High‐resolution in vivo imaging of microglia using a versatile nongenetically encoded marker

Schwendele, Bernd; Brawek, Bianca; Hermes, Marina; Garaschuk, Olga

doi:10.1002/eji.201242436

Cited by 33 publications

(37 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A concentration of 1-100 M (bath application) was effective in inducing intracellular Ca 2+ transients in cultured microglia [52]. P2X 7 is also highly expressed in microglial cells in vivo, as 100% of tested microglia responded to a local pressure application of Bz-ATP (5 mM [43] or 500 M [47]) with a Ca 2+ transient.…”

Section: Agonist-induced Ca 2+ Signalingmentioning

confidence: 95%

See 1 more Smart Citation

Microglial calcium signaling in the adult, aged and diseased brain

Brawek

Garaschuk

2013

Cell Calcium

Self Cite

View full text Add to dashboard Cite

Section: Agonist-induced Ca 2+ Signalingmentioning

confidence: 95%

“…Oregon Green BAPTA-1) by means of single cell electroporation [43,47]. Also here the electroporation procedure (although using very mild current injection protocol) [43] might have modified the functional properties of microglia under study.…”

Section: Microglial Ca 2+ Signalingmentioning

confidence: 99%

Microglial calcium signaling in the adult, aged and diseased brain

Brawek

Garaschuk

2013

Cell Calcium

Self Cite

View full text Add to dashboard Cite

“…Within stained cortical areas, 100 % of GFP-positive cells were also positive for Tomato lectin [ 54 ].…”

Section: Visualization Of Microglia By Means Of Plant Lectinsmentioning

confidence: 97%

“…Thiofl avin-S is a blue dye which binds to amyloid β and thus specifi cally labels senile plaques. Panel ( a ) is reproduced from [ 25 ] with permission from Elsevier; panels ( b )-( d ) are reproduced from [ 54 ].…”

Section: Visualization Of Microglia By Means Of Plant Lectinsmentioning

confidence: 99%

See 1 more Smart Citation

Imaging Morphology and Function of Cortical Microglia

Brawek

Garaschuk

2013

Neuromethods

Self Cite

View full text Add to dashboard Cite

Microglial cells are the innate immune cells of the central nervous system. In the healthy adult brain "resting" ramifi ed microglia continuously palpate their environment to monitor the integrity of and to react to any disturbance of brain homeostasis. During injury, infl ammation, and in the course of neurodegenerative diseases microglia become activated, proliferate, and release a plethora of cytokines as well as reactive oxygen species. In addition to their well known role in disease, it has become increasingly clear that "resting" microglia also contribute to normal brain physiology, both during postnatal development and in the mature adult brain.Functional in vivo imaging of microglia fi rst of all captures the morphological changes accompanying microglial transition between "resting" and activated states. In addition, intracellular Ca 2+ homeostasis of microglia is believed to be altered between the two states [1][2][3]. So far, however, microglial Ca 2+ signaling was predominantly studied in reduced preparations like brain slices or cell cultures, in which microglia are found in a rather activated state. In this chapter we describe a technique for studying microglial Ca 2+ signaling in vivo. Furthermore, we discuss a new approach for visualization of morphological dynamics of microglial cells in vivo at high resolution. This approach utilizes a lectin-based staining technique and is applicable to any deliberate mouse strain at any developmental stage.

show abstract

The future of immunoimaging — Deeper, bigger, more precise, and definitively more colorful

et al. 2013

View full text Add to dashboard Cite

Immune cells are thoroughbreds, moving farther and faster and surveying more diverse tissue space than their non-hematopoietic brethren. Intravital 2-photon microscopy has provided insights into the movements and interactions of many immune cell types in diverse tissues, but much more information is needed to link such analyses of dynamic cell behavior to function. Here we describe additional methods whose application promises to extend our vision, allowing more complete, multiscale dissection of how immune cell positioning and movement are linked to system state, host defense, and disease.

show abstract

High‐resolution in vivo imaging of microglia using a versatile nongenetically encoded marker

Abstract: Keywords: Alzheimer's disease r In vivo imaging r Microglia r Tomato lectin

Cited by 33 publications

References 18 publications

Microglial calcium signaling in the adult, aged and diseased brain

Microglial calcium signaling in the adult, aged and diseased brain

Imaging Morphology and Function of Cortical Microglia

The future of immunoimaging — Deeper, bigger, more precise, and definitively more colorful

Contact Info

Product

Resources

About