High resolution imaging of bio-molecular binding studies using a widefield surface plasmon microscope

Imaging of the cell surface interface using objective coupled widefield surface plasmon microscopy

“…A recent study (Jamil et al, 2008) has shown that the widefield surface plasmon microscope can be used to differentially resolve the interactions between antibodies and nanometrically thick layers of the extracellular matrix proteins laminin and fibronectin. These binding events of course cause localised changes in the mean refractive index which can be measured either as changes in contrast, or by monitoring the change in incidence angle required to promote optimal coupling of p-polarised light into the surface plasmons.…”

Dynamics of plasmon in graphene oxide

“…Ozaki et al, created a hologram technique which is readable under visible light using metal nanostructures with excitation of surface plasmons. 1 Applications of plasmons can be realized in waveguides, 2 energy harvesters, 3 bio-molecule binders 4 and drug delivery systems. 5 Recent progress on plasmonic research, going towards tuning the energy range of plasmon excitations which cover wide range of applications.…”

Localized surface plasmon assisted contrast microscopy for ultrathin transparent specimens

“…1 Besides the aforementioned conventional high contrast microscopy techniques, propagating surface plasmon (SP) or localized SP assisted contrast enhancement techniques were also explored during past few decades. [4][5][6][7][8][9][10][11][12][13][14][15][16][17][18] SP is the collective oscillation of electrons propagating on metal surface (propagating SP) or confined to metallic-nanostructures (localized SP). Since the excitation conditions for both propagating and localized SP resonances are very sensitive to the local refractive index variation, 2,3 they are widely used in label-free bio-sensing 4-6 and bio-imaging.…”

“…Since the excitation conditions for both propagating and localized SP resonances are very sensitive to the local refractive index variation, 2,3 they are widely used in label-free bio-sensing 4-6 and bio-imaging. [7][8][9][10][11][12][13][14][15][16][17][18] In the field of microscopy, a propagating SP assisted microscopy technique was first demonstrated in late 1980s and greatly improved the image contrast for thin-film samples with non-uniform refractive index. 7,8 However, its spatial resolution is limited by the propagation length of SPs and is on the order of microns.…”

“…7,8 To improve the spatial resolution, microscope objectives were then used to excite propagating SP and several scanning or wide field approaches have been demonstrated. [9][10][11][12][13][14][15] The scanning approaches 9-11 achieve relatively higher spatial resolution ($200-300 nm), but sacrifice the image acquisition time compared with the wide field techniques. [12][13][14][15] Compared with the propagating SP assisted microscopy techniques, localized SP assisted imaging techniques intrinsically possess high spatial resolution, because the localized SP field is confined within tens to hundreds of nanometers region around the deep sub-diffraction-limited nanostructures and only the local refractive indices influence the LSPR characteristics.…”