacial cycle are characterized by global temperature changes 1,2 , sea-level fluctuations 3,4 and substantial changes in atmospheric greenhouse gas concentrations 5 . Abrupt climate shifts, for example DansgaardOeschger events, characterize much of the glacial records in the Northern Hemisphere and are mirrored in the icecore CH 4 record 6,7 . The nature of the CH 4 -climate coupling on glacial-interglacial and millennial timescales is still a matter of debate 7,8 . Studies of the interpolar CH 4 concentration difference using ice cores from both polar regions are interpreted as a constraint of the latitudinal distribution of CH 4 emission sources 9,10 . Furthermore, most CH 4 sources/sinks have characteristic isotope signatures. Accordingly, atmospheric CH 4 isotope records provide refined boundary conditions to constrain changes in individual sources or sinks over time [11][12][13] . Here we present CH 4 isotope data from ice cores covering the past 160,000 years (160 kyr; Fig. 1), thereby extending the atmospheric δ 13 CH 4 record to a full glacial-interglacial cycle. Generally, our record confirms increased δ 13 CH 4 values under full glacial conditions 11 , decreasing δ 13 CH 4 during terminations (except during the unique Younger Dryas cold reversal) and the continuation of this declining trend over the following interglacial 13 , irrespective of the CH 4 evolution. Although an unambiguous alignment of termination I and II is not possible owing to the Younger Dryas event, δ 13 CH 4 values of the two deglaciations seem to be offset by ∼2 (Fig. 2) Fig. 3). In stark contrast to the MIS 5-4 transition, rapid CH 4 changes during Dansgaard-Oeschger events are not imprinted in the δ 13 CH 4 record. Comparing the full δ 13 CH 4 record with other global climate reco...